Significance of measuring oxidative stress in lifestyle-related diseases from the viewpoint of correlation between d-ROMs and BAP in Japanese subjects

Abstract

In recent years, oxidative stress has been postulated to be an important factor in the pathogenesis and development of lifestyle-related diseases. In this study, we investigated the association between the derivatives of reactive oxygen metabolites (d-ROMs), as an index of products of reactive oxygen species (ROS), and biological antioxidant potential (BAP), as an index of antioxidant potential. We also investigated the associations between d-ROMs or BAP and the risk factors for lifestyle-related diseases or metabolic syndrome-associated factors to evaluate their usefulness in preventive medicine. There were 442 subjects who underwent health checkup examination in our facilities. In addition to standard medical checkup items, we analyzed d-ROMs, BAP, brachial-ankle pulse wave velocity, high-sensitivity C-reactive protein level and visceral fat area (VFA) visualized on a computed tomography scan. The mean d-ROM value in females was significantly higher than that in males. There was a positive correlation between the d-ROM and VFA levels. On correlation analysis, there was a negative correlation between the d-ROM and creatinine levels. As factors that influence d-ROMs, the level of VFA was selected, suggesting the significance of oxidative stress measurement with d-ROMs. In addition, there was a positive correlation between d-ROMs and BAP values. Further research is required to resolve whether increased production of ROS or the antioxidant potential that can compensate for such an increase of ROS is more important in vivo. INTRODUCTIONOxidative stress may be involved in various disorders and pathogeneses, especially in lifestyle-related diseases, and it causes the progression and acceleration of atherosclerosis. 1-3 Biomarkers of oxidative stress, such as serum malondialdehyde level measured as thiobarbituric acid-reactive substances, oxidized low-density lipoprotein, oxidation injury of DNA, 8-hydroxydeoxyguanosine and urinary excretion of 8-iso-prostaglandin-F2a, are generally measured in laboratories. [4][5][6][7] On the other hand, as indices of antioxidant potential, intracellular levels of superoxide dismutase and glutathione peroxidase have mainly been measured. [8][9] However, these assay methods are complex and are not suitable for analysis of a large number of subjects. Recently, simpler methods for detecting reactive oxygen species (ROS) using derivatives of reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) were developed, and reports on studies using these methods have been increasing. [10][11][12][13][14][15][16][17][18][19][20] Thus, in the present study, we measured d-ROMS and BAP as indices for the production of ROS and antioxidant potential, respectively.

049 Physiological and molecular mechanisms associated with performance, immunometabolic status, and liver function in transition dairy cows fed rumen-protected methionine or choline

“…In fact, ROM has been widely-adopted as a biomarker for oxidative stress in both non-ruminants (Fukui et al, 2011, Jansen et al, 2013 and ruminants (Bernabucci et al, 2002, 2005, Trevisi et al, 2009, Celi et al, 2010. Although ROM concentrations in the present study were not affected by MET or CHOL supplementation, the substantially higher ROM starting at 4 d postpartum was indicative of an increased risk for the onset of oxidative stress.…”

“…The increase in pro-oxidant levels is usually associated with increased risk of oxidative stress and therefore may be used as biomarkers. Because ROS concentration is an indicator of free radical production (Miller et al, 1993, Sugino, 2006, and when in excess can compromise cell function through damaging cellular lipid, protein, and DNA, it has been widely adopted as a biomarker for oxidative stress in non-ruminants (Fukui et al, 2011, Leufkens et al, 2012, Jansen et al, 2013 and ruminants (Bernabucci et al, 2002, 2005, Celi et al, 2010). In fact, the level of ROS is now considered as the gold standard for assessing total oxidative status (Celi, 2010).…”

Association between Urinary 8-Hydroxydeoxyguanosine, an Indicator of Oxidative Stress, and the Cardio-Ankle Vascular Index in Hypertensive Patients

“…It has been suggested that enhanced oxidative stress may be closely related to the pathogenesis and development of arteriosclerosis [1][2][3][4] ; therefore, biomarkers of oxidative stress, including thiobarbituric acid-reactive substances 5) , oxidized low-density lipoprotein 6) , and reactive oxygen species (ROS), measured as derivatives of reactive oxygen metabolites (d-ROMs) 7) , are generally measured in patients with atherosclerosisrelated diseases; however, these assay methods require blood sampling.…”

The Apolipoprotein B/A1 Ratio is Associated with Reactive Oxygen Metabolites and Endothelial Dysfunction in Statin-Treated Patients with Coronary Artery Disease

“…The d-ROMs level is expressed in an arbitrary unit called the Carratelli unit (U.CARR) 9) . The d-ROMs test is not a fully established standard method of measuring oxidative stress; however, it has been recognized as an emerging plasma biomarker for the prediction of cardiovascular disease [10][11][12][13] . We measured the percent flow-mediated dilatation (%FMD) as a marker of endothelial dysfunction.…”