Shewanella gelidii sp. nov., isolated from the red algae Gelidium amansii, and emended description of Shewanella waksmanii

Wang, Yan; Chen, Hongli; Liu, Zhenhua; Hong, Ming; Zhou, Chenyan; Zhu, Xiao-liang; Zhang, Peng; Jing, Changqin; Hong, Feng

doi:10.1099/ijsem.0.001075

“…Enzymatic Table 1. Phenotypic traits and other features that differentiate strain SM1901 T from the type strains of closely related species Strains: 1, SM1901 T 2, Shewanella litoralis JCM 32306 T (data from this study); 3, Shewanella livingstonensis MCCC 1C00016 T (data from this study); 4, Shewanella vesiculosa MCCC 1A0002 T (data from this study); 5, Shewanella saliphila MMS16-UL250 T (data from [8]); 6, Shewanella ulleungensis MMS16-UL253 T (data from [8]); 7, Shewanella putrefaciens ATCC 8071 T (data from [43][44][45][46]). +, Positive; -, negative; nd, no data available.…”

Section: Chemotaxonomic and Phenotypic Characterizationmentioning

confidence: 84%

Shewanella polaris sp. nov., a psychrotolerant bacterium isolated from Arctic brown algae

Cha

¹

,

Ren

²

,

Sun

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, facultatively anaerobic, flagellated and rod-shaped bacterium, designated strain SM1901T, was isolated from a brown algal sample collected from Kings Bay, Svalbard, Arctic. Strain SM1901T grew at −4‒30 °C and with 0–7.0 % (w/v) NaCl. It reduced nitrate to nitrite and hydrolysed DNA and Tween 80. Results of phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SM1901T was affiliated with the genus Shewanella , showing the highest sequence similarity to the type strain of Shewanella litoralis (97.5%), followed by those of Shewanella vesiculosa , Shewanella livingstonensis and Shewanella saliphila (97.3 % for all three). The major cellular fatty acids were summed feature 3 (C16 : 1 ω7с and/or C16 : 1 ω6с), C16 : 0, C18 : 0, iso-C15 : 0 and C17 : 1 ω8с and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The respiratory quinones were ubiquinones Q-7, Q-8, menaquinones MK-7(H) and MK-8. The genome of strain SM1901T was 4648537 nucleotides long and encoded a variety of cold adaptation related genes, providing clues for better understanding the ecological adaptation mechanisms of polar bacteria. The genomic DNA G+C content of strain SM1901T was 40.5 mol%. Based on the polyphasic evidence presented in this paper, strain SM1901T was considered to represent a novel species, constituting a novel psychrotolerant lineage out of the known SF clade encompassed by polar Shewanella species, within the genus Shewanella , for which the name Shewanella polaris sp. nov. is proposed. The type strain is SM1901T (=KCTC 72047T=MCCC 1K03585T).

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“…Most of the clinically relevant isolates seem to belong to the species S. alga (Nozue et al., ). Meanwhile, many additional species of the genus Shewanella were described, such as S. abyssi (Miyazaki, Nogi, Usami, & Horikoshi, ), S. affinis (Ivanova et al., ), S. algicola (Kim et al., ), S. algidipiscicola (Satomi, Vogel, Venkateswaran, & Gram, ) , S. amazonensis (Venkateswaran, Dollhopf, Aller, Stackebrandt, & Nealson, ), S. aquimarina (Yoon, Yeo, Kim, & Oh, ), S. arctica (Kim et al., ), S. atlantica (Zhao, Manno, Thiboutot, Ampleman, & Hawari, ), S. basaltis (Chang et al., ), S. benthica (Lauro et al., ), S. canadensis (Zhao et al., ), S. chilikensis (Sucharita et al., ), S. colwelliana (Labare & Weiner, ) , S. corallii (Shnit‐Orland, Sivan, & Kushmaro, ) , S. decolorationis (Xu et al., ) , S. denitrificans (Brettar, Christen, & Hofle, ) , S. dokdonensis (Sung, Yoon, & Ghim, ) , S. donghaensis (Yang, Lee, Ryu, Kato, & Kim, ), S. fidelis (Ivanova et al., ) , S. fodinae (Kumar et al., ) , S. frigidimarina (Bowman et al., ; Bozal, Montes, Tudela, Jimenez, & Guinea, ) , S. gaetbuli (Yoon, Kang, Oh, & Park, ) , S. gelidimarina (Bowman et al., ), S. gelidii (Wang et al., ) , S. glacialipiscicola (Satomi et al., ) , S. hafniensis (Satomi, Vogel, Gram, & Venkateswaran, ) , S. halifaxensis (Zhao, Manno, Leggiadro, O'Neill, & Hawari, ) , S. halitois (Kim et al., ) , S. hanedai (Jensen, Tebo, Baumann, Mandel, & Nealson, ) , S. indica (Verma et al., ) , S. inventionis (Wang & Sun, ) , S. irciniae (Lee et al., ) , S. japonica (Ivanova et al., ) , S. kaireitica (Miyazaki et al., ) , S. litorisediminis (Lee & Yoon, ) , S. livingstonensis (Bozal et al., ) , S. loihica (Gao et al., ) , S. mangrovi (Liu, Shang, Yi, Gu, & Zeng, ) , S. marina (Park, Baik, Kim, Kim, & Seong, ), S. marinintestina (Satomi, Oikawa, & Yano, ), S. marisflavi (Yoon, Yeo, et al., ), S. morhuae (Satomi et al., ), S. olleyana (Skerratt, Bowman, & Nichols, …”

Section: Introductionmentioning

confidence: 99%

Methods for identification and differentiation of different Shewanella spp. isolates for diagnostic use

Jung‐Schroers

¹

,

Jung

²

,

Ryll

³

et al. 2017

Journal of Fish Diseases

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Shewanella spp. are Gram-negative, rod-shaped, motile bacteria that are widely distributed in marine and freshwater environments. The bacteria are present in the physiological microflora of fish from temperate waters and are known as fish spoilage species. From clinically healthy fish and from fish with skin ulcerations, Shewanella spp. is regularly isolated, indicating a possible role as fish pathogen. In this study, 74 isolates of Shewanella spp. were analysed. For species identification, biochemical techniques, 16S rRNA sequencing, MALDI-TOF MS and the Sherlock Microbial Identification System (MIS) based on the composition of fatty acid ethyl esters were compared. The phylogenetic relationship, cytotoxicity in vitro and resistance against antibiotics were tested. The most reliable method for species identification was 16S rRNA sequencing. From diseased fish, clinically healthy fish and the aquatic environment, different Shewanella species were isolated. This indicates that Shewanella spp. is widespread in the aquatic milieu and acts as a secondary pathogen. The virulence of Shewanella spp. is probably not depending on the species but on the isolate itself. Many isolates of Shewanella spp. were showing multiresistances against antibiotic substances, especially in samples derived from retailers and in routine diagnostics, all Shewanella spp. should therefore be tested for resistances against antibiotic agents.

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“…The strain could hydrolyze DNA, gelatin, starch and, casein. The phenotypic characteristics that differentiate the strain from putrefaciens JCM 20190 T , S. baltica DSM 9439 T , S. profunda LT13a T and S. hafniensis P010 T are given in Table 2, [36,[39][40][41][42][43][44]. The complete list of phenotypic characteristics are shown in Table S1 (available in the online version of this article).…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…profunda LT13a T and S. hafniensis P010 T are given in , [36, 39–44]. The complete list of phenotypic characteristics are shown in Table S1 (available in the online version of this article).…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Shewanella oncorhynchi sp. nov., a novel member of the genus Shewanella, isolated from Rainbow Trout (Oncorhynchus mykiss)

Altun

¹

,

Duman

²

,

Ay

³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A strain, S-1T was isolated from rainbow trout (Oncorhynchus mykiss) exhibiting clinical symptoms of lens atrophy, inappetence, visual impairment and growth retardation. The strain was identified as representing a member of the genus Shewanella on the basis of the results of 16S rRNA gene sequence analysis. The neighbor-joining phylogenetic tree based on 16S rRNA gene sequences indicated that S-1T clustered with Shewanella putrefaciens JCM 20190T, Shewanella profunda DSM 15900T, and Shewanella hafniensis P010T, sharing 99.3, 98.8 and 87.7% 16S rRNA gene similarities, respectively. A polyphasic taxonomic approach including phenotypic, chemotaxonomic, and genomic characterization was employed to ascertain the taxonomic position of S-1T within the genus Shewanella . The overall genome relatedness indices (OGRI) for S-1T compared with the most closely related type strains S. hafniensis ATCC BAA-1207T, Shewanella baltica NCTC 10735T, S. putrefaciens ATCC 8071T and S. profunda DSM 15900T were calculated as 40.8, 40.1, 28.5 and 27.3% for digital DNA–DNA hybridization (dDDH), and 91.6, 91.0, 86.3 and 85.1% for average nucleotide identity (ANI), respectively. OGRI values between S-1T and its close neighbours confirmed that the strain represents a novel species in the genus Shewanella .The DNA G+C content of the strain is 45.2%. Major fatty acids were C17 : 1ω8c, C15 : 0iso, and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The predominant polar lipids were phosphatidylethanolamine, phospholipid, amino-phospholipid and unidentified lipids. The major respiratory quinones were ubiquinone-8, ubiquinone-7 and menaquinone-7. Chemotaxonomic and phylogenomic analyses of this isolate confirmed that the strain represents a novel species for which the name Shewanella oncorhynchi sp. nov. is proposed, with S-1T as the type strain (JCM 34183T= KCTC 82249T).

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Shewanella gelidii sp. nov., isolated from the red algae Gelidium amansii, and emended description of Shewanella waksmanii

Cited by 14 publications

References 22 publications

Shewanella polaris sp. nov., a psychrotolerant bacterium isolated from Arctic brown algae

Shewanella polaris sp. nov., a psychrotolerant bacterium isolated from Arctic brown algae

Methods for identification and differentiation of different Shewanella spp. isolates for diagnostic use

Shewanella oncorhynchi sp. nov., a novel member of the genus Shewanella, isolated from Rainbow Trout (Oncorhynchus mykiss)

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