Short Communication: Screening the potency of Zingiberaceae leaves as antioxidant and antiaging agent

Abstract

Abstract. Zahra U, Kartika Y, Batubara I, Darusman LK, Maddu A. 2016. Short Communication: Screening the potency of . Zingiberaceae is a family of plant with antioxidant, anticancer, and antiaging activities. The use of the leaves part of this family has never been explored. Therefore, the aim of this research is to evaluate the antioxidant and antiaging activities of some Zingiberaceae's leaves. The species of Zingiberaceae used in this study are Alpinia galanga (L), Curcuma aeruginosa Roxb, Curcuma longa L, Curcuma zedoaria, Curcuma xanthorrhiza Roxb, Boesenbergia rotunda (L.), Elettaria cardamomum, Zingiber zerumbet, Zingiber officinale Roscoe, and Zingiber purpureum Roscoe. Leaves were extracted with 2 different solvents sequentially: ethyl acetate and methanol. Total phenolic and flavonoid contents of the extracts were determined spectrophotometrically. Antioxidant activities were tested by using DPPH (2,2-diphenyl-1-picrylhydrazyl ) and ABTS (2,2'-azino-bis-3-ethylbenzothiazolin-6-sulfonic acid), whilst antiaging activity was tested by using antiglycation method. The results showed that the flavonoid content of the extracts were in the range of 0.83-25.17 g QE/100 g extract with total phenolic content ranging from 9.80-91.71 GAE mg/100 mg extract. The methanol extract of Curcuma xanthorrhiza displayed the highest antioxidant capacity in the DDPH (IC 50 282.35 mg/mL) and TEAC (10.08%) assays, followed by the ethyl acetate extract of C. longa, ethyl acetic extract of C. xanthorrhiza and methanol extract of Z. officinale, while the most active antiglycation is the methanol extract of Z. officinale (IC 50 203.85 mg/L).

Antibacterial and Biofilm Degradation Activity of Extract From Steam Distillation Residue of Zingiberaceae Leaves Against Streptococcus mutans

“…The activity of this extract was not as good as the positive control chlorhexidine. C. zedoaria leaves methanol extract as has been reported by Zahra et al (2016) has the activity as an antioxidant with IC50 of DDPH 544.05 mg/L and 7.0 mg Trolox Equivalents Antioxidant Capacity/g extract [6]. It means the C. zedoaria leaves before distillation process has antioxidant activity and its distillation residue has activity to degrade the biofilm.…”

“…The highest total phenolic content was found in the residue of Z. officinale leaves of methanol extract while the lowest was found in the residue of C. zedoaria leaves of nhexane extract. It must be noted that the total phenolic content of extract of distillation residue of Zingiberaceae leaves is lower than the fresh extract [6]. It means that the distillation process reduces the total phenolic content in the sample.…”

“…Qualitative analysis of tannin, saponin and terpenoids were done according to the methods described by Edeoga [4] and that by Ayoola et al [5] for flavonoid and alkaloid. Total phenolic content was determined by spectrometry according to the previous method [6]. Briefly, deionized water and Folin-Ciocalteu phenol reagent were added to the extract and the control sample of gallic acid which was followed by the addition of sodium carbonate 7% after 5 minutes of incubation.…”

Antioxidant, Antiglycation, and Antiaging Activity of Roselle Petals Extract (Hibiscus sabdariffa L.)

“…Low purity means that other unwanted materials, such as components from solvent, may exist in the extract, thereby producing less powerful activity. [13]. Antiglycation activity of extracts isolated from plants was due to presence of high content of polyphenol.…”

Relationship between Zingiberaceae Leaves Compounds and its Tyrosinase Activity

“…make the leaves possible to be used as a new natural source for skin lightening agent. Some research related to Zingiberaceae leaves had been conducted such as Zingiberaceae leaves essential oils as antimicrobe and biofilm degradation (Batubara et al, 2016), and Zingiberaceae leaves as antioxidant and antiaging agent (Zahra et al, 2016), while the potency of Zingiberaceae leaves as tyrosinase inhibitor had not been developed yet. In addition to the potency as skin whitening, the correlation the compounds from Zingiberaceae leaves and the activity also need to be understand.…”