Short Communication: Diversity of cellulolytic bacteria isolated from coastal mangrove sediment in Logending Beach, Kebumen, Indonesia

Pramono, Hendro; Mariana, Afifah; Ryandini, Dini; Sudiana, Eming

doi:10.13057/biodiv/d220433

Cited by 6 publications

(5 citation statements)

References 20 publications

(24 reference statements)

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“…Genus Celullomonas ini dapat ditemukan di tumpukan tanaman yang membusuk pada tanah (Holt et al, 1994). Menurut Pramono et al (2021) pada uji sifat fisiologis Cellulomonas urease negatif, motil namun ada juga yang non-motil, uji gelatin positif, selulosa positif, pati positif dan uji manitol negatif.…”

Section: Cellulomonasunclassified

Screening and Determination of Potential Cellulolytic Bacteria from Mangrove Ecosystem

Batubara

Suparjo

Maritsa

et al. 2022

Jurnal Perikanan dan Kelautan

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The mangrove ecosystem is a coastal wetland resource that has a diversity of biodiversities such as flora, fauna, and microorganisms. Microbes are an important component in the mangrove environment not only play a role in creating and maintaining boosters but also work as a source of biotechnology products. Cellulolytic bacteria are a group of cellulase-producing bacteria capable of breaking down cellulose into glucose monomers, and cellulose as a source of carbon and energy. This study aims to screen and determine the diversity of cellulolytic bacteria from the mangrove ecosystem. The research method was experimentally by exploring the diversity of cellulolytic bacteria from soil and sediment from the mangrove ecosystem. Three sampling locations were selected, such as litter soil, soil in the root area, and soil in the tidal area. Screening of potential bacteria producing cellulase enzymes was carried out by growing bacteria on Carboxy Methyl Cellulose (CMC) agar medium which was cultured for 48 hours at 37°C. The bacteria that produced the greatest cellulolytic activity were characterized by their morphological and physiological characteristics. The results of bacterial characterization were then adjusted according to Bergey's Manual of Determinative Bacteriology. Based on the analysis showed three bacterial isolates were obtained that had the largest cellulolytic activity index, namely MS06 (9.73), MS08 (5.41), and MS02 (5.07). The results showed that three isolates had the same characteristics as the bacterial genera Bacillus, Cellulomonas, and Micrococcus.

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Section: Cellulomonasunclassified

Screening and Determination of Potential Cellulolytic Bacteria from Mangrove Ecosystem

Batubara

Suparjo

Maritsa

et al. 2022

Jurnal Perikanan dan Kelautan

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“…Isolat bakteri LG8, LG13, LG20, LG21, LG37, LG45, LG50, LG85, LG108 dan LG113 telah diisolasi dari sedimen mangrove pantai Logending (Pramono et al, 2021) dan belum diketahui kemampuan amilolitiknya. Tujuan dari penelitian ini yaitu mengetahui kemampuan isolat bakteri sedimen mangrove pantai Logending dalam menghasilkan enzim amilase, mengetahui suhu dan pH optimum aktivitas amilase, dan mengetahui identitas isolat bakteri amilolitik asal sedimen mangrove pantai Logending.…”

Section: Pendahuluanunclassified

Untitled

Rahmawati¹,

Oedjijono²,

Ryandini³

2022

bioe

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Penelitian ini bertujuan untuk mengetahui kemampuan isolat bakteri sedimen mangrove dalam menghasilkan enzim amilase, mengetahui pH dan suhu optimum aktivitas amilase yang dihasilkan, dan mengidentifikasibakteri penghasil amilase. Penelitian dilakukan dengan metode survei. Tahap penelitian meliputi, skrining bakteri amilolitik, pembuatan kurva tumbuh, produksi amilase, optimasi aktivitas amilolitik pada variasi suhu (35ºC; 36ºC; 37ºC; 38ºC) dan pH (4,5; 5; 5,5; 6) serta karakterisasi isolat amilolitik. Parameter yang diukur yaitu indeks amilolitik, unit aktivitas enzim amilase, total unit bakteri, dan identitas bakteri amilolitik asal sedimen mangrove. Analisis data dilakukan secara deskriptif. Hasil pengukuran indeks amilolitik tertinggi ditunjukkan oleh isolat LG113 dengan nilai indeks amilolitik 9,86 mm dari 10 isolat amilolitik asal sedimen mangrove. Suhu optimum aktivitas amilase adalah 37ºC yaitu sebesar 2,13 U/mL dan pH optimum adalah 6 yaitu sebesar 2,14 U/mL. Jumlah total sel bakteri amilolitik padaakhir masa produksi adalah 1,94x1013 CFU/mL. Identitas isolat bakteri amilolitik asal sedimen mangrove pantai Logending termasuk anggota genus Bacillus.

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“…With this condition, the mangrove ecosystem has a high diversity of microorganisms. Several studies have explored the diversity and potential of bacteria in intertidal ecosystems, for example, cellulolytic bacteria from mangrove sediment (Kurniawan et al 2017;Ambeng et al 2019;Nursyirwani et al 2020;Pramono et al 2021), mangrove vegetation (Hwanhlem et al 2013;Hastuti et al 2015), and surrounding ecosystems (Elayaraja et al 2014;Maharsiwi et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Bacteriocinogenic Lactic Acid Bacteria Isolated from Mangrove Sediment in Indonesia: Growth Optimization, Bacteriocin Production, and its Application in Food Preservation

Kusharyati,

Oedjijono,

Satwika

et al. 2023

HAYATI J Biosci

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The mangrove ecosystem is unique because it is located between marine and land. Little research is exploring lactic acid bacteria (LAB) from mangrove ecosystems. The LAB LG71 isolate is successfully isolated from the sediment of mangrove ecosystems on the coast of Logending, Jawa Tengah (Indonesia). In this study, we aimed to know the effect of glucose supplementation on the growth of LG71 isolate, bacteriocin production, and its application in food preservation. The characterization results showed that the LG71 isolate is Lactobacillus sp. Interestingly, the LG71 isolate is catalase-positive since this character is rarely found in the LAB group. Supplementing 0.25% glucose to MRSB medium and an incubation time of 15 hours is the best treatment for producing Lactobacillus LG71 isolate cell biomass. A 2% concentration of crude extract of Lactobacillus LG71 bacteriocins is the best concentration against Salmonella typhi both during in vitro and in vivo tests in fish balls. The addition of glucose affects the production of Lactobacillus LG71 cell biomass, and the bacteriocin derived from Lactobacillus LG71 gives increased protection against S. typhi and offers an alternative for food preservation.

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Short Communication: Diversity of cellulolytic bacteria isolated from coastal mangrove sediment in Logending Beach, Kebumen, Indonesia

Cited by 6 publications

References 20 publications

Screening and Determination of Potential Cellulolytic Bacteria from Mangrove Ecosystem

Screening and Determination of Potential Cellulolytic Bacteria from Mangrove Ecosystem

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Bacteriocinogenic Lactic Acid Bacteria Isolated from Mangrove Sediment in Indonesia: Growth Optimization, Bacteriocin Production, and its Application in Food Preservation

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