Antioxidant, Antiglycation, and Antiaging Activity of Roselle Petals Extract (Hibiscus sabdariffa L.)

Abstract

Accumulation of radicals may lead to oxidative stresses, which is the main cause of aging. Antioxidant and antiglycation agents can protect the cells from oxidative stresses. An antioxidant source can be obtained from various sources, including plants. In this study we aimed to analyze the potential properties of roselle (Hibiscus sabdariffa. L) petals extract as antioxidant agent both via in vitro and in vivo assays. The roselle petals were extracted with 70% ethanol as solvent. The IC50 value of antiglycation activity of the extract was 527,9 ppm. The antiaging and antioxidant effects of roselle petals extract were analyzed in vivo, by using model organisms Saccaharomyces cerevisiae via spot assay. Following administration of roselle extract at concentration of 150 ppm, 300 ppm, 450 ppm, 600 ppm, 750 ppm, the viability of yeast cells at day-5 was higher than that the untreated group (0 ppm). In addition, yeast was found more tolerant toward H2O2-induced oxidative stress conditions as treated with the roselle extract (300 ppm), particularly in the stationary phase. To our knowledge, this is the frist study to report the anti-aging and antioxidant properties of roselle petals by using yeast S. cerevisiae as model organism.