The diversity of superior Indonesian durians based on molecular markers

Angeliena, Anggi; Ma'ruf, Anang; Sidiq, Husni Ahmad; Anggraito, Yustinus Ulung; Habibah, Noor Aini; Huyop, Fahrul; Retnoningsih, Amin

doi:10.1063/1.5125547

Cited by 6 publications

(10 citation statements)

References 12 publications

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“…The difference in the number of alleles found presumably happened due to differences in geographic areas that affect the genetic diversity of durians. Allele polymorphism in this study was higher than Thailand durian Vanijajiva (2012), but lower than Kundur Island durian (Angeliena et al 2019). The higher number of polymorphic alleles in Brongkol durian indicates that the durian in Nonthaburi Province has more limited genetic diversity than the superior durian in Brongkol.…”

Section: Brongkol's Superior Durian Polymorphismcontrasting

confidence: 55%

“…The higher number of polymorphic alleles in Brongkol durian indicates that the durian in Nonthaburi Province has more limited genetic diversity than the superior durian in Brongkol. The ISSR4 and ISSR5 primers produced almost the same number of alleles as the Vanijajiva analysis ( 2012), the same as PKBT7, whose results were similar to Angeliena et al (2019).…”

Section: Brongkol's Superior Durian Polymorphismsupporting

confidence: 54%

“…One of the molecular markers often used to study genetic diversity is inter-simple sequence repeat (ISSR) (Vanijajiva 2012;Riupassa et al 2015;Solikin et al 2017;Siew et al 2018;Angeliena et al 2019). This marker is part of a microsatellite sequence that does not code for protein (non-coding region) and comprises one, two, or three nucleotides (Ng and Tan 2015).…”

Section: Introductionmentioning

confidence: 99%

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Molecular based genetic diversity of Brongkol's superior durian germplasm of Semarang, Indonesia

Prakoso

Retnoningsih

2021

Biodiversitas

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Abstract. Prakoso CNY, Retnoningsih A. 2021. Molecular based genetic diversity of Brongkol's superior durian germplasm of Semarang, Indonesia. Biodiversitas 22: 5311-5316. Durio zibethinus is an Indonesian most popular edible durian. This species' genetic diversity is the highest compared to other durian species. Brongkol Village, Semarang, Indonesia is one of the villages that produce superior durian varieties. Brongkol durian is well-known for its sweet and bitter taste of aril with its white to yellow color. This research aimed to analyze the diversity and to identify superior durian in Brongkol using ISSR molecular markers. DNA was isolated from the leaves of 25 varieties of Brongkol superior durian. The DN A was isolated using a modified CTAB method. The genomic DNA was amplified using PCR employing 7 ISSR markers. The amplified DNA band was visualized using a 2% agarose gel electrophoresis. Data were analyzed using the NTSYS’ pc version 2.02 program. The alleles produced in this study were 340-3,000 bp in size. The Polymorphic alleles were found as much as 90.2%. The results of study showed a similarity level of 0.29-0.95. All of the Brongkol superior durians were identified as different varieties. All of the superior Brongkol durians studied were identified as distinct varieties. The results of this study indicated a high diversity of superior durian in Brongkol. In addition, specific alleles were found in varieties B5 and B16.

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Section: Brongkol's Superior Durian Polymorphismcontrasting

confidence: 55%

Section: Brongkol's Superior Durian Polymorphismsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

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Molecular based genetic diversity of Brongkol's superior durian germplasm of Semarang, Indonesia

Prakoso

Retnoningsih

2021

Biodiversitas

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“…The genetic diversity of durian in Indonesia is getting higher with the increasing number of varieties analyzed. Fifty-five varieties of durian Kundur Island, Riau Archipelago, were analyzed using 11 ISSR primers to produce 93.25% allele polymorphism (Angeliena et al 2019). The high genetic diversity of durian in Indonesia is similar to the genetic diversity of other fruits in Indonesia, and the more accessions analyzed, the more alleles produced (Retnoningsih et al 2011).…”

Section: Polymorphism Of Issr Superior Durian Gunungpati Allelementioning

confidence: 99%

“…Therefore, the distribution and cultivation of D. zibethinus in Indonesia are intensive, including in Java. Durio zibethinus is the only durian species with many varieties as genetic diversity within the species (Hikmah et al 2016;Angeliena et al 2019;Habibah et al 2019;Songnuan et al 2019;Hannum et al 2020;Yursak et al 2020;Maranatha et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia

Nasrika

Retnoningsih

2021

Biodiversitas

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Abstract. Nasrika E, Retnonongsih A. 2021. Inter Simple Sequence Repeat molecular markers to reveal the genetic diversity of superior durian of Gunungpati, Semarang, Indonesia. Biodiversitas 22: 4054-4059. Kalimantan is the center of many tropical fruits, including durian. One of the edible durians and favorited by many is Durio zibethinus, which has many superior varieties. Almost all regions in Indonesia have superior durian varieties; for example, superior durian Gunungpati from Gunungpati Sub-district, the center for producing durian in Semarang City, Central Java, Indonesia. ISSR analysis was carried out to reveal the genetic diversity of the superior durian Gunungpati. DNA isolation of 16 superior durians used the modified CTAB method. The genomic DNA was amplified using 10 ISSR primers and then electrophoresed using 2% agarose gel. Data were analyzed using NTSYS PC version 2.02. The resulting allele has a relative size of 220 bp-1800 bp, with 87.9% are polymorphic. The similarity coefficient of 16 varieties was ranged between 0.54-0.88. All the superior durian varieties examined were different accessions so that each variety has the potential to be registered as a new variety of superior Indonesian durian. Specific alleles are found in G1, G3, G7, G8, and G13, which can be an identity of these varieties.

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