Short Communication: Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah (Indonesia) using ISSR marker

Abstract

Abstract. Handayani F, Rahayu SP. 2017. Assessment of genetic diversity in Lai (Durio kutejensis) local cultivars of Batuah . Lai is an indigenous germplasm of Kalimantan which has potency to be developed as superior tropical fruit. Lai has attractive flesh color from yellow to reddish orange which represents the high content of carotene with smooth aroma or odorless fruit, and fruit storage period which longer than durian. Unfortunately, nowadays there is limited scientific information about its genetic diversity as important basic information for genetic conservation and breeding program. Batuah village in Kutai Kartanegara District is one of the center of origin and center of diversity of Lai in East Kalimantan, Indonesia which has many local cultivars with superior traits. The aim of this research was to study the genetic diversity of eight Lai local cultivars of Batuah village using molecular marker of ISSR. The total genomic DNA of eight Lai cultivars was isolated and was then used as template in the PCR amplification reaction using 10 ISSR primers. The result shows that similarity coefficient of eight Lai cultivars ranged between 0,34 and 0,58. Seven cultivars were grouped in the same cluster with 0,44 of similarity coefficient, while Lai Durian was separated in 0,34 of similarity coefficient. Genetically, the most similar existing cultivars are Lai Kuning and Lai Belimbing with the similarity coefficient of 0,58.

Floral biology and pollination strategy of Durio (Malvaceae) in Sarawak, Malaysian Borneo

“…The fruit drops unopened. The seeds are ellipsoid, glossy brown, completely enclosed by a fleshy, yellow, nice smelling, and palatable aril (Kostermans 1958;Handayani 2016;Santoso 2010;Handayani and Rahayu 2017;Tirtawinata and Mulyanto 2017).…”

Genetic variability of lemon basil (Ocimum × africanum Lour.) from Indonesian archipelago based on morphological and inter-simple sequence repeats markers

“…Data generated from ISSR marker can be used for genetic selection in the context of the maintenance and improvement of genetic diversity that can support plant breeding programs (Lal et al 2012). ISSR has been used to determine various plants such as Daemonoropsdraco (Asra et al 2014) and Duriokutejensis (Handayani and Rahayu 2017). This study was aimed to describe the genetic diversity of O.…”