Penelitian bertujuan untuk mengetahui pengaruh konsentrasi temulawak atau temulawak dan jahe terhadap sifat fisikokimia dan aktivitas antioksidan madu temulawak. Madu temulawak dibuat dari madu dan rempah yaitu ekstrak temulawak tanpa dan dengan penambahan jahe. Variasi konsentrasi ekstrak rempah yang digunakan adalah 1,5 sampai 7,5%. Total fenol, pH, total asam tertitrasi serta antioksidan dianalisis pada produk ini. Hasil penelitian menunjukkan bahwa penambahan jahe tidak memberikan pengaruh terhadap sifat fisikokimia tetapi cenderung menurunkan aktivitas antioksidan. Peningkatan konsentrasi temulawak maupun jahe dan temulawak meningkatkan kadar total fenolik dan pH serta menurunkan total asam tertitrasi dan total padatan terlarut. Peningkatan konsentrasi temulawak maupun jahe dan temulawak dapat meningkatkan antioksidan namun pada konsentrasi tertentu dengan hasil terbesar yaitu 81,43 % dan dengan IC50 sebesar 8247 ppm. Kesimpulannya, ekstrak jahe dapat meningkatkan aktivitas antioksidan dan sifat fisikokimia madu temulawak walaupun menunjukkan adanya peningkatan spesifik seiring dengan peningkatan konsentrasi penambahannya.Antioxidant Activity and Physicochemical Properties of Curcuma zanthorrhiza Roxb added with Extract of Ginger (Zingiber officinale Rosc)AbstractThe purpose of this study is to determine the effect of the concentration of temulawak or temulawak and ginger on the physicochemical properties and antioxidant activity of temulawak honey. Temulawak honey was made from honey, temulawak and ginger extract. The extract of 1.5 to 7.5% was used to produce temulawak honey beverage. Phenol content, pH, titratable acidity, and antioxidant activity was analyzed. The results showed that the addition of ginger had no effect on physicochemical properties but tended to increase antioxidant activity. The increase in concentration of temulawak and ginger might increase the total phenolic and pH and decreased the total titrated acid and total dissolved solids, however might increase antioxidant activity of temulawak honey at 81.43% with an IC50 of 8247 ppm. As conclusion, the ginger extract might increase antioxidant activity and physicochemical properties of beverage made from Curcuma zanthorrhiza Rosc.
Annato (Bixa orrelana L.) has been renowned as a tropical plant rich in carotenoid pigments such as nonpolar bixin and polar norbixin. This study was aimed to obtain natural colorant and antimicrobials from annatto extracts. The extraction was carried out by maceration for 10 mins using distilled water as the extraction solvent at various pH and extraction temperatures. The variations of solvent pH used in this research were 4, 7, and 9, while that of extraction temperatures were 70, 80, and 90oC. The potential of annatto extract as an antimicrobial agent was tested by analyzing the extract's ability to inhibit pathogens and its phytochemical compounds. Escherichia coli and Staphylococcus aureus were used as the pathogenic bacteria by using the agar diffusion method. The color of annatto extracts was measured using Munsel Chard to determine the level of hue (color), value (brightness), and chroma (intensity), as well as maximum absorbance. The results showed that all extracts have the potential to inhibit E. coli and S aureus (weak-moderate). The observed annatto extracts had different color intensities as indicated by the hue, value, and chroma and a maximum absorbance at a wavelength of 400 nm.
Bixa orellana known as annatto is often used as a natural colorant in some food industries. This pigment is potential as an antioxidant due to high levels of total phenol and carotenoid in seeds. This study aims to evaluate the effect of polarity of solvents in annatto extraction on colour and antioxidant content in the extracts as well as the application of extracts of annatto on chicken sausage products. The solvent used for extraction annatto was hexane (nonpolar), chloroform (semipolar) and ethanol (polar), and concentration of extracts used in the making of chicken sausage were 1, 2 and 3% respectively. Colour observation of extract was done visually and by using a tintometer to measuring the color components while the colour observation of chicken sausage was done using Munsell chard as well as sensory evaluation by the panelist. The antioxidant content in the extracts as well as in the chicken sausages was evaluated by measuring the total phenol and carotenoid. The result of this research shows that hexane extract had a yellow colour, which composed of red and yellow colour with intensity 9 and 37. The chloroform extract had red dense colour, which composed of red, yellow and blue with intensity 12, 4 and 6.9, respectively, while the ethanol extract had orange colour, which composed of red and yellow colour with intensity of 12, and 50. The highest content of total phenol (115 mg/g) was found in ethanol extract while the highest content of carotenoid (11.77mg/g) was produced in hexane extract. Addition of 2 % hexane extract gives the best colour on the chicken sausage, and based on the measuring with the Munsell chard was produced orange colour with hue 2.5 Y; value 7; and chrome 6, with the level of joy is like (3.8). The highest total phenol content (29.95 mg/g) was found in the chicken sausage which was added of 1% extract of hexane whereas the highest carotenoid content (16.17 ug/g) found in the chicken sausage which was added of 3 % extract of hexane.
The Siam orange (Citrus nobilis L.) has low economic value because of its sour taste and unattractive appearance. However, processing the fruit into nectar can increase its value. Nectar production requires the addition of sugar and the appropriate stabilizer for consumer acceptance. This research aimed to study the ideal combination of sucrose and stabilizer in Siam orange nectar production. A factorial completely randomized design was employed with two factors, the concentration of sucrose (10%, 15%, and 20%) and the type of stabilizer (CMC, carrageenan, pectin). As more sucrose was added to the juice, the moisture content decreased and the volume of soluble solids increased. The type of stabilizer only affected nectar pH and viscosity. CMC produced the highest nectar pH, followed by carrageenan and pectin. However, based on the results of the analysis of all parameters using the effectiveness index, carrageenan was the best stabilizer. Orange nectar with 20% sucrose and 0.5% carrageenan contained 24.2% total soluble solids, 76.6% moisture content, 7.9% reducing sugar, 23.5% vitamin C, and 3.38% crude fibre, with a viscosity of 109 cP and pH of 3.7. The product’s sensory characteristics were an orange colour, a sweet, citrus aroma, and a slightly thick texture. Additional research on the storage stability of this nectar formulation is necessary.
Coconut sap drink yogurt is a fermented product based on coconut sap and skim milk that made by fermentation using lactic acid bacteria of Lactobacillus bulgaricus and Streptococcus thermophilus. This study aimed to determine the effect of concentration of coconut sap and skim milk on physicochemical and sensory characteristic of coconut sap drink yogurt. The medium fermentation consists of 5% and 10% of skim milk and 0, 10, 30 and 50% of coconut sap. Ten percent of lactic acid bacteria was inoculated into media and it incubated for 6 hours at 37°C. The pH, total acid, reducing sugar, total sugar, viscosity, and sensory characteristic of the product were determined. The results showed that concentration of coconut sap and skim milk affected to pH, total acid, reducing sugar, total sugar, viscosity and sensory characteristic of coconut sap drink yogurt. The combination of 10% skim milk and 50% of coconut sap produce better characteristic of coconut sap drink yogurt than other combination. The product showed similar characteristic with commercial yogurt.
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