Label-free Detection of Antigen Protein Using a Metal Mesh Device Surface-modified by an Antibody

Seto, Hirokazu; Kamba, Seiji; Kondo, Takashi; Ogawa, Yuichi; Hoshino, Yu; Miura, Yoshiko

doi:10.2116/analsci.31.173

Cited by 12 publications

(10 citation statements)

References 18 publications

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“…The dip in the spectra were specific phenomena in MMD, which have been reported by our group [13][14][15] and other groups. 8,9 The dip was the result of fano-like interference effect between the terahertz light transmitted directly and obliquely through MMD.…”

Section: Measurements and Calculations Of Transmittance Properties Ofsupporting

confidence: 79%

“…In the experiments, protein adsorption caused the dip frequency shift. [13][14][15] The magnetic permeability of the organic substances is usually less than 1. The dip frequency shift from the inductance change can be ignored due to the small magnetic permeability.…”

Section: Equivalent Circuit Of MMDmentioning

confidence: 99%

“…In 2011, we reported the sensing principles of MMD in the sub-millimeter range, 12 and the possibility of protein sensing adsorbed onto the MMD by the transmittance property in the terahertz (infrared) region. [13][14][15] The MMD was manufactured with the electroforming method, which enables the fabrication of the devices of various structures, sizes, and transmittance properties of arbitrary frequency (wavelength). Therefore, it is possible to utilize the specific MMD corresponding to the target substances.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Measurement of Protein Using Metal Mesh Device

et al. 2017

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Biosensing of protein adsorption with metal mesh device (MMD) was investigated by computational calculations and experiments. Electromagnetic field computation was carried out with a single unit cell of MMD. Equivalent circuit model of MMD on the single unit cell was assumed, and the biosensing with MMD was analyzed in detail by computational calculation and experimental measurements. The dip frequency of MMD was shifted by adsorption of protein on MMD. The shift of dip frequency of MMD was proportional to the amount of protein adsorption. The sensitivity of MMD biosensing was dependent on the microstructure of MMD, and proportional to the square of the dip frequency. The refinement of MMD structure can improve the sensitivity of protein detection.

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Section: Measurements and Calculations Of Transmittance Properties Ofsupporting

confidence: 79%

Section: Equivalent Circuit Of MMDmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantitative Measurement of Protein Using Metal Mesh Device

et al. 2017

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“…[1][2][3][4][5] Recently, we developed a label-free detection method for biomolecules using our fabricated micro-and nanofluidic device. 6 This detection method is based on optical diffraction that occurs when the laser beam passes through nanostructures inside the micro-and nanofluidic device.…”

Section: Introductionmentioning

confidence: 99%

Using Laser Interference Lithography in the Fabrication of a Simplified Micro- and Nanofluidic Device for Label-free Detection

et al. 2017

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Recently, we developed a label-free detection method based on optical diffraction, and implemented it in on our fabricated micro-and nanofluidic device. This detection method is simple and useful for detecting biomolecules, but the device fabrication consists of complicated processes. In this paper, we propose a simple method for fabricating the micro-and nanofluidic device; the fabrication combines laser interference lithography with conventional photolithography. The performance of a device fabricated by the proposed method is comparable to the performance of the device in our previous study.

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“…When the complex refractive index is modified by adherence of a particulate substance to the metal surface, localized electric fields are influenced, and transmission patterns are altered in a frequency-sensitive manner ( Figure 2). Based on this phenomenon, MM membranes can be employed as label-free optical sensors [2][3][4][5]. Accordingly, MM sensors have attracted considerable attention because of their prospective use as simple, inexpensive biosensors.…”

Section: Introduction (Heading 1)mentioning

confidence: 99%

Use of metal mesh sensors with periodic microstructures to sense and separate aerosol particles in Kenya

et al. 2015

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This study introduces a new optical metal mesh(MM) sensor for detection of aerosol particles. The base material of the sensor corresponds to a thin metal membrane with unique optical properties and a micromesh structure. A small air pump pulls air through the MM membrane, permitting capture of aerosol particles on the micromesh surface and subsequent detection of the particles via infrared (IR) techniques. Here, MM membranes with different square apertures (side length: 4.0, 1.8, or 1.1 μm) were combined to form two-membrane stacked MM sensors. The sensors were used to collect particulate matter 2.5 (PM2.5; average diameter: ≤ 2.5 μm) as an indication of environmental pollution in the suburbs of Nairobi, Kenya. The elemental composition of each particle within captured PM2.5 was determined via wavelength-dispersive X-ray spectrometry. The results suggest that stacked MM sensors can successfully provide information about aerosol particle pollutants in the local environment.

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Label-free Detection of Antigen Protein Using a Metal Mesh Device Surface-modified by an Antibody

Cited by 12 publications

References 18 publications

Quantitative Measurement of Protein Using Metal Mesh Device

Quantitative Measurement of Protein Using Metal Mesh Device

Using Laser Interference Lithography in the Fabrication of a Simplified Micro- and Nanofluidic Device for Label-free Detection

Use of metal mesh sensors with periodic microstructures to sense and separate aerosol particles in Kenya

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