Short-term developmental toxicity and potential mechanisms of the herbicide metamifop to zebrafish (Danio rerio) embryos

Zhao, Feng; Li, Hui; Cao, Fangjie; Chen, Xiangguang; Liang, Yong; Qiu, Lihong

doi:10.1016/j.chemosphere.2019.124590

Cited by 37 publications

(13 citation statements)

References 60 publications

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“…It is known that excessive oxygen radicals are the main culprit of oxidative stress in vivo . SOD and CAT are regarded as important components of antioxidant enzymes in the oxidative stress response.…”

Section: Discussionmentioning

confidence: 99%

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Polystyrene Nanoplastic Exposure Induces Developmental Toxicity by Activating the Oxidative Stress Response and Base Excision Repair Pathway in Zebrafish (Danio rerio)

et al. 2022

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The widespread accumulation of nanoplastics is a growing concern for the environmental and human health. However, studies on the mechanisms of nanoplastic-induced developmental toxicity are still limited. Here, we systematically investigated the potential biological roles of nanoplastic exposure in zebrafish during the early developmental stage. The zebrafish embryos were subjected to exposure to 100 nm polystyrene nanoplastics with different concentrations (0, 100, 200, and 400 mg/L). The results indicated that nanoplastic exposure could decrease the hatching and survival rates of zebrafish embryos. In addition, the developmental toxicity test indicated that nanoplastic exposure exhibits developmental toxicity via the inhibition of the heart rate and body length in zebrafish embryos. Besides, behavioral activity was also significantly suppressed after 96 h of nanoplastic exposure in zebrafish larvae. Further biochemical assays revealed that nanoplastic-induced activation of the oxidative stress responses, including reactive oxygen species accumulation and enhanced superoxide dismutase and catalase activities, might affect developmental toxicity in zebrafish embryos. Furthermore, a quantitative polymerase chain reaction assay demonstrated that the mRNA levels of the base excision repair (BER) pathway-related genes, including lig1 , lig3 , polb , parp1 , pold , fen1 , nthl1 , apex , xrcc1 , and ogg1 , were altered in zebrafish embryos for 24 h after nanoplastic exposure, indicating that the activation of the BER pathway would be stimulated after nanoplastic exposure in zebrafish embryos. Therefore, our findings illustrated that nanoplastics could induce developmental toxicity through activation of the oxidative stress response and BER pathways in zebrafish.

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Section: Discussionmentioning

confidence: 99%

“…54 It is known that excessive oxygen radicals are the main culprit of oxidative stress in vivo. 55 SOD and CAT are regarded as important components of antioxidant enzymes in the oxidative stress response. The dysfunction of ROS and oxidative stress in the cell would lead to severe disorders and diseases.…”

Section: Discussionmentioning

confidence: 99%

Polystyrene Nanoplastic Exposure Induces Developmental Toxicity by Activating the Oxidative Stress Response and Base Excision Repair Pathway in Zebrafish (Danio rerio)

et al. 2022

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“…The overwhelming prooxidant intracellular status upon MPs exposure could also affect metabolic pathways and cell survival via an apoptotic cascade of events through which the intrinsic pathway is activated (Grutter, 2000). Extensive research on aquatic pollution has reported the induction of caspase activity and upregulation of pro-apoptotic and caspase genes in aquatic organisms exposed to xenobiotics, such as MPs (Liu et al, 2007(Liu et al, , 2019Détrée and Gallardo-Escárate, 2017;Sant et al, 2018;Zhao et al, 2019;Shi et al, 2020). Surprisingly, the present results indicated that the effect on caspases 3/7 was significantly inhibited in the digestive gland of S. plana under exposure to any of the MPs treatments (Figure 4), thus signifying an interruption in apoptotic mechanism.…”

Section: Biochemical Biomarkersmentioning

confidence: 99%

Perfluorooctane sulfonic acid (PFOS) adsorbed to polyethylene microplastics: Accumulation and ecotoxicological effects in the clam Scrobicularia plana

Islam

Fonseca

Vilke

et al. 2021

Marine Environmental Research

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“…miR-150 antagomiR significantly downregulated apaf1, p53 and cyto c expression levels compared with antagomir nc. p53 and apaf1 are proapoptotic genes (30). Moreover, apoptosis can be further enhanced when p53 proteins are combined with cyto c (31).…”

Section: Discussionmentioning

confidence: 99%

Promotive role of microRNA‑150 in hippocampal neurons apoptosis in vascular dementia model rats

Wei

Zhang

et al. 2021

Mol Med Rep

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Cognitive impairment is one of the primary features of vascular dementia (VD). However, the specific mechanism underlying the regulation of cognition function in VD is not completely understood. The present study aimed to explore the effects of microRNA (miR)-150 on VD. To determine the effects of miR-150 on cognitive function and hippocampal neurons in VD model rats, rats were subjected to intracerebroventricular injections of miR-150 antagomiR. The Morris water maze test results demonstrated that spatial learning ability was impaired in VD model rats compared with control rats. Moreover, compared with antagomiR negative control (NC), miR-150 antagomiR alleviated cognitive impairment and enhanced memory ability in VD model rats. The triphenyltetrazolium chloride, Nissl staining and immunohistochemistry results further demonstrated that miR-150 knockdown improved the activity of hippocampal neurons in VD model rats compared with the antagomiR NC group. To validate the role of miR-150 in neurons in vitro , the PC12 cell line was used. The flow cytometry and Hoechst 33342/PI double staining results indicated that miR-150 overexpression significantly increased cell apoptosis compared with the mimic NC group. Moreover, the dual-luciferase reporter gene assay results indicated that miR-150 targeted HOXA1 and negatively regulated HOXA1 expression. Therefore, the present study indicated that miR-150 knockdown ameliorated VD symptoms by upregulating HOXA1 expression in vivo and in vitro .

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Short-term developmental toxicity and potential mechanisms of the herbicide metamifop to zebrafish (Danio rerio) embryos

Cited by 37 publications

References 60 publications

Polystyrene Nanoplastic Exposure Induces Developmental Toxicity by Activating the Oxidative Stress Response and Base Excision Repair Pathway in Zebrafish (Danio rerio)

Polystyrene Nanoplastic Exposure Induces Developmental Toxicity by Activating the Oxidative Stress Response and Base Excision Repair Pathway in Zebrafish (Danio rerio)

Perfluorooctane sulfonic acid (PFOS) adsorbed to polyethylene microplastics: Accumulation and ecotoxicological effects in the clam Scrobicularia plana

Promotive role of microRNA‑150 in hippocampal neurons apoptosis in vascular dementia model rats

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