Shining light in blind alleys: deciphering bacterial attachment in silicon microstructures

Abstract

With new advances in infectious disease, antifouling surfaces, and environmental microbiology research comes the need to understand and control the accumulation and attachment of bacterial cells on a surface. Thus,...

A 3D-printed microfluidic gradient generator with integrated photonic silicon sensors for rapid antimicrobial susceptibility testing

“…67 The PRISM assay enables real-time monitoring of bacterial growth by analyzing changes in the reflectance spectra of the photonic chip over time. 43,68 Fig. 5A-iii shows a characteristic reflectance spectrum at a single time point, which is analyzed to track bacterial growth by monitoring the 2nL value (where n is the refractive index of the medium within the periodic microwells, and L represents the depth of the wells) as detailed in Fig.…”

Disregarded Free Chains Affect Bacterial Adhesion on Cross-Linked Polydimethylsiloxane Surfaces

“…Bacterial biofilms are steadfast gatherings of microbial organisms that instigate tenacious biofouling and biocorrosion in industrial domains and unrelenting infections that afflict humanity . To understand the dynamic process of biofilm formation, polydimethylsiloxane (PDMS) with chemically cross-linked networks (CPDMS), as one of the most popular elastomers, has been often exploited as a model material to study bacteria–material interactions due to its wide utilization in medical fields for catheters, contact lenses, medical adhesives, implants, etc. , Over the past decades, researchers have done extensive work showing that bacterial adhesions on CPDMS are regulated by various physicochemical properties of the surface, such as morphology, − stiffness, − and wettability. , However, most of these studies have overlooked the potential influence of uncross-linked free chains of PDMS that dissociate in CPDMS on bacterial adhesion behavior. Therefore, the majority of current investigations regarding the adherence of bacteria onto CPDMS surfaces have been conducted in bivariate circumstances.…”

Self-assembled fatty acid crystalline coatings display superhydrophobic antimicrobial properties

“…S5 a, b), bacteria may not be fully exposed to the microscope laser beam using CLSM technique due to their adhesion to hidden locations on the surface and could result in their lower detection. Moreover, surface structure is one of the factors that may strongly affect the bacteria adhesion and their behavior on the surfaces [ [67] , [68] , [69] ]. Similar to the CLSM results, bacterial cells density on the SFA coatings is profoundly reduced in comparison to the uncoated substrates ( Fig.…”