Shen-Ling-Bai-Zhu-San alleviates functional dyspepsia in rats and modulates the composition of the gut microbiota

Zhang, Shaobao; Lei, Lin; Li, Wen; Zou, Baorong; Cai, Ying; Liu, Deliang; Xiao, Dan; Chen, Jiahui; Li, Pei; Zhong, Yuanyuan; Liao, Qiongfeng; Xie, Zenghong

doi:10.1016/j.nutres.2019.10.001

Cited by 28 publications

(22 citation statements)

References 55 publications

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“…These changes were unexpected because we initially believed that the FD modeling measures employed would produce permanent pathophysiological changes such as permanent increases in weight loss as noted by Wu et al [ 23 ]. However, similar trends were shown in another study by Zhang et al measuring the effects of Shen-Ling-Bai-Zhu-San on FD rat models [ 33 ], where the FD model group exhibited increases in food and water intake without intervention. Here, the data seemed to show other factors at work which decreased the effects of FD modeling on the FD model group, despite this group not being subject to EA intervention.…”

Section: Resultssupporting

confidence: 83%

The Influence of Stomach Back-Shu and Front-Mu Points on Insular Functional Connectivity in Functional Dyspepsia Rat Models

Chen

Zhao

Tan

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Functional Dyspepsia (FD) is a common functional gastrointestinal disease, which can reduce the quality of life in patients. Prior research has indicated that insula is closely related to FD and that acupuncture can regulate the functional connectivity (FC) of FD. Therefore, we hypothesized that acupuncture on FD was effected through the insular pathway. To test our hypothesis, we performed electroacupuncture (EA) on FD rat models and then examined the FC between insula and other brain regions through resting-state functional magnetic resonance imaging (rs-fMRI). Seven-day-old male infant Sprague-Dawley (SD) rats were randomly divided into control group, FD model group, and FD acupuncture group, with twelve rats per group (n = 36). Upon establishing successful models, the FD acupuncture group was subjected to EA intervention using Stomach back-shu (BL-21) and front-mu (RN-12) points for ten consecutive days for durations of 20 minutes each day. After intervention, each group was subject to rs-fMRI. The digital image data obtained were analyzed using FC analysis methods. Subsequently, gastric ligation was performed to measure gastric emptying rates. Before EA intervention, the FD model group exhibited decreased functional connections between the insula and a number of brain regions. After EA intervention, FD acupuncture group exhibited increasing FC between insula and regions when compared to the FD model group, such as the primary somatosensory cortex (S1), hippocampal CA3 (CA3), polymorphic layer of dentate gyrus (PoDG), caudate putamen (CPu), and oral pontine reticular nuclei (PnO) P < 0.05 ; decreasing FC was also exhibited between insula and regions such as the bilateral primary and secondary motor cortexes (M1/2), paraventricular hypothalamic nucleus (PVA), and limbic cortex (LC). These findings indicate that the effective treatment of FD using EA may be through regulating the abnormal FC between insula and several brain regions, in particular CA3, PoDG, and PVA.

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Section: Resultssupporting

confidence: 83%

The Influence of Stomach Back-Shu and Front-Mu Points on Insular Functional Connectivity in Functional Dyspepsia Rat Models

Chen

Zhao

Tan

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…On the other hand, the abundances of Mucispirillum, Bilophila, Ruminiclostridium increased after TEDP adminstration. Zhang, et al 36 found that Shen‐Ling‐ Bai‐Zhu‐San could improve functional dyspepsia by reducing functional dyspepsia biomarkers including Prevotella, Mucispirillum, and Akkermansia. Bilophila wadsworthia, which belong to the Bilophila genera, had been proved to promote higher inflammation, intestinal barrier dysfunction and bile acid dysmetabolism, leading to higher glucose dysmetabolism and hepatic steatosis, 37 and it had also been confirmed to be associated with colorectal cancer 38 .…”

Section: Discussionmentioning

confidence: 99%

Gut microbiota disorder caused by diterpenoids extracted from Euphorbia pekinensis aggravates intestinal mucosal damage

Wang

Maimaiti

et al. 2021

Pharmacology Res & Perspec

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Gut microbiota disorder will lead to intestinal damage. This study evaluated the influence of total diterpenoids extracted from Euphorbia pekinensis (TDEP) on gut microbiota and intestinal mucosal barrier after long‐term administration, and the correlations between gut microbiota and intestinal mucosal barrier were analysed by Spearman correlation analysis. Mice were randomly divided to control group, TDEP groups (4, 8, 16 mg/kg), TDEP (16 mg/kg) + antibiotic group. Two weeks after intragastric administration, inflammatory factors (TNF‐α, IL‐6, IL‐1β) and LPS in serum, short chain fatty acids (SCFAs) in feces were tested by Enzyme‐linked immunosorbent assay (ELISA) and high‐performance liquid chromatography (HPLC), respectively. The expression of tight junction (TJ) protein in colon was measured by western blotting. Furthermore, the effects of TDEP on gut microbiota community in mice have been investigated by 16SrDNA high‐throughput sequencing. The results showed TDEP significantly increased the levels of inflammatory factors in dose‐dependent manners, and decreased the expression of TJ protein and SCFAs, and the composition of gut microbiota of mice in TDEP group was significantly different from that of control group. When antibiotics were added, the diversity of gut microbiota was significantly reduced, and the colon injury was more serious. Finally, through correlation analysis, we have found nine key bacteria (Barnesiella, Muribaculaceae_unclassified, Alloprevotella, Candidatus_Arthromitus, Enterorhabdus, Alistipes, Bilophila, Mucispirillum, Ruminiclostridium) that may be related to colon injury caused by TDEP. Taken together, the disturbance of gut microbiota caused by TDEP may aggravate the colon injury, and its possible mechanism may be related to the decrease of SCFAs in feces, disrupted the expression of TJ protein in colon and increasing the contents of inflammatory factors.

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“…Moreover, Intestinimonas could increase in abundance in animal models of inflammation [ 14 ]. Mucispirillum showed high abundance in the intestinal tract with oxidative stress and inflammation, which was also a sign of serious indigestion [ 32 , 33 ]. After thoracic irradiation, Helicobacter , Alisipes , Parabacteroides , Bacteroides , Intestinimonas, and Mucispirillum in the intestine proliferated in large quantities.…”

Section: Discussionmentioning

confidence: 99%

Effects of phycocyanin on pulmonary and gut microbiota in a radiation-induced pulmonary fibrosis model

Li¹,

Lu²,

Liu³

et al. 2020

Biomedicine & Pharmacotherapy

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Objective Radiation pneumonia and fibrosis are major clinical complications of radiotherapy for thoracic tumor patients, and may significantly reduce survival and quality of life. At present, no safe and effective radiation protection measures have been approved for clinical use. Phycocyanin, a protein responsible for photosynthesis from Spirulina , has been shown to have a variety of biological activities and to be beneficial for a variety of diseases, including pulmonary fibrosis. However, the preventive and protective effects of phycocyanin on radiation-induced pulmonary fibrosis have not been studied. Design X-ray single dose irradiation was used on the chest of mice to prepare a mouse model of pulmonary fibrosis, from which the effect of phycocyanin on pulmonary histopathologic change, pulmonary fibrosis, the microbiota in lung and gut, LPS, TNF-α, and IL-6 at different time after irradiation were evaluated. Results Phycocyanin alleviated the radiation-induced lung injury and reduced the level of inflammatory factors. Thorax irradiation led to the disorder in microbiota of the lung and gut. The variation trend of the diversity of the two tissues was opposite, but that of the microbiota composition was similar. The phycocyanin intervention regulated the composition of the lung and gut microbiota, transformed them into normal state, and reduced the level of LPS, which significantly reduced the abundance of inflammation-related bacteria, and increased the abundance of probiotics that produce short-chain fatty acids. Conclusion Phycocyanin could regulate the radiation-induced disorder in lung and gut microbiota of mice, and reduce the radiation-induced lung inflammation and fibrosis.

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Shen-Ling-Bai-Zhu-San alleviates functional dyspepsia in rats and modulates the composition of the gut microbiota

Cited by 28 publications

References 55 publications

The Influence of Stomach Back-Shu and Front-Mu Points on Insular Functional Connectivity in Functional Dyspepsia Rat Models

The Influence of Stomach Back-Shu and Front-Mu Points on Insular Functional Connectivity in Functional Dyspepsia Rat Models

Gut microbiota disorder caused by diterpenoids extracted from Euphorbia pekinensis aggravates intestinal mucosal damage

Effects of phycocyanin on pulmonary and gut microbiota in a radiation-induced pulmonary fibrosis model

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