Shift in the subgingival microbiome following scaling and root planing in generalized aggressive periodontitis

Liu, Guojing; Luan, Qingxian; Chen, Feng; Chen, Zhibin; Zhang, Qian; Yu, Xiaoqian

doi:10.1111/jcpe.12862

Cited by 43 publications

(95 citation statements)

References 69 publications

(86 reference statements)

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“…This diversity difference remained in the young animals even after ligation, while the profiles in the adolescent animals began to approach 60% of the OTUs by 1 month postligation, similar to the maintenance of 60% to 70% of the OTUs during disease in the older animals. This type of finding has been reported for human periodontitis with lower diversity in disease samples, albeit findings vary (62)(63)(64)(65). It has also been reported that while members of the oral microbiome may differ across individuals, the functional genomics of the bacterial population are more similar in health, with substantial changes in the gene expression profiles of commensals with disease (9,20,66).…”

Section: Discussionsupporting

confidence: 60%

“…Finally, it is clear that standard nonsurgical therapy of scaling and root planing lowers the microbial burden in periodontitis and decreases inflammation as an approach to controlling the disease (44,62,64). However, while the majority of the population responds well to this treatment, a portion of the population continues to demonstrate disease, with little predictive understanding of who constitutes this "nonresponding" group (70)(71)(72)(73).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Microbiome Profiles of Ligature-Induced Periodontitis in Nonhuman Primates across the Life Span

Kirakodu

Chen

Martinez³

et al. 2019

Infect Immun

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This investigation compared the microbiomes colonizing teeth during the initiation, progression, and resolution of periodontitis in nonhuman primates (Macaca mulatta) at different ages. Subgingival plaque samples were collected at baseline; 0.5, 1, and 3 months following ligature-induced periodontitis; and following naturally occurring disease resolution at 5 months. Samples were analyzed using 16S amplicon sequencing to identify bacterial profiles across age groups: young (<3 years of age), adolescent (3 to 7 years), adult (12 to 15 years), and aged (17 to 23 years). α-Diversity of the microbiomes was greater in the adult/aged samples than in the young/adolescent samples. β-Diversity of the samples demonstrated clear age group differences, albeit individual variation in microbiomes between animals within the age categories was noted. Phylum distributions differed between the young/adolescent animals and the adult/aged animals at each of the time points, showing an enrichment of the phyla Spirochetes, Fusobacteria, and Bacteroidetes associated with periodontitis. Major differences in the top 50 operational taxonomic units (OTUs) were noted in the young and adolescent microbiomes during initiation and progression postligation compared to the adult and aged animals. The proportions of a large number of species in the top 50 OTUs were lower at baseline and in resolved disease microbiomes in the young samples, while profiles in adolescent animals were more consistent with the disease microbiomes. Microbiome profiles for resolution for adults and aged animals appeared more resilient and generally maintained a pattern similar to that of disease. Use of the model can expand our understanding of the crucial interactions of the oral microbiome and host responses in periodontitis.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Microbiome Profiles of Ligature-Induced Periodontitis in Nonhuman Primates across the Life Span

Kirakodu

Chen

Martinez³

et al. 2019

Infect Immun

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“…Calculation of the Shannon and Simpson diversity indices did not display significant differences over time in the present study, indicating an overall resilience of the salivary microbiome to external pressures, one such being periodontal treatment. Earlier diversity analyses of the subgingival [ 24 ] or salivary [ 28 ] microbiome of AgP did not reveal significant differences post‐treatment either, in line with the present findings. On the contrary, treatment of chronic periodontitis is shown to cause significant reduction in the diversity of the salivary microbiome, [ 28 ] but not necessarily significant differences in the abundance of the predominant bacterial species.…”

Section: Discussionsupporting

confidence: 91%

“…[ 21 ] Microbial shifts following treatment have also been studied, but with less consistent findings. [ 8,22–24 ] Divergences in the microbial profiles according to the treatment outcome (successful or not) are also not well characterized. [ 25 ]…”

Section: Introductionmentioning

confidence: 99%

Salivary Microbiome Shifts in Response to Periodontal Treatment Outcome

Greenwood

Afacan

Emingil

et al. 2020

Proteomics Clinical Apps

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Purpose Periodontitis is linked to a localized dysbiotic microbial shift. This trending may often not be evident due to deep taxonomic changes of low abundance organisms and lack of consideration of variations in the treatment response. By using next generation sequencing this study aims to evaluate the salivary microbiome dynamics of periodontal treatment and the implication of treatment outcome Experimental Design Patients with generalized aggressive periodontitis are treated non‐surgically and followed up for 6 months. Saliva is collected for microbiome profiling by next generation sequencing and diversity analysis, as well as quantitative real‐time polymerase chain reaction (qPCR). The treatment outcome on the first follow‐up is also considered. Results Clinical parameters are significantly improved following treatment, but with no accompanying relative abundance changes on the phylum, genus and species levels, or diversity indices. Distinctive differences are observed on species level when the sensitive qPCR is used. Patients responding poorly to treatment display a marginally lower microbiome profile distance from baseline, compared to those responding favorably. Conclusion and Clinical Relevance Periodontal treatment does not alter the broader salivary microbiome profile, but may have selective implications on the species level. Treatment outcome can be impactful in the microbiome profile, as reduced microbiome changes may be associated with poorer clinical responses.

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“…The characteristics of the subgingival microbiome in patients with periodontitis and its shift after clinical intervention have been investigated. It was demonstrated that patients with periodontitis harboured a more pathogenic microbial community than healthy individuals (Abusleme et al, ), and the proportion of periodontitis‐associated taxa decreased and the proportion of health‐associated taxa increased after periodontal treatment (Liu et al, ; Shi et al, ). Then, the bacteria recolonized in periodontal pockets.…”

Section: Introductionmentioning

confidence: 99%

Microbiome in maintained periodontitis and its shift over a single maintenance interval of 3 months

Zhao

Feng

et al. 2019

J Clinic Periodontology

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Aim To assess the subgingival microbial shift of maintained periodontitis treated by ultrasonic scaling (US) or air polishing (AP) during a 3‐month maintenance interval. Materials and methods We conducted a 12‐week randomized split‐mouth controlled trial with US and AP in 17 maintained subjects (bleeding on probing [BOP%] ≤25%, probing depth [PD] ≤5 mm). They were monitored at baseline, week 2, week 8 and week 12. The V3‐V4 region of the 16S rDNA from 136 subgingival plaque samples was sequenced and analysed. Results Treatment by US or AP could effectively reduce the PD, microbial richness, diversity, periodontitis‐associated microbiota and pathogenic metabolism in maintained periodontitis. Bacteria recolonized after treatment and returned to the pre‐treatment level 12 weeks after treatment. Ultrasonic scaling group demonstrated slight advantage in reducing BOP (%), pathogenic bacteria and metabolism than AP group. Pathogenic microbiota and commensal microbiota kept a balance in subgingival community of maintained patients during the 3‐month interval. Conclusions Treatment by US or AP effectively reduced the pathogenicity of subgingival microbiome by reducing microbial diversity, proportion of periodontitis‐associated microbiota and pathogenic metabolism. It helped to keep a balanced subgingival community and stable periodontal condition over a single maintenance interval of 3 months.

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Shift in the subgingival microbiome following scaling and root planing in generalized aggressive periodontitis

Cited by 43 publications

References 69 publications

Microbiome Profiles of Ligature-Induced Periodontitis in Nonhuman Primates across the Life Span

Microbiome Profiles of Ligature-Induced Periodontitis in Nonhuman Primates across the Life Span

Salivary Microbiome Shifts in Response to Periodontal Treatment Outcome

Microbiome in maintained periodontitis and its shift over a single maintenance interval of 3 months

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