SHORT COMMUNICATION: Neutrophil oxidative metabolism and haemogram of sheep experimentally infected with Haemonchus contortus and supplemented with selenium and vitamin E

Abstract

The objective of this study was to evaluate neutrophil oxidative metabolism and haemogram in sheep experimentally infected with Haemonchus contortus and supplemented with selenium and vitamin E. Twenty male Corriedale sheep were utilised and distributed into four experimental groups each with five animals infected with larvae: G1--supplemented with sodium selenite, 0.2 mg/kg body weight (bw) given intramuscularly (IM); G2--supplemented with sodium selenite and vitamin E, 0.2 mg/kg bw and 2000 IU per animal, respectively, both IM; G3--supplemented with vitamin E, 2000 IU per animal IM; G4--not supplemented. A haemogram and the number of parasite eggs were determined in samples of blood and faeces, respectively, on days zero (T0), 20 (T1), 40 (T2) and 60 (T3) and nitroblue tetrazolium (NBT) assays were performed on heparinized blood samples taken on days zero, 30 and 60. A lower total leucocyte count was detected in G1 in relation to G4 at T4. Lymphocytes were reduced in G1 in relation to G3 and G4 at T3. In both non-stimulated (NBT-NS) and stimulated (NBT-S) dye reduction assays, there was reduced activity at 60 days, in relation to other times in the groups treated with selenium (G1 and G2). On the basis of results obtained, we conclude that supplementation with selenium provides better antioxidant protection to neutrophils.

Correlation of Trace Mineral Profiles with Gastrointestinal Worm Burden in Rangeland Sheep of Chakwal District, Punjab, Pakistan

“…Vellema et al (1996) documented higher EPG in lambs offered low level of Co diet compared to Co supplemented counterpart. The results reported by Camargo et al (2010) and Celi et al (2010) are also not in line with those observed in the present study as they have noted a significant EPG reduction in Se medicated animals. Similarly, application of Mo significantly reduced H. contortus burden in sheep (Suttle et al, 1992).…”

Sodium Selenite Protects Against Silver Nanoparticle-Induced Testicular Toxicity and Inflammation

“…MDA content manifests the level of lipid peroxidation and indirectly represents the level of damage of the cell and tissue [37]. Results of the present study showed that the amount of MDA was high in AgNP-treated rats which was supported by the previous studies [38,39]. The elevated level of MDA might be due to enhanced formation of free radicals.…”

A Summary of New Findings on the Biological Effects of Selenium in Selected Animal Species—A Critical Review

“…Selenium (Se) is an essential trace element [ 1 , 2 ] that evinces antioxidant activity [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ], anti-inflammatory [ 2 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ], antimutagenic [ 26 , 27 ], anticarcinogenic [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ] or chemopreventive [ 2 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ], antiviral [ 2 ], antibacterial [ 45 , 46 , 47 , 48 ], antifungal [ 49 , 50 ] and antiparasitic effects [ 51 ,…”