“…Using this method, the sex of 18 individuals, including young children, out of 22 examined from periods ranging from 200 to around 8000 years ago. In no case was there a discrepancy between the DNA and the morphometric studies 31 .…”
Section: Arşiv Kaynak Tarama Dergisi Archives Medical Review Journalmentioning
confidence: 92%
“…Faerman et al 31 reported from Hadassah, Jerusalem, on the establishment of a sensitive and reliable method of sex determination in skeletal remains based on amplification of singlecopy amelogenin-encoding gene (AMG). The Y allele carries a small deletion in the first intron, facilitating the design of distinct X-and Y-specific polymerase chain reactions.…”
Section: Arşiv Kaynak Tarama Dergisi Archives Medical Review Journalmentioning
Alternative approaches to sex determination of DNA samples involve investigation of regions of the amelogenin gene. This is the gene that encodes tooth enamel and is present on both the X and Y chromosomes. A review composed via Medline Internet search of literature and contributions from our experiences as well as experiences from colleagues. The rareness of failures in sex determination provides confidence in current techniques, but amelogenin gene method (singly) of sex determination is not without failures. Amelogenin PCR method/system of sex determination should not, at the moment, completely replace traditional methods of sex identification. Hence, sex identification with amelogenin gene, of subjects for forensic purposes should be conducted as much as possible through a multiple morphological-molecular combined methods to avoid fallibility of amelogenin gene.
“…Using this method, the sex of 18 individuals, including young children, out of 22 examined from periods ranging from 200 to around 8000 years ago. In no case was there a discrepancy between the DNA and the morphometric studies 31 .…”
Section: Arşiv Kaynak Tarama Dergisi Archives Medical Review Journalmentioning
confidence: 92%
“…Faerman et al 31 reported from Hadassah, Jerusalem, on the establishment of a sensitive and reliable method of sex determination in skeletal remains based on amplification of singlecopy amelogenin-encoding gene (AMG). The Y allele carries a small deletion in the first intron, facilitating the design of distinct X-and Y-specific polymerase chain reactions.…”
Section: Arşiv Kaynak Tarama Dergisi Archives Medical Review Journalmentioning
Alternative approaches to sex determination of DNA samples involve investigation of regions of the amelogenin gene. This is the gene that encodes tooth enamel and is present on both the X and Y chromosomes. A review composed via Medline Internet search of literature and contributions from our experiences as well as experiences from colleagues. The rareness of failures in sex determination provides confidence in current techniques, but amelogenin gene method (singly) of sex determination is not without failures. Amelogenin PCR method/system of sex determination should not, at the moment, completely replace traditional methods of sex identification. Hence, sex identification with amelogenin gene, of subjects for forensic purposes should be conducted as much as possible through a multiple morphological-molecular combined methods to avoid fallibility of amelogenin gene.
“…Since the DNA found in sites like these is expected to be highly degraded, only short DNA templates of up to 300bp are present [86] making it challenging for any meaningful analysis with traditional STRs. These 'minis' can prove essential where other anthropological methods cannot be applied for sex typing, phylogenies [87] and to explore the gender differences in the past populations [88,89]. Although SNPs are potential candidates for use with degraded samples, problems like low polymorphism and difficulty in mixture interpretation hinders their widespread use, outside the research setting [90].…”
Section: X-chromosomal Strs and Ministrs: Identity Testing And Beyondmentioning
The experience of using autosomal short-length amplicon STRs or miniSTRs in profiling of degraded DNA and mass disaster victims is extended into the realm of X-chromosomal (ChrX) STR miniaturization. About half of the total X-STRs are now short-length amplicons and the focus is shifting to using the mini versions of all of them. Joint multiplexing of these loci can be used for solving complex paternity cases and association of mass disaster victims with their families. This technology may herald a new dimension for research in population genetics and evolution. We present an overview of the progress made thus far and the future scope and prospects for X-miniSTRs.
“…Faerman et al (1995) [25], determined sex in skeletal remains of 18 individuals, including young children, out of 22 examined from periods ranging from 200 to around 8000 years ago based on amplification of single-copy amelogenin-encoding gene (AMG). Stone et al, (1996) [26], amplified a small fragment (112 bp) in exon 6 of the amelogenin gene to accurately determine genetic sex of 19 out of 20 ancient human skeletons dating to A. D. 1300.…”
Section: Pcr Inhibition Of Aldehydes On Dna Extracted From Human Skelmentioning
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