Short cigarette smoke exposure facilitates sensitisation and asthma development in mice

Abstract

Epidemiological studies indicate that cigarette smoke exposure is a risk factor for increased sensitisation and asthma development. The aim of this study was to examine the impact of cigarette smoke on sensitisation and allergic airway inflammation in response to a low dose of house dust mite (HDM), and to obtain potential mechanistic insights.Mice were exposed to low doses of HDM extract combined with air or cigarette smoke exposure, either during allergen sensitisation or during the development of allergic airway disease.Mice concomitantly exposed to low-dose HDM, combined with cigarette smoke for 3 weeks, demonstrated an asthmatic phenotype with significantly increased airway eosinophilia, goblet cell metaplasia, airway hyperresponsiveness and a rise in HDM-specific serum immunoglobulin G1, compared to sole HDM or cigarette smoke exposure. In addition, short cigarette smoke inhalation, during the initial contact with HDM allergens, was sufficient to facilitate sensitisation and development of a complete asthmatic phenotype after rechallenge with HDM. Mechanistically, short cigarette smoke exposure amplified dendritic cell-mediated transport of fluorescein isothiocyanate-labelled HDM allergens to the intrathoracic lymph nodes and generated a local T-helper cell type 2 response.Short cigarette smoke exposure is sufficient to facilitate allergic sensitisation and the development of low-dose HDM-induced allergic asthma, possibly by affecting dendritic cell function.

Active and Passive Smoking and the Incidence of Asthma in the Black Women’s Health Study

“…Smoking has profound effects on the airway epithelium, as indicated by smokinginduced alterations of mRNA and miRNA expression (24,27). By effects on epithelial permeability (23,28) and other aspects of epithelial regulation of inflammation (21), tobacco smoke exposure increases allergic sensitization in animal models of inhaled allergen exposure (29,30). Human studies have shown that acute exposure to environmental tobacco smoke worsens allergic airway responses to simultaneously inhaled aeroallergens (31).…”

Epitope Mapping of Rhi o 1 and Generation of a Hypoallergenic Variant

“…H&E and PAS staining of lung histology were done to quantify the eosinophilic infiltrates and mucous gland hyperplasia, respectively, as described (49). For raising antisera, three BALB/c mice (10 g of antigens/mouse) and one New Zealand White rabbit (200 g of antigens/rabbit) were immunized with either rRhi o 1 or TYKW-mut adsorbed to Freund's adjuvants following protocols described previously (50).…”

Cigarette smoke extract-induced interleukin-6 expression is regulated by phospholipase D1 in human bronchial epithelial cells

“…Moreover, secondhand smoke has been linked to asthma (Tamimi et al, 2012;Lanckacker et al, 2013). In addition to those noncancer diseases, cigarette smoking is associated with human cancer development in lung, oral cavity, stomach, pancreas, liver, kidney, urinary bladder, uterine cervix, and bone marrow (Chen et al, 2011a).…”