Shewanella aestuarii sp. nov., a marine bacterium isolated from a tidal flat

Abstract

A Gram-stain-negative, non-spore-forming, facultatively anaerobic bacterium, designated strain SC18 T , was isolated from a tidal flat of Suncheon bay in South Korea. Cells were rod-shaped and motile by means of a polar flagellum. Cells were catalase-, oxidase-and b-haemolysis-positive.Growth was observed at 4-37 6C (optimum, 25-30 6C), at pH 5.0-9.0 (optimum, pH 7.0) and in the presence of 0-5.0 % (w/v) NaCl (optimum, 0-2 %). The major cellular fatty acids were summed feature 3 (comprising C 16 : 1 v7c and/or C 16 : 1 v6c), iso-C 15 : 0 and C 16 : 0 . The polar lipid pattern indicated the presence of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmethylethanolamine, an unidentified aminolipid and three unidentified lipids. Strain SC18 T contained Q-7, Q-8, MK-7 and MMK-7 as the dominant respiratory quinones and the G+C content of the genomic DNA was 41.3 mol%. Phylogenetic analysis based on 16S rRNA and gyrase B (gyrB) gene sequences showed that strain SC18 T formed a tight phyletic lineage with members of the genus Shewanella. Strain SC18 T was related most closely to Shewanella denitrificans OS217 T (97.3 % 16S rRNA gene sequence similarity) and Shewanella gaetbuli TF-27 T (97.1 %), but the DNA-DNA relatedness levels between strain SC18 T and the type strains of S. denitrificans and S. gaetbuli were 18.3±2.8 and 22.5±1.6 % (mean±SD), respectively. On the basis of phenotypic, chemotaxonomic and molecular features, strain SC18

Shewanella algicola sp. nov., a marine bacterium isolated from brown algae

“…S3). The presence of phosphatidylethanolamine and phophatidylglycerol was consistent with analyses of other species of the genus Shewanella (Toffin et al, 2004;Kim et al, 2012;Park & Jeon, 2013).…”

“…The isoquinones were in accordance with those of other members of the genus Shewanella (Venkateswaran et al, 1999;Yoon et al, 2004Yoon et al, , 2012Gao et al, 2006;Lee et al, 2006;Sung et al, 2012;. The DNA G+C content of strain T was 42.4 mol %, which fell within the range of 41-58 mol% reported for other species of the genus Shewanella Park & Jeon, 2013). The polar lipid profile of strain ST-6 T consisted of phosphatidylethanolamine, phophatidylglycerol, unknown aminolipid and six unknown lipids.…”

“…Members of the genus Shewanella are widely distributed in marine environments including seawater (Makemson et al, 1997;Brettar et al, 2002;Park et al, 2009;Sung et al, 2012), sediment (Venkateswaran et al, 1999;Toffin et al, 2004;Gao et al, 2006;Chang et al, 2008;Pankaj et al, 2011;Kim et al, 2012;Yoon et al, 2012), tidal flats (Yoon et al, 2004Park & Jeon, 2013), marine invertebrates (Ivanova et al, 2004b;Lee et al, 2006;Yang et al, 2006) and fish (Satomi et al, 2006(Satomi et al, , 2007, and Antarctic sea ice (Bowman et al, 1997;Bozal et al, 2002Bozal et al, , 2009, and some species are found in activated sludge (Xu et al, 2005) and clinical samples (Brink et al, 1995;Debois et al, 1975;Holmes et al, 1975;Nozue et al, 1992).…”

Multilocus Sequence Analysis, a Rapid and Accurate Tool for Taxonomic Classification, Evolutionary Relationship Determination, and Population Biology Studies of the Genus Shewanella

“…The disadvantage of the application of the 16S rRNA gene was the low resolving power to discriminate closely related species due to their high sequence similarities (19). Recently, a more rapidly evolving housekeeping gene (HKG) of gyrB was selected as an alternative phylogenetic indicator for Shewanella species classification (7,(20)(21)(22). Nevertheless, the quality of sequences submitted in public databases is poor (22)(23)(24).…”

Shewanella gelidii sp. nov., isolated from the red algae Gelidium amansii, and emended description of Shewanella waksmanii

“…Thus, the fatty acid profile supports that RZB5-4 T represents a novel taxon. In accordance with other members of the genus Shewanella (Bozal et al, 2002;Brettar et al, 2002;Park & Jeon, 2013), strain RZB5-4 T contained simultaneously menaquinones and ubiquinones. The respiratory quinones detected in strain RZB5-4 T were menaquinone 7 (MK7), ubiquinone 7 (Q7), ubiquinone 8 (Q8) and methylmenaquinone 7 (MMK7).…”