Sex-reversal and Histopathological Assessment of Potential Endocrine-Disrupting Effects of Graphene Oxide on Japanese medaka (Oryzias latipes) Larvae

Myla, Anitha; Dasmahapatra, Asok K.; Tchounwou, Paul B.

doi:10.1016/j.chemosphere.2021.130768

Cited by 8 publications

(31 citation statements)

References 74 publications

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“…16 Our laboratory has been evaluating GO, as a potential EDC using Japanese medaka fish (Oryzias latipes), as our experimental model. [17][18][19][20][21][22][23] We have demonstrated that the males of medaka fish are more sensitive to GO than the females. From our histopathological evaluation of the gonadal structures and thyroid follicles, we found that the damages observed after GO administration was mainly due to aggregation/agglomeration of GO on or within the organs rather than directly acting as an EDC.…”

mentioning

confidence: 84%

“…The solution of GO (both 20 and 2000 mg/L) was sonicated for 5 min (2 s on -1 s off pulse, 225 w) using a probe sonicator (ultrasonicator LPX 750, Cole Palmer, Chicago, IL) in ice temperature and further diluted to desired concentration when necessary (for larvae). The sonicated nanomaterial was further checked by transmission electronmicroscope at the Electron Microscopy Core laboratory at the Jackson State University, Jackson, MS. [18][19][20][21]…”

Section: Go Preparationmentioning

confidence: 99%

“…Previously we exposed 1 dph medaka fries (Iwamatsu Stage 40,1st fry stage) to different concentrations (2.5, 5, 10, 20 mg/L in ERM) of GO (Sigma-Aldrich, St. Louis, MO) for 96 h continuously by IMR and assessed the EDC activities of GO on gonads (testis and ovary) and thyroids after 6 weeks post-treatment. [20][21][22][23] In this study, we have evaluated the IRG of some of these larvae by histological/ histochemical methods. Due to technical problems, we were unable to track the IRG of all the larvae we used during our previous experiments.…”

Section: Exposure Of Larvaementioning

confidence: 99%

“…The phenotype and genotype of the larvae was determined by the methods described previously. 20,21 Due to sex-reversal observed in some of the phenotypes (male phenotype with XX genotype), the larvae were then categorized in to three groups; male (testis) phenotypes with XY genotype, male (testis) phenotypes with XX genotype (sex reversed), and female (ovary) phenotypes with XX genotype. We did not observe any female (ovary) phenotype with XY genotype.…”

Section: Phenotype and Genotype Determination Of The Adult And Larvaementioning

confidence: 99%

“…18,19,22,23 Our other investigation of GO effect on the sex reversal (SR) of medaka larvae showed no apparent EDC effect. 20,21 In the present study, we have extended our investigations on the histopathology of the IRG of medaka adults and larvae exposed to different concentrations/doses of GO either by a single intraperitoneal (IP) injection or by 96 h continuous immersion (IMR). In fish, the IRG is considered as the adrenomedullary homolog of the mammalian adrenal gland.…”

mentioning

confidence: 95%

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Histopathological evaluation of the interrenal gland (adrenal homolog) of Japanese medaka (Oryzias latipes) exposed to graphene oxide

Dasmahapatra

2022

Environmental Toxicology

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Due to unique physicochemical properties and wide industrial and biomedical applications, graphene oxide (GO) is ubiquitous in the aquatic ecosystem. Using Japanese medaka (Oryzias latipes) fish as a model, we previously demonstrated minimal endocrine disrupting (ED) effects of GO on reproductive organs, and thyroids. Current study investigated the ED-effects of GO on the interrenal gland (IRG) of medaka.Breeding pairs of adult male and female fish were exposed to 0 mg/L (control) or 20 mg/L GO by continuous immersion for 96 h, or to 0 or 100 μg/g GO by intraperitoneal administration. Also, 1 day post-hatch (dph) larvae were exposed to different concentrations of GO (2.5-20 mg/L) for 96 h. IRG was evaluated by immunohistochemical techniques after 21 days depuration in adults and 6 weeks in larvae. IRG cells were counted and the nuclear area was measured in hematoxylin-eosin stained sections using ImageJ software. We found that IRG is distributed adjacent to the posterior cardinal vein and its branches within the head kidney. Columnar/oval shaped periodic acid-Schiff negative, tyrosine hydroxylase positive cells are arranged either in a single, or in groups, sometimes encircling a sinusoid, or in a straight chord, laying adjacent to the endothelium of the cardinal vein, and having eosinophilic cytoplasm with round/oval basophilic nuclei. GO effect on nuclei and cell population in IRG was inconsistent; depending on exposure route, sex, and/or age of the fish. Also, because of its high adsorptive property and sharp edges, GO probably agglomerated on IRG, and induced physical injury, and ED effects.

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