This study was conducted to evaluate supplementation of Mangosteen (Garcinia mangostana) pericarp filtrate in a tris-egg yolk-based diluent on buck sperm membrane integrity after cooling. Semen was collected from 5 bucks by artificial vagina method. Fresh semen evaluated for colour, pH, volume, concentration, mass motility, motility, viability, sperm abnormality and sperm membrane integrity. Semen was diluted with tris-egg yolk-based extender supplemented with different levels Mangosteen (Garcinia mangostana) pericarp filtrate (0, 10, 20 and 30 %) v/v with the ratio of 1 semen : 9 diluter. Semen used mass motility of 2+ and motility of 70%. Immediately after dilution semen was stored for 4ď‚°C and sperm membrane integrity percentage were observed at 0, 24, 48 h and 72 h. The obtained data were analyze with Analysis of Variant (ANOVA) and Least Significant Difference was determined. The experiment was designed using completely random design ( 4 treatments and 10 replications). The results showed that the level of mangosteen pericarp filtrate had very significant effect (P<0,01) on sperm membrane integrity percentage in 0, 24, 48 and 72 h of cooling. It concluded that the best mangsteen pericarp filtrate level for resulting optimal sperm membrane integrity was 10%.
Key words: AI, antioxidant, dilution, mangosteen, semen quality
INTRODUCTIONArtificial Insemination (AI) is one of the reproductive technologies that is very efficient and effective in supporting programs of genetic improvement of livestock. To support the efficiency of the AI, sufficient quantity and good quality of semen need to be available when required. In reality, not all male animals have a good genetic quality, so to obtain prospective candidate bucks as the source of germplasm must be strictly selected for excellence and females must come from parents of good quality. Thus, the superiority of males are expected to be passed on to offspring produced in significant amounts is limited, unless it is possible to serve the AI in females with large numbers, using semen be diluted with a good quality extender for the life of sperm (Isnaini, 2006). Attempts to dilute the buck semen and sustain the quality of sperm after cooling to crossbreed etawah bucks in rural areas is difficult as laboratory facilities for freezing buck semen are not available. Under conditions of simple equipment ownership in rural areas, AI breeders can work independently so that good-quality males that there can be more efficiently utilized.