Sex identification in the Collared Scops Owl (Otus bakkamoena) with novel markers generated by random amplified polymorphic DNA

Wang, P. H.; Hsu, Hao-Chun; Chao, Ming-Chieh; Chan, Fang-Tse; Wang, L. M.; Lin, Po-Tsun; Tsao, Hsien-Shao; Yuan, Hsiao‐Wei; Chen, C. C.; Ding, Shih‐Torng

doi:10.1007/s12686-012-9778-3

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…The sex of these owls was initially determined by laparoscopy, and the data were then compared with the results obtained by the DNA sexing method. The results of the study by Wang et al (2013) showed that molecular methods have a great potential in determining the sex of owls, although their accuracy remains to be tested on larger cohorts. Wang et al claim that DNA testing is a safe alternative to reduce the misdiagnosis of conventional sex by laparoscopy of birds.…”

Section: Resultsmentioning

confidence: 99%

Comparative Evaluation Of Two Techniques Of Sex Determination In Lovebirds (Agapornis Spp.)

TURCU

Bel

Collarile³

et al. 2020

BUASVMCN-VM

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Most lovebirds (Agapornis spp.) have no distinct sexual dimorphic traits. The objective of the paper was to compare the results of two sexing methods, surgical sexing by celioscopy and DNA sexing by PCR from blood samples, in order to evaluate their effectiveness. Materials used to carry out the studies were a 2.7 mm telescope and endoscopy unit, surgical instruments and DNA sampling kits provided by Exomed.cz laboratory. Forty-two lovebirds were included in this study. The endoscopic sexing procedure was performed by the method described by Divers. Blood sampling for DNA testing by PCR method was performed from the metatarsal vein. The results were different in the case of one lovebird, endoscopic sexing revealed it as male, compared to DNA testing, where it was identified as female. DNA sexing is a non-invasive method that might be more accurate than celioscopy in this species, and bird owners have easier access to it.

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Section: Resultsmentioning

confidence: 99%

Comparative Evaluation Of Two Techniques Of Sex Determination In Lovebirds (Agapornis Spp.)

TURCU

Bel

Collarile³

et al. 2020

BUASVMCN-VM

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“…Various laboratory sexing analyses have been performed in studies on owl species, such as snowy owl ( Bubo scandiacus ) 32 , Indian scops owl ( Otus bakkamoena ) 33 , Tengmalm’s owl ( Aegolius funereus ) 34 , Southeast Asian barn owl ( Tyto alba javanica ) 3 , 35 , and Eurasian eagle owl ( Bubo bubo ) 36 . However, before the first molecular sexing of a given species, a primary calibration of the laboratory methods should always be done, and an appropriate primer set should be selected that gives the correct results for the studied species 37 , 38 .…”

Section: Introductionmentioning

confidence: 99%

Comparison of three primer pairs for molecular sex determination in Eurasian pygmy owls (Glaucidium passerinum)

Stehlíková Sovadinová,

Mekadim,

Korpimäki

et al. 2024

Sci Rep

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Bird sex determination is fundamental in various ecological and biological studies, although many avian species cannot be sexed visually due to their monomorphic and/or monochromatic appearance. Thus, reliable laboratory methods for sexing are a prerequisite. Most avian nestlings lack sex-related signs, including the Eurasian pygmy owl (Glaucidium passerinum). We performed laboratory sex determination analysis of this species using blood samples of 242 juveniles and nine adults. It relied on the qPCR of the specific intron from the chromo-helicase DNA-binding protein 1 gene. We tested three primer sets, the P2/P8, 2550F/2718R, and CHD1F/CHD1R, commonly used for bird laboratory sexing. The outcomes were displayed on an agarose gel electrophoresis and a plot from melt curve analysis, which had not been previously conducted in Eurasian pygmy owls. We found that only primer set CHD1F/CHD1R proved reliable, as the only one determined sex with one and two band/s and peak/s on the electrophoresis and the melt curve plot for males and females, respectively. The other two primer pairs failed and depicted one band/peak in all specimens regardless of their sex. Therefore, we recommend performing Eurasian pygmy owls’ laboratory sexing by qPCR with CHD1F/CHD1R primers only.

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Analysis of CHD Gene Polymorphism as a Model Object for Molecular Sexing of Eurasian Eagle-Owl (Bubo bubo)

Kulibaba

Liashenko

2021

Cytol. Genet.

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Sex identification in the Collared Scops Owl (Otus bakkamoena) with novel markers generated by random amplified polymorphic DNA

Cited by 3 publications

References 14 publications

Comparative Evaluation Of Two Techniques Of Sex Determination In Lovebirds (Agapornis Spp.)

Comparative Evaluation Of Two Techniques Of Sex Determination In Lovebirds (Agapornis Spp.)

Comparison of three primer pairs for molecular sex determination in Eurasian pygmy owls (Glaucidium passerinum)

Analysis of CHD Gene Polymorphism as a Model Object for Molecular Sexing of Eurasian Eagle-Owl (Bubo bubo)

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