Shoot multiplication and adventitious regeneration in Sorbus aucuparia

Lall, Sonia; Mandegaran, Z.; Roberts, A. V.

doi:10.1007/s11240-005-9045-3

Cited by 15 publications

(13 citation statements)

References 12 publications

(13 reference statements)

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“…Although the phenomenon of ectopic shoot development on tomato leaves has been mentioned already more than one and a half centuries ago (Duchartre, ) so far nobody had characterized it in detail. Several other compound leaved angiosperms, like Sorbus aucuparia (Lall et al ., ) and Fraxinus excelsior (Hammatt, ), develop adventitious shoots from the base of leaflets. These findings seem to strengthen the similarity between compound leaves and shoots.…”

Section: Discussionmentioning

confidence: 99%

Lateral suppressor and Goblet act in hierarchical order to regulate ectopic meristem formation at the base of tomato leaflets

et al. 2015

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SUMMARYIn seed plants, new axes of growth are established by the formation of meristems, groups of pluripotent cells that maintain themselves and initiate the formation of lateral organs. After embryonic development, secondary shoot meristems form in the boundary zones between the shoot apical meristem and leaf primordia, the leaf axils. In addition, many plant species develop ectopic meristems at different positions of the plant body. In the compound tomato leaf, ectopic meristems can initiate at the base of leaflets, which are delimited by two distinct boundary zones, referred to as the proximal (PLB) and distal (DLB) leaflet boundaries. We demonstrate that the two leaflet boundaries differ from each other and that ectopic meristem formation is strictly limited to the DLB. Our data suggest that the DLB harbours a group of pluripotent cells that seems to be the launching pad for meristem formation. Initiation of these meristems is dependent on the activities of the transcriptional regulators Goblet (Gob) and Lateral suppressor (Ls), specifically expressed in the DLB. Gob and Ls act in hierarchical order, because Ls transcript accumulation is dependent on Gob activity, but not vice versa. Ectopic meristem formation at the DLB is also observed in other seed plants, like Cardamine pratensis, indicating that it is part of a widespread developmental program. Ectopic meristem formation leads to an increase in the number of buds, enhances the capacity for survival and opens the route to vegetative propagation.

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Section: Discussionmentioning

confidence: 99%

Lateral suppressor and Goblet act in hierarchical order to regulate ectopic meristem formation at the base of tomato leaflets

et al. 2015

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“…It has been shown that plant cells have a higher ability to metabolize and transfer nitrogen from organic rather than inorganic sources [29]. Casein hydrolysate has been reported to be effective in various plant developmental processes including somatic embryogenesis [30,31], seed germination and seedling growth [32], and callus proliferation [33]. Additionally, the positive role of casein hydrolysate, as a reduced nitrogen form in vitro shoot regeneration of many plant species has been documented previously [34][35][36].…”

Section: Discussionmentioning

confidence: 97%

“…It has been effective in induction, maturation, and germination of somatic embryos in a number of plant species [39,40]. Glutamic acid is the precursor of L-glutamine, which has been frequently reported to have a stimulatory impact on in vitro plant growth, shoot proliferation [38,39], and somatic embryogenesis [31]. L-glutamine and glutamic acid are directly involved in NH 4 assimilation in plants.…”

Section: Discussionmentioning

confidence: 99%

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

Samiei

Pahnehkolayi

Tehranifar

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Rosa canina is one of the most popular rose species which is widely used as the rootstock for the propagation of rose cultivars. The purpose of the present study is to improve the in vitro propagation efficiency of this valuable plant species using various growth stimulants in a proliferation medium. In this study, in vitro-derived axillary buds of R. canina were inoculated in Vander Salm (VS) medium supplemented with varying levels of organic or inorganic elicitors including casein hydrolysate (200, 400, and 600 mg/l), glutamic acid (2, 4, 8, and 12 mg/l), proline (500, 1000, 1500, and 2000 mg/l), and silver nitrate (25, 50, 75, and 100 mg/l), separately. Benzyl amino purine (BAP) as well as naphthalin acetic acid (NAA) were added to all media at a constant rate to promote shoot proliferation. Results The results indicated that the supplementation of casein hydrolysate to the VS medium markedly stimulated shoot regeneration by 173% in comparison to control. Shoot proliferation was also positively influenced by glutamic acid at all levels, however, at a lesser extent compared to casein hydrolysate. Silver nitrate at 100 mg/l induced the longest shoots (2.52 ± 0.248 cm) and maximum leaf number (8.90 ± 0.276) among all treatments. Although it did not encourage efficient shoot regeneration, the highest quality shoots with maximum growth vigor were observed in this treatment. Conclusion In this study, the promising role of casein hydrolysate in combination with plant growth regulators has been emphasized for the improved efficiency of R. canina regeneration protocol. Moreover, the addition of silver nitrate to the culture medium seems vital for enhancing the quality of regenerated shoots. The results of this study could be beneficial either for the further pharmaceutical or biochemical investigations of R. canina or commercial purposes for mass propagation of this specimen.

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“…A micropropagation protocol was first developed for economically important Sorbus species such as S. torminalis [ 28 , 29 , 30 ], S. aucuparia [ 29 , 31 , 32 , 33 ] and S. domestica [ 28 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ]. These species are mainly used for wood production, as well as in the food industry and in ornamental horticulture.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation as a Tool for the Conservation of Autochthonous Sorbus Species of Czechia

Šedivá

Velebil

Zahradník

2023

Plants

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Members of the genus Sorbus are the only endemic tree species that occur in Czechia. They are important components of endangered plant communities. Their natural regeneration is usually problematic because of their mode of reproduction and because they can survive in rare populations with small numbers of individuals. The aim of this study was to develop a successful micropropagation protocol for selected Sorbus species, of which two are endemic (S. gemella and S. omissa) and two are hybrid (S. × abscondita and S. × kitaibeliana). We found significant differences in shoot induction and rooting ability between the Sorbus species under study. With the exception of S. × abscondita, N6-benzyladenine had a significantly greater effect on shoot regeneration, both in terms of shoot number and total shoot length, than meta-topolin. Root induction was key to the successful micropropagation of the Sorbus species studied. Our results show that four Sorbus species can be successfully rooted under ex vitro conditions, without a rooting powder treatment in a steamed peat-perlite substrate. Auxin-untreated microcuttings of S. gemella, S. × kitaibeliana and S. omissa, but not S. × abscondita, rooted better than ones treated with indole-3-butyric acid. This is the first time a micropropagation protocol for S. omissa, S. × abscondita and S. × kitaibeliana has been published.

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Shoot multiplication and adventitious regeneration in Sorbus aucuparia

Cited by 15 publications

References 12 publications

Lateral suppressor and Goblet act in hierarchical order to regulate ectopic meristem formation at the base of tomato leaflets

Lateral suppressor and Goblet act in hierarchical order to regulate ectopic meristem formation at the base of tomato leaflets

Organic and inorganic elicitors enhance in vitro regeneration of Rosa canina

Micropropagation as a Tool for the Conservation of Autochthonous Sorbus Species of Czechia

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