Short-term increase of serum troponin I and serum heart-type fatty acid-binding protein (H-FABP) in dogs following administration of formoterol

Abstract

et al.. Short-term increase of serum troponin I and serum heart-type fatty acid-binding protein (H-FABP) in dogs following administration of formoterol. Experimental and Toxicologic Pathology, Elsevier, 2010, 62 (4) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A c c e p t e d m a n u s c r i p t -2 - AbstractIn this paper, changes in serum levels of the cardiac biomarkers troponin I and the heart-type fatty acid-binding protein (H-FABP) following administration of a longacting ȕ 2 -sympathicomimeticum (LABA = long-acting beta-agonist) to dogs were measured.We measured troponin I in dogs in a four week repeated-dose study with inhalative administration of formoterol (13 μg/kg/d) and a glucocorticoid/formoterol combination (143/16 μg/kg/d). The medians of troponin I increased within three days in both groups, far beyond the cut-off level (0.1 μg/L), but returned to baseline levels on study day 9. The increase was more pronounced in the formoterol-only group (3.29 μg/L) compared to the glucocorticoid/formoterol combination group (1.32 μg/L).In a second study, we measured serum troponin I as well as serum H-FABP levels in several samples over seven days in dogs, receiving a single inhalative dose of a glucocorticoid/formoterol combination (120/12 μg/kg/d). The median of the troponin I concentration increased above the cut-off level within two hours, that of H-FABP within four hours. The medians of both parameters were temporarily above the cut-off levels even on study day 7.Both studies were conducted according to national animal welfare guidelines.To our knowledge, this is the first report which shows a corresponding increase of troponin I and H-FABP in dogs treated with formoterol. Both parameters are more sensitive to detect a drug-induced cardiac injury compared to total LDH, total CK as well as CK MB activity. However, it is recommended to take at least three blood samples per day to assess a temporary increase of troponin I.A c c e p t e d m a n u s c r i p t -3 -

The effect of tilmicosin and diclofenac sodium combination on cardiac biomarkers in sheep

“…Cardiac troponins consist of three subunits, cardiac troponin-C (cTn-C), troponin-I (cTn-I) and troponin-T (cTn-T). cTn-I is responsible for the binding of myosin to actin filaments, while cTn-T acts as the bridging protein towards tropomyosin (Strauss et al 2010). CK-MB, an isoenzyme of CK, is especially present in the cardiac tissue and is considered as an indicator of myocyte damage (Yazar et al 2002a).…”