Single-cell-resolution transcriptome map revealed novel genes involved in testicular germ cell progression and somatic cells specification in Chinese tongue sole with sex reversal

Wang, Hongyan; Liu, Xiang; Chen, Jian-Yang; Huang, Yingyi; Lu, Yao; Tan, Fujian; Li, Qun; Yang, M.; Li, Shuo; Zhang, Xianghui; Qin, Yating; Ma, Wenxiu; Yang, Yingming; Meng, Liang; Liu, Kaiqiang; Wang, Qian; Fan, Guangyi; Nóbrega, Rafael Henrique; Liu, Shanshan; Piferrer, Francesc; Shao, Changwei

doi:10.1007/s11427-021-2236-4

Cited by 12 publications

(9 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We first processed the obtained data using the same analysis method and named cells separately, according to the annotations of their corresponding references [ 41 , 42 ]. Cell-type expression matrices were then integrated using the 1-1-1 orthologous genes found in the three species.…”

Section: Resultsmentioning

confidence: 99%

Single-Cell RNA Sequencing of the Testis of Ciona intestinalis Reveals the Dynamic Transcriptional Profile of Spermatogenesis in Protochordates

Liu

Zhang

et al. 2022

Cells

Self Cite

View full text Add to dashboard Cite

Spermatogenesis is a complex and continuous process of germ-cell differentiation. This complex process is regulated by many factors, of which gene regulation in spermatogenic cells plays a decisive role. Spermatogenesis has been widely studied in vertebrates, but little is known about spermatogenesis in protochordates. Here, for the first time, we performed single-cell RNA sequencing (scRNA-seq) on 6832 germ cells from the testis of adult Ciona intestinalis. We identified six germ cell populations and revealed dynamic gene expression as well as transcriptional regulation during spermatogenesis. In particular, we identified four spermatocyte subtypes and key genes involved in meiosis in C. intestinalis. There were remarkable similarities and differences in gene expression during spermatogenesis between C. intestinalis and two other vertebrates (Chinese tongue sole and human). We identified many spermatogenic-cell-specific genes with functions that need to be verified. These findings will help to further improve research on spermatogenesis in chordates.

show abstract

Section: Resultsmentioning

confidence: 99%

Single-Cell RNA Sequencing of the Testis of Ciona intestinalis Reveals the Dynamic Transcriptional Profile of Spermatogenesis in Protochordates

Liu

Zhang

et al. 2022

Cells

Self Cite

View full text Add to dashboard Cite

show abstract

“…In order to further understand the role of psmd9 in the spermatogenesis of C. semilaevis , overexpression and gene knockdown (RNAi) experiments were carried out on the testicular cell line of C. semilaevis , and the markers of germ cells at various development stages were detected. The types of germ cells were classified as follows: spermatogonia ( dnd1 , stmn1 and zbtb16 ), spermatocytes ( ccne2 , ccnb1 and e2f2 ) and spermatid/spermatozoa ( cfap69 , dnah3 and dnajb13 ) [ 15 ]. In the experiment on the overexpression of psmd9 , the expression level of spermatid/spermatozoa marker genes dnah3 and dnajb13 increased significantly.…”

Section: Discussionmentioning

confidence: 99%

“…Through the analysis of the gonad transcriptome and proteome of adult pseudomale fish and male fish, it was shown that the extracellular environment, such as growth factors and steroid hormones, are not the cause of sperm loss, and there may be many gene regulation defects during sperm maturation, which may lead to sperm maturation being blocked [ 14 ]. On the other hand, high-resolution single-cell RNA-seq maps from the testes of genotypic male and pseudomale fish revealed the dynamic changes in gene expression patterns during spermatogenesis in C. semilaevis [ 15 ]. In particular, the psmd9 gene was significantly differentially expressed between pseudomales and males [ 15 ], and it was located on the Z chromosome [ 9 ], which indicates that psmd9 might be related to spermatogenesis.…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, high-resolution single-cell RNA-seq maps from the testes of genotypic male and pseudomale fish revealed the dynamic changes in gene expression patterns during spermatogenesis in C. semilaevis [ 15 ]. In particular, the psmd9 gene was significantly differentially expressed between pseudomales and males [ 15 ], and it was located on the Z chromosome [ 9 ], which indicates that psmd9 might be related to spermatogenesis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Role of Z Chromosome Localization Gene psmd9 in Spermatogenesis of Cynoglossus semilaevis

Zhang,

Wang,

Liu

et al. 2024

IJMS

Self Cite

View full text Add to dashboard Cite

Proteasome 26S Subunit, Non-ATPase 9 (psmd9) plays an important role in the balance of protamine and the stability of the nucleolar structure during spermatogenesis. In this study, we cloned the psmd9 of Cynoglossus semilaevis and analyzed its expression pattern. psmd9 was identified on the Z chromosome of C. semilaevis, which is considered an interesting candidate gene for spermatogenesis. qRT-PCR and FISH experiments showed that the psmd9 gene was significantly highly expressed in the testes. It is worth noting that the expression level of psmd9 in male fish testes is significantly higher than that in pseudomales. In order to further explore the role of psmd9 in spermatogenesis, a male testicular cell line was used as the experimental material. The results of the psmd9-RNAi and overexpression experiments showed that psmd9 had a synergistic effect with spermatogenesis-related genes dnd1, cfap69, dnah3 and dnajb13, but had an antagonistic effect with ccne2. Our findings offer a scientific foundation for comprehending the role of psmd9 in the spermatogenesis regulatory network of C. semilaevis.

show abstract

“…DHX37, which is situated on human chromosome 12q24.31, consists of 1157 amino acids and comprises four functional domains: namely the helicase ATP-binding domain (RecA1), the helicase superfamily C-terminal domain (RecA2), the helicase-associated domain (HA2), and the oligonucleotide/oligosaccharide-binding fold domain (OB) [21]. The RNA helicase DHX37 participates primarily in RNA-related biological processes such as transcription, splicing, ribosome biogenesis, translation, and degradation [22,23]. Numerous studies have exhibited that DHX37 is linked to developmental disorders, neuro-related diseases, and other disorders [24,25].…”

Section: Discussionmentioning

confidence: 99%

DHX37 Is a Promising Prognostic Biomarker and a Therapeutic Target for Immunotherapy and Chemotherapy in HCC

Liu,

Zhang,

Zhang

et al. 2023

Cancers

View full text Add to dashboard Cite

DHX37, a member of the DEAD/H-box RNA helicase family, has been implicated in various diseases, including tumors. However, the biological characteristics and prognostic significance of DHX37 in HCC remain unclear. In this study, we use R software 3.6.3 and multiple bioinformatics analysis tools, such as GDSC, HPA, STRING, TISCH, and TIMER2, to analyze the characterization and function of DHX37 in HCC. In addition, Western blot (WB) and immunohistochemistry (IHC) based on clinical samples validated some of the findings. DHX37 was more highly expressed in HCC samples compared to adjacent non-tumor tissues. Higher DHX37 expression is correlated with various clinicopathological characteristics in HCC, including AFP, adjacent hepatic tissue inflammation, histologic grade, T stage, and pathologic stage. Survival analysis revealed that the high DHX37 group had significantly shorter overall survival (OS), progress-free interval (PFI), and disease-specific survival (DSS) compared to the low DHX37 group. By analyzing the correlation between DHX37 and the IC50 of chemotherapeutic drugs, the results showed that DHX37 expression level was negatively correlated with the IC50 of 11 chemotherapeutic drugs. Further analysis indicated that DHX37 and its co-expressed genes may play important roles in activating the cell cycle, DNA repair, chemokine signaling pathways, and regulating the immune response, which leads to a poor prognosis in HCC. High expression of DHX37 is an independent risk factor for poor prognosis in HCC, and DHX37 is expected to be a potential target to inhibit tumor progression. Targeting DHX37 may enhance chemotherapeutic drug sensitivity and immunotherapeutic efficacy in HCC.

show abstract

Single-cell-resolution transcriptome map revealed novel genes involved in testicular germ cell progression and somatic cells specification in Chinese tongue sole with sex reversal

Cited by 12 publications

References 82 publications

Single-Cell RNA Sequencing of the Testis of Ciona intestinalis Reveals the Dynamic Transcriptional Profile of Spermatogenesis in Protochordates

Single-Cell RNA Sequencing of the Testis of Ciona intestinalis Reveals the Dynamic Transcriptional Profile of Spermatogenesis in Protochordates

The Role of Z Chromosome Localization Gene psmd9 in Spermatogenesis of Cynoglossus semilaevis

DHX37 Is a Promising Prognostic Biomarker and a Therapeutic Target for Immunotherapy and Chemotherapy in HCC

Contact Info

Product

Resources

About