In order to assess the suitable explant(s) for in-vitro regeneration of three local cultivars of Nigerian tomatoes, Ibadan local (IbL), Ife and JM94/46, cotyledon, hypocotyls and radicle explants were cultured in shoot regeneration medium consisting of MS containing 30 g L -1 sucrose and 8 g L -1 agar with no exogenous plant growth hormones. Forty-five of each explant type was cultured on the medium in triplicate experiments and results showed varied percentage survival and shooting for the various explants. Hypocotyl explants had the highest percentage of shooting explants at 13.3% for IbL; 6.67% for Ife and 20% in JM94/46. IbL cotyledon explants had 4.44% of shooting explants with no shoots recorded in Ife and JM94/46 cotyledon explants. IbL radicle explants had 2.22% shooting explants and no shoots recorded in Ife and JM94/46. Student Neuman Keuls (SNK) statistical analysis of cultivar-media interaction showed there was no significant difference (P > 0.05) among the three cultivars in number of calli and shooting calli. There was however significant difference among the cultivars in the number of shoots recorded. SNK values for explants-media interaction showed that cotyledon and radicle explants were significantly different (P < 0.05) from hypocotyl explants in the number of shoots produced.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.