Shoot induction and organogenesis in vitro: A developmental perspective

Hicks, G. S.

doi:10.1007/bf02632113

Cited by 74 publications

(47 citation statements)

References 78 publications

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“…Both roots and cotyledons of young seedlings actively produce PGRs involved in the control of organogenesis (Hicks, 1994). Pozueta-Romero, Houlne, Canas, Schantz, and Chamarro (2001) reported the successful regeneration of tomato and bell peppers on medium without exogenous growth hormones from decapitated explants with radicle, hypocotyl and with or without cotyledon explants.…”

Section: Discussionmentioning

confidence: 99%

Effect of Cultivar and Explants Type on Tissue Culture Regeneration of Three Nigerian Cultivars of Tomatoes

Ajenifujah-Solebo¹,

Isu²,

Olorode³

et al. 2013

SAR

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In order to assess the suitable explant(s) for in-vitro regeneration of three local cultivars of Nigerian tomatoes, Ibadan local (IbL), Ife and JM94/46, cotyledon, hypocotyls and radicle explants were cultured in shoot regeneration medium consisting of MS containing 30 g L -1 sucrose and 8 g L -1 agar with no exogenous plant growth hormones. Forty-five of each explant type was cultured on the medium in triplicate experiments and results showed varied percentage survival and shooting for the various explants. Hypocotyl explants had the highest percentage of shooting explants at 13.3% for IbL; 6.67% for Ife and 20% in JM94/46. IbL cotyledon explants had 4.44% of shooting explants with no shoots recorded in Ife and JM94/46 cotyledon explants. IbL radicle explants had 2.22% shooting explants and no shoots recorded in Ife and JM94/46. Student Neuman Keuls (SNK) statistical analysis of cultivar-media interaction showed there was no significant difference (P > 0.05) among the three cultivars in number of calli and shooting calli. There was however significant difference among the cultivars in the number of shoots recorded. SNK values for explants-media interaction showed that cotyledon and radicle explants were significantly different (P < 0.05) from hypocotyl explants in the number of shoots produced.

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Section: Discussionmentioning

confidence: 99%

Effect of Cultivar and Explants Type on Tissue Culture Regeneration of Three Nigerian Cultivars of Tomatoes

Ajenifujah-Solebo¹,

Isu²,

Olorode³

et al. 2013

SAR

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“…One is by the reactivation of relatively undifferentiated cells existing in the somatic tissue and the other is by the reprogramming of mature somatic cells (Birnbaum and Sánchez Alvarado, 2008;Tanaka and Reddien, 2011;Ikeuchi et al, 2016). In some cases, these initiating cells directly regenerate new organs, but in other cases they first develop callus, a mass of dividing cells, from which new organs form (Hicks, 1994).…”

Section: Introductionmentioning

confidence: 99%

WIND1 Promotes Shoot Regeneration through Transcriptional Activation of ENHANCER OF SHOOT REGENERATION1 in Arabidopsis

et al. 2016

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Many plant species display remarkable developmental plasticity and regenerate new organs after injury. Local signals produced by wounding are thought to trigger organ regeneration but molecular mechanisms underlying this control remain largely unknown. We previously identified an AP2/ERF transcription factor WOUND INDUCED DEDIFFERENTIATION1 (WIND1) as a central regulator of wound-induced cellular reprogramming in plants. In this study, we demonstrate that WIND1 promotes callus formation and shoot regeneration by upregulating the expression of the ENHANCER OF SHOOT REGENERATION1 (ESR1) gene, which encodes another AP2/ERF transcription factor in Arabidopsis thaliana. The esr1 mutants are defective in callus formation and shoot regeneration; conversely, its overexpression promotes both of these processes, indicating that ESR1 functions as a critical driver of cellular reprogramming. Our data show that WIND1 directly binds the vascular system-specific and wound-responsive cis-element-like motifs within the ESR1 promoter and activates its expression. The expression of ESR1 is strongly reduced in WIND1-SRDX dominant repressors, and ectopic overexpression of ESR1 bypasses defects in callus formation and shoot regeneration in WIND1-SRDX plants, supporting the notion that ESR1 acts downstream of WIND1. Together, our findings uncover a key molecular pathway that links wound signaling to shoot regeneration in plants.

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“…The gene expression and proteomic profile analyses of Arabidopsis root or cotyledon explants on CIM showed that profound changes occurred in both the transcriptome and proteome during callus induction [6,10,11]. However, since direct organogenesis has been observed when some of plant tissues or organs were cultured on SIM or RIM [12,13], there is another possibility that some kind of pre-existing cells within explants are potentially stem cell-like, and might selectively proliferate to form callus. The developmental events during callus induction were characterized in Arabidopsis only recently.…”

Section: Introductionmentioning

confidence: 99%

LATERAL ORGAN BOUNDARIES DOMAIN transcription factors direct callus formation in Arabidopsis regeneration

et al. 2012

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The remarkable regeneration capability of plant tissues or organs under culture conditions has underlain an extensive practice for decades. The initial step in plant in vitro regeneration often involves the induction of a pluripotent cell mass termed callus, which is driven by the phytohormone auxin and occurs via a root development pathway. However, the key molecules governing callus formation remain unknown. Here we demonstrate that Arabidopsis LATERAL ORGAN BOUNDARIES DOMAIN (LBD)/ASYMMETRIC LEAVES2-LIKE (ASL) transcription factors are involved in the control of callus formation program. The four LBD genes downstream of AUXIN RESPONSE FACTORs (ARFs), LBD16, LBD17, LBD18 and LBD29, are rapidly and dramatically induced by callus-inducing medium (CIM) in multiple organs. Ectopic expression of each of the four LBD genes in Arabidopsis is sufficient to trigger spontaneous callus formation without exogenous phytohormones, whereas suppression of LBD function inhibits the callus formation induced by CIM. Moreover, the callus triggered by LBD resembles that induced by CIM by characteristics of ectopically activated root meristem genes and efficient regeneration capacity. These findings define LBD transcription factors as key regulators in the callus induction process, thereby establishing a molecular link between auxin signaling and the plant regeneration program.

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Shoot induction and organogenesis in vitro: A developmental perspective

Cited by 74 publications

References 78 publications

Effect of Cultivar and Explants Type on Tissue Culture Regeneration of Three Nigerian Cultivars of Tomatoes

Effect of Cultivar and Explants Type on Tissue Culture Regeneration of Three Nigerian Cultivars of Tomatoes

WIND1 Promotes Shoot Regeneration through Transcriptional Activation of ENHANCER OF SHOOT REGENERATION1 in Arabidopsis

LATERAL ORGAN BOUNDARIES DOMAIN transcription factors direct callus formation in Arabidopsis regeneration

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