Signal amplified two-dimensional photonic crystal biosensor immobilized with glyco-nanoparticles

Near-infrared surface plasmon resonance imaging (SPRI) microscopy is used to detect and characterize the adsorption of single polymeric and protein nanoparticles (PPNPs) onto chemically modified gold thin films in real time. The single-nanoparticle SPRI responses, Δ%RNP, from several hundred adsorbed nanoparticles are collected in a single SPRI adsorption measurement. Analysis of Δ%RNP frequency distribution histograms is used to provide information on the size, material content, and interparticle interactions of the PPNPs. Examples include the measurement of log-normal Δ%RNP distributions for mixtures of polystyrene nanoparticles, the quantitation of bioaffinity uptake into and aggregation of porous NIPAm-based (N-isopropylacrylamide) hydrogel nanoparticles specifically engineered to bind peptides and proteins, and the characterization of the negative single-nanoparticle SPRI response and log-normal Δ%RNP distributions obtained for three different types of genetically encoded gas-filled protein nanostructures derived from bacteria.

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“… 56 It has been previously demonstrated that mannose-modified HNPs have K d values in the micromolar to nanomolar range. 57 …”

Section: Resultsmentioning

confidence: 99%

Characterizing Single Polymeric and Protein Nanoparticles with Surface Plasmon Resonance Imaging Measurements

Maley

Shapiro

et al. 2017

ACS Nano

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“…The TMSMA and NAS moieties act as surface-reactive and amine-reactive groups, respectively, and poly(TMSMA-r-NAS) has frequently been used for binding a sensor matrix and protein ligands via the amine coupling reaction. [16][17][18] Anti-human hemoglobin monoclonal antibodies and purified human hemoglobin were purchased from Nippon Bio-Test Laboratories Inc. (Tokyo, Japan) and MP Biomedicals, LLC (Santa Ana, CA), respectively. Albumin from bovine serum (Sigma Co., St. Louis, MO) was used as a negative-control protein.…”

Section: Reagents and Materialsmentioning

confidence: 99%

Label-free Detection of Antigen Protein Using a Metal Mesh Device Surface-modified by an Antibody

et al. 2015

Self Cite

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A biosensor for protein detection was developed using antibody-immobilized metal mesh devices. Antihemoglobin antibodies were covalently immobilized on a metal mesh device. Extraordinary transmission with a dipped structure was observed for a metal mesh device immobilized with antihemoglobin antibodies as well as for the original metal mesh device. Hemoglobin in the mixture solution containing albumin at a hundred-fold concentration was detectable using antihemoglobin-immobilized MMDs. The detectability using the antihemoglobin-immobilized metal mesh device was similar to that of a commercially-available kit for the qualitative determination of hemoglobin.

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“…49 By monitoring the change in the intensity of the reflected light from the PhC, detection limits of 16.7 ng/mL and 6.0 ng/mL were reported for ConA binding to ManHP and ManNP, respectively. The lower detection limit in the case of ConA binding to ManNP was attributed to the three-fold increase in the binding capacity of nanogel particles in comparison with ManHP immobilized directly on the slab-PhC.…”

Section: Resultsmentioning

confidence: 99%

Two-dimensional photonic crystals for sensitive microscale chemical and biochemical sensing

2015

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Photonic crystals – optical devices able to respond to changes in the refractive index of a small volume of space – are an emerging class of label-free chemical-and bio-sensors. This review focuses on one class of photonic crystal, in which light is confined to a patterned planar material layer of sub-wavelength thickness. These devices are small (on the order of tens to 100s of microns square), suitable for incorporation into lab-on-a-chip systems, and in theory can provide exceptional sensitivity. We introduce the defining characteristics and basic operation of two-dimensional photonic crystal sensors, describe variations of their basic design geometry, and summarize reported detection results from chemical and biological sensing experiments.

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Signal amplified two-dimensional photonic crystal biosensor immobilized with glyco-nanoparticles

Abstract: A two-dimensional, glycopolymer-immobilized, photonic crystal (PhC) biosensor was developed for the detection of proteins.

Cited by 27 publications

References 47 publications

Characterizing Single Polymeric and Protein Nanoparticles with Surface Plasmon Resonance Imaging Measurements

Characterizing Single Polymeric and Protein Nanoparticles with Surface Plasmon Resonance Imaging Measurements

Label-free Detection of Antigen Protein Using a Metal Mesh Device Surface-modified by an Antibody

Two-dimensional photonic crystals for sensitive microscale chemical and biochemical sensing

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