Short-term stability studies of ampicillin and cephalexin in aqueous solution and human plasma: Application of least squares method in Arrhenius equation

Nascimento, Ticiano Gomes do; Oliveira, Eliza; Júnior, Irinaldo Diniz Basílio; Araújo-Júnior, João Xavier de; Macêdo, Rui Oliveira

doi:10.1016/j.jpba.2012.04.010

Cited by 22 publications

(19 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By this we were able to reassure the presence of intact ampicillin during the measurements and to clearly distinguish between OmpF current modulation by native ampicillin and its degradation product (penicilloic acid) (12). Our obtained 1 H NMR spectra compare well with the previously published relevant studies (9,(12)(13)(14)(15)(16) and support our conclusions regarding modulation of the OmpF channel currents by ampicillin. Furthermore, at pH 8 ampicillin exhibits a charge state that allows us to directly apply the reversal potential permeation assay (21,26) to quantify ampicillin flux through the OmpF pore.…”

supporting

confidence: 86%

See 1 more Smart Citation

Ampicillin permeation across OmpF, the major outer-membrane channel in Escherichia coli

Ghai

Bajaj

Bafna

et al. 2018

Journal of Biological Chemistry

View full text Add to dashboard Cite

The outer cell wall of the Gram-negative bacteria is a crucial barrier for antibiotics to reach their target. Here, we show that the chemical stability of the widely used antibiotic ampicillin is a major factor in the permeation across OmpF to reach the target in the periplasm. Using planar lipid bilayers we investigated the interactions and permeation of OmpF with ampicillin, its basic pH-induced primary degradation product (penicilloic acid), and the chemically more stable benzylpenicillin. We found that the solute-induced ion current fluctuation is 10 times higher with penicilloic acid than with ampicillin. Furthermore, we also found that ampicillin can easily permeate through OmpF, at an ampicillin gradient of 10 μm and a conductance of ≅ 3.8 fS, with a flux rate of roughly 237 molecules/s of ampicillin at = 10 mV. The structurally related benzylpenicillin yields a lower conductance of ≅ 2 fS, corresponding to a flux rate of ≈120 molecules/s. In contrast, the similar sized penicilloic acid was nearly unable to permeate through OmpF. MD calculations show that, besides their charge difference, the main differences between ampicillin and penicilloic acid are the shape of the molecules, and the strength and direction of the dipole vector. Our results show that OmpF can impose selective permeation on similar sized molecules based on their structure and their dipolar properties.

show abstract

supporting

confidence: 86%

“…As shown in detail by NMR analysis in supporting Figs. S2-S4 and described elsewhere (9,15,16), the alkaline-induced degradation of ampicillin under our experimental conditions can be summarized according to Fig. 1.…”

Section: Electrophysiological Measurementsmentioning

confidence: 99%

Ampicillin permeation across OmpF, the major outer-membrane channel in Escherichia coli

Ghai

Bajaj

Bafna

et al. 2018

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Do Nascimento et al (2013) reported that ABPC remained stable for 2.7 and 11 days in human plasma at 2 and −20°C , respectively. ABPC obtained from porcine muscle exhibits good stability over 8 months at −75°C (Verdon et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Measurement of ampicillin residue levels in chicken eggs during and after medicated feed administration by LC-MS/MS

Hamamoto

Akama

Mizuno

2015

Food Additives & Contaminants: Part A

View full text Add to dashboard Cite

A simple and sensitive LC-MS/MS method was developed and validated for the determination of ampicillin (ABPC) in chicken eggs. Residues were extracted by reverse-phase solid-phase extraction. Chromatographic separation was performed using a reverse-phase column with an elution gradient. The limits of detection and quantification were 0.01 and 0.1 ng g(-1), respectively. For the 0.1-50 ng g(-1) concentration range, mean recovery and accuracy values were 93.9-98.5% and 100.2-118.0%, respectively. ABPC residue concentrations in eggs before, during and after 7 days of medicated feeding of maximum dosage (40 mg kg(-1) body weight day(-1)) of ABPC were determined with the LC-MS/MS method. The maximum concentration of ABPC in eggs was 3.6 ± 1.7 ng g(-1) (mean ± SD) on the last day of the administration period. Residue concentrations of ABPC in eggs during and after ABPC administration were not over the Japanese maximum residue limit of 0.01 mg kg(-1).

show abstract

“…[17] Early diagnosis and quick optimization of the dosing are crucial for the patients survival. In afew hospitals,TDM of antimicrobials for managing sepsis is currently done using HPLC, [20] which is accurate but not accessible to all hospitals.Ampicillin is afirst line antibiotic in treating sepsis and exhibits negligible binding to plasma proteins, [21] but has low stability in biological fluids, [22] making the time between sampling and analysis critical. In afew hospitals,TDM of antimicrobials for managing sepsis is currently done using HPLC, [20] which is accurate but not accessible to all hospitals.Ampicillin is afirst line antibiotic in treating sepsis and exhibits negligible binding to plasma proteins, [21] but has low stability in biological fluids, [22] making the time between sampling and analysis critical.…”

mentioning

confidence: 99%

“…[18] It is one of the major causes of death in newborns, [19] when both sample volume and time are critical. In afew hospitals,TDM of antimicrobials for managing sepsis is currently done using HPLC, [20] which is accurate but not accessible to all hospitals.Ampicillin is afirst line antibiotic in treating sepsis and exhibits negligible binding to plasma proteins, [21] but has low stability in biological fluids, [22] making the time between sampling and analysis critical.…”

mentioning

confidence: 99%

Electrokinetic Size and Mobility Traps for On‐site Therapeutic Drug Monitoring

2015

View full text Add to dashboard Cite

The extraction of target analytes from biological samples is ab ottlenecki na nalysis.Amicrofluidic device featuring an electrokinetic size and mobility trap was formed by two nanojunctions of different pore sizet oe xtract and concentrate analytical targets from complex samples.The trap was seamlessly coupled with electrophoretic separation for quantitative analysis.The device was applied to the analysis of ampicillin levels in blood within 5min and al inear response over the range of 2.5-20 mgmL À1 .T his covers the recommended levels for treating sepsis,acritical condition with 30 to 50 %m ortality and unpredicted drug levels.T he device provides an ew opportunity for on-site therapeutic drug monitoring,w hichs hould enable quick and accurate dosing and may save lives in such critical conditions.

show abstract

Short-term stability studies of ampicillin and cephalexin in aqueous solution and human plasma: Application of least squares method in Arrhenius equation

Cited by 22 publications

References 16 publications

Ampicillin permeation across OmpF, the major outer-membrane channel in Escherichia coli

Ampicillin permeation across OmpF, the major outer-membrane channel in Escherichia coli

Measurement of ampicillin residue levels in chicken eggs during and after medicated feed administration by LC-MS/MS

Electrokinetic Size and Mobility Traps for On‐site Therapeutic Drug Monitoring

Contact Info

Product

Resources

About