Promotive role of microRNA‑150 in hippocampal neurons apoptosis in vascular dementia model rats

Abstract

Cognitive impairment is one of the primary features of vascular dementia (VD). However, the specific mechanism underlying the regulation of cognition function in VD is not completely understood. The present study aimed to explore the effects of microRNA (miR)-150 on VD. To determine the effects of miR-150 on cognitive function and hippocampal neurons in VD model rats, rats were subjected to intracerebroventricular injections of miR-150 antagomiR. The Morris water maze test results demonstrated that spatial learning ability was impaired in VD model rats compared with control rats. Moreover, compared with antagomiR negative control (NC), miR-150 antagomiR alleviated cognitive impairment and enhanced memory ability in VD model rats. The triphenyltetrazolium chloride, Nissl staining and immunohistochemistry results further demonstrated that miR-150 knockdown improved the activity of hippocampal neurons in VD model rats compared with the antagomiR NC group. To validate the role of miR-150 in neurons in vitro , the PC12 cell line was used. The flow cytometry and Hoechst 33342/PI double staining results indicated that miR-150 overexpression significantly increased cell apoptosis compared with the mimic NC group. Moreover, the dual-luciferase reporter gene assay results indicated that miR-150 targeted HOXA1 and negatively regulated HOXA1 expression. Therefore, the present study indicated that miR-150 knockdown ameliorated VD symptoms by upregulating HOXA1 expression in vivo and in vitro .

Treadmill exercise pretreatment ameliorated structural synaptic plasticity impairments of medial prefrontal cortex in vascular dementia rat and improved recognition memory

“…Therefore, the neuronal impairment will likely cause changes in structural synaptic plasticity. Previous studies demonstrated via Nissl staining that VD rats displayed significantly neuron structural changes in the hippocampal 45 . The immunostaining results in our study demonstrated that treadmill exercise pretreatment can ameliorate VD-induced the decrease of neuron density in the medial prefrontal cortex region of VD rats, as determined by NeuN immunostaining.…”

Acupuncture attenuates cognitive impairments in vascular dementia through inhibiting miR-143-3p

“…miR-153 is elevated in the plasma of dementia patients and rats subjected to 2VO surgery and may serve as a drug target for VD (Yan et al, 2020). Wei and others (Wei et al, 2021) have shown that knockdown of miR-150 improves VD symptoms via regulating the expression of homeobox A1 (HOXA1) in vivo and in vitro. In addition, abnormal miR-143-3p expression is related to ischemic brain injury (Zhou et al, 2021) and acute ischemic stroke (Tiedt et al, 2017).…”

MicroRNA‐150‐5p is upregulated in the brain microvasculature during prenatal alcohol exposure and inhibits the angiogenic factor Vezf1

“…miR‐150‐5p was also shown to increase blood brain barrier permeability in response to CAO by targeting Tie2 (Fang et al, 2016). In a mouse model of vascular dementia, miR‐150 was upregulated in hippocampal neurons and responsible for neuronal apoptosis by negatively regulating HOXA1 expression (Wei et al, 2021). Although studies of miR‐150‐5p within the CNS indicate its role to be a negative regulator of angiogenesis, some studies of miR‐150‐5p in the periphery indicate the opposite function and increase migration and proliferation (Luo et al, 2018; Zhang et al, 2010).…”