Histological and Histochemical Evaluation of the Effects of Graphene Oxide on Thyroid Follicles and Gas Gland of Japanese Medaka (Oryzias latipes) Larvae

Asala, Tolulope E.; Dasmahapatra, Asok K.; Myla, Anitha

doi:10.1016/j.chemosphere.2021.131719

Cited by 10 publications

(27 citation statements)

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“…We have determined the size of the follicles as area (µm 2 ) from the digital photographs of the histological sections and analysed by imagej software. Although our data indicate a non-linear effect of GO on thyroid follicles, thyrocytes height, or GG cell distribution at the coronal end of the SB [1] , the average size of thyroid follicles in phenotypic male (testis) on 47 dph was 750.40±12.88 µm 2 whereas for females, the average size was 398.70±12.36 µm 2 . Similarly, for larvae having XY genotype, the follicular size of the thyroid was 804.60±16.32 µm 2 and those with XX genotype have 492.40 ± 16.32 µm 2 .…”

Section: Data Descriptioncontrasting

confidence: 72%

“…The animal maintenance and details of the medaka colony maintained at the Medaka fish culture facility at the Jackson State University were described previously [1] , [2] – 3 , 5 , 6] . Briefly, the breeding colony of Japanese medaka fish (orange red varieties, Hd-rR strain) was maintained in the Aquatic Toxicology Laboratory at the Plant Science building at Jackson State University, Jackson, MS, USA.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…The trunk was used for studies on gas gland cells. The phenotypic sex was determined by gonad histology and genotypic sex by genotyping sex-specific male gene dmy [1] , [2] , [3] . Description of data collection GO was obtained from Sigma (St. louis MO), and sonicated in ERM.…”

Section: Specifications Tablementioning

confidence: 99%

“…Phenotypic sex was determined histologically and the genotypic sex was identified by genotyping dmy gene. Statistical analysis was done using the GrapPad Prism software [1 , 2] . Data source location RCMI Centre for Environmental Health, Jackson State University, Jackson, MS 39217, USA Data accessibility With the article Related research article T. E. Asala, A. K. Dasmahapatra, A. Myla, P.B.…”

Section: Specifications Tablementioning

confidence: 99%

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Experimental data sets on the evaluation of graphene oxide as a thyroid endocrine disruptor and a modulator of gas gland cells in Japanese medaka (Oryzias latipes) larvae at the onset of maturity

2021

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This article presents the experimental datasets obtained from the histological/histochemical studies of endocrine disrupting effects of graphene oxide (GO) on thyroid follicles and gas gland (GG) cells of Japanese medaka larvae at the onset of maturity. The experiment was conducted on one day-post hatch (dph) starved fries (orange-red variety) immersed in different concentrations of GO (2.5-20.0 mg/L) and no GO (controls) in embryo-rearing medium (ERM) for 96 h under laboratory conditions (25 ± 1 °C; light cycle 16 h light: 8 h dark). After treatment, larvae were maintained in balanced salt solution (BSS) with food and allowed depuration for 6 more weeks in a GO-free environment. On 47 dph, the larvae were anesthetized in MS 222 and their total lengths (mm) and weights (mg) were measured, and they were then cut into three small pieces (head, trunk, and tail). Head and trunk regions were fixed in 4% PFA in 20 mM PBS for 48 h at room temperature and the post-anal tail was preserved in TRI reagent and kept at −20 °C until analysis. Tissues in 4% PFA were used for cutting 5µm thick paraffin sections in a manual rotary microtome. Sections of head regions were evaluated for thyroid follicles after hematoxylin-eosin (HE) or Periodic acid-Schiff (PAS) staining. Trunk sections were used for swim bladder (SB) inflation studies and for phenotypic sex (ovary and testis) of the larvae after HE staining. Genetic sex assessment was made from tail DNA by genotyping Y chromosome-specific male sex-determining gene dmy . Digital images were captured by using either an Olympus B-max 40 microscope attached to a camera with Q-capture Pro 7 software or an Olympus CKX53 microscope with DP22 camera and CellSens software. Images of thyroid follicles and GG cells were analyzed using imagej software. HE stained histological sections of thyroid follicles near the heart and branchial regions were captured and the area (µm 2 ) of individual follicles (minimum 3) available in the entire section were measured. The heights of thyrocytes (µm) were determined directly. Manual counting of GG cells was made from the digital images captured in several regions of the SB avoiding blood cells and other cells which have indistinct nucleus and pale cytoplasm; results were expressed as the number of GG cells/mm 2 . Data were analyzed by GraphPad prism version 7.04. For normally distributed data, one-way ANOVA followed by post-hoc Tukey's test or unpaired parametric “t” test including Welch's correction was used. Otherwise, Kruskal-Wallis test followed by nonparametric Mann-Whitney's test as a post hoc test was used. Data were expressed as means ±SEM and the level of significance was set at p < 0.05.

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Section: Data Descriptioncontrasting

confidence: 72%

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Section: Specifications Tablementioning

confidence: 99%

Section: Specifications Tablementioning

confidence: 99%

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Experimental data sets on the evaluation of graphene oxide as a thyroid endocrine disruptor and a modulator of gas gland cells in Japanese medaka (Oryzias latipes) larvae at the onset of maturity

2021

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“…16 Our laboratory has been evaluating GO, as a potential EDC using Japanese medaka fish (Oryzias latipes), as our experimental model. [17][18][19][20][21][22][23] We have demonstrated that the males of medaka fish are more sensitive to GO than the females. From our histopathological evaluation of the gonadal structures and thyroid follicles, we found that the damages observed after GO administration was mainly due to aggregation/agglomeration of GO on or within the organs rather than directly acting as an EDC.…”

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confidence: 85%

Histopathological evaluation of the interrenal gland (adrenal homolog) of Japanese medaka (Oryzias latipes) exposed to graphene oxide

Dasmahapatra

2022

Environmental Toxicology

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Due to unique physicochemical properties and wide industrial and biomedical applications, graphene oxide (GO) is ubiquitous in the aquatic ecosystem. Using Japanese medaka (Oryzias latipes) fish as a model, we previously demonstrated minimal endocrine disrupting (ED) effects of GO on reproductive organs, and thyroids. Current study investigated the ED-effects of GO on the interrenal gland (IRG) of medaka.Breeding pairs of adult male and female fish were exposed to 0 mg/L (control) or 20 mg/L GO by continuous immersion for 96 h, or to 0 or 100 μg/g GO by intraperitoneal administration. Also, 1 day post-hatch (dph) larvae were exposed to different concentrations of GO (2.5-20 mg/L) for 96 h. IRG was evaluated by immunohistochemical techniques after 21 days depuration in adults and 6 weeks in larvae. IRG cells were counted and the nuclear area was measured in hematoxylin-eosin stained sections using ImageJ software. We found that IRG is distributed adjacent to the posterior cardinal vein and its branches within the head kidney. Columnar/oval shaped periodic acid-Schiff negative, tyrosine hydroxylase positive cells are arranged either in a single, or in groups, sometimes encircling a sinusoid, or in a straight chord, laying adjacent to the endothelium of the cardinal vein, and having eosinophilic cytoplasm with round/oval basophilic nuclei. GO effect on nuclei and cell population in IRG was inconsistent; depending on exposure route, sex, and/or age of the fish. Also, because of its high adsorptive property and sharp edges, GO probably agglomerated on IRG, and induced physical injury, and ED effects.

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Per‐ and polyfluoroalkyl substances activate UPR pathway, induce steatosis and fibrosis in liver cells

Niture

Gadi

et al. 2022

Environmental Toxicology

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Per-and polyfluoroalkyl substances (PFAS), which include perfluorooctanoic acid (PFOA), heptafluorobutyric acid (HFBA), and perfluorotetradecanoic acid (PFTA), are commonly occurring organic pollutants. Exposure to PFAS affects the immune system, thyroid and kidney function, lipid metabolism, and insulin signaling and is also involved in the development of fatty liver disease and cancer. The molecular mechanisms by which PFAS cause fatty liver disease are not understood in detail. In the current study, we investigated the effect of low physiologically relevant concentrations of PFOA, HFBA, and PFTA on cell survival, steatosis, and fibrogenic signaling in liver cell models. Exposure of PFOA and HFBA (10 to 1000 nM) specifically promoted cell survival in HepaRG and HepG2 cells. PFAS increased the expression of TNFα and IL6 inflammatory markers, increased endogenous reactive oxygen species (ROS) production, and activated unfolded protein response (UPR). Furthermore, PFAS enhanced cell steatosis and fibrosis in HepaRG and HepG2 cells which were accompanied by upregulation of steatosis (SCD1, ACC, SRBP1, and FASN), and fibrosis (TIMP2, p21, TGFβ) biomarkers expression, respectively. RNA-seq data suggested that chronic exposures to PFOA modulated the expression of fatty acid/lipid metabolic genes that

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Histological and Histochemical Evaluation of the Effects of Graphene Oxide on Thyroid Follicles and Gas Gland of Japanese Medaka (Oryzias latipes) Larvae

Cited by 10 publications

References 50 publications

Experimental data sets on the evaluation of graphene oxide as a thyroid endocrine disruptor and a modulator of gas gland cells in Japanese medaka (Oryzias latipes) larvae at the onset of maturity

Experimental data sets on the evaluation of graphene oxide as a thyroid endocrine disruptor and a modulator of gas gland cells in Japanese medaka (Oryzias latipes) larvae at the onset of maturity

Histopathological evaluation of the interrenal gland (adrenal homolog) of Japanese medaka (Oryzias latipes) exposed to graphene oxide

Per‐ and polyfluoroalkyl substances activate UPR pathway, induce steatosis and fibrosis in liver cells

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