Shrimp (Pandalopsis dispar) waste hydrolysate as a source of novel β–secretase inhibitors

Abstract

In this study, purified peptides from shrimp waste hydrolysates (SWHs) were examined for their inhibitory effects against β-secretase. During consecutive purification using a Sephadex G-25 column chromatography and high performance liquid chromatography on a C18 column, a potent β-secretase inhibitory peptide Asp-Val-Leu-PheHis (629 Da) was isolated and identified from SWH24 by Q-TOF MS/MS and the IC 50 value was determined to be 92.70 μM. The β-secretase inhibition patterns of the purified peptides were found to be competitive. Among synthesized β-secretase inhibitory peptides, Leu-Phe-His had higher β-secretase inhibitory activity than the others. The result of this study suggests that the β-secretase inhibitory peptide derived from SWH24 could be potential candidates to develop nutraceuticals and pharmaceuticals.

Peptides: Production, bioactivity, functionality, and applications

“…Therefore, inhibition of b-secretase can be a potentially effective way to control and prevent AD. A bioactive peptide, with the amino acid sequence of LFH, from shrimp waste with inhibitory activity against b-secretase was recently reported (Li-Chan et al, 2016). Zou et al (2015) claimed that bioactive peptides obtained from porcine cerebral hydrolysate have potential ability to protect against memory impairment caused by Pb 2C presumably by reducing the Pb 2C concentration of the blood and brain (Zou et al, 2015).…”

“…There are two types of antithrombotic agents: anticoagulants and antiplatelets. The former prevent the formation and growth of clots while the latter inhibit platelet clumping (Li-Chan et al, 2016).…”