Serum stearic acid/palmitic acid ratio as a potential predictor of diabetes remission after Roux‐en‐Y gastric bypass in obesity

Zhao, Linjing; Ni, Yan; Yu, Haoyong; Pin, Zhang; Zhao, Aihua; Bao, Yuqian; Liu, Jiajian; Chen, Tianlu; Xie, Guoxiang; Panee, Jun; Chen, Wenlian; Rajani, Cynthia; Wei, Runmin; Su, Mingming; Jia, Weiping; Wang, Jia

doi:10.1096/fj.201600927r

Cited by 29 publications

(28 citation statements)

References 60 publications

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“…Liquid chromatography coupled with mass spectrometry [ 7 , 11 , 13 , 25 , 30 – 32 , 41 – 44 , 48 , 50 , 51 ] and hydrogen nuclear magnetic resonance [ 11 , 26 , 33 , 35 , 37 – 40 , 45 – 47 , 49 ] were the most commonly used analytical platforms ( Figure 1 , Table 1 ). Gas chromatography was also a rather commonly used technique [ 7 , 27 , 28 , 34 – 37 , 42 , 44 , 52 ].…”

Section: Resultsmentioning

confidence: 99%

“…Serum (47%) [ 7 , 25 , 28 , 31 , 32 , 38 , 41 – 45 , 48 , 49 , 51 , 52 ] and plasma (25%) [ 11 , 27 , 30 , 33 , 34 , 37 ] were the most commonly used biological materials. Other analyzed samples were urine (12%) [ 13 , 26 , 37 , 39 ] and different types of tissues (16%)—heart [ 11 ], liver [ 40 , 45 ], or adipose tissue [ 28 , 50 ].…”

Section: Resultsmentioning

confidence: 99%

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Application of Metabolomics to Study Effects of Bariatric Surgery

Samczuk

Ciborowski

Krętowski

2018

Journal of Diabetes Research

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Bariatric surgery was born in the 1950s at the University of Minnesota. From this time, it continues to evolve and, by the same token, gives new or better possibilities to treat not only obesity but also associated comorbidities. Metabolomics is also a relatively young science discipline, and similarly, it shows great potential for the comprehensive study of the dynamic alterations of the metabolome. It has been widely used in medicine, biology studies, biomarker discovery, and prognostic evaluations. Currently, several dozen metabolomics studies were performed to study the effects of bariatric surgery. LC-MS and NMR are the most frequently used techniques to study main effects of RYGB or SG. Research has yield many interesting results involving not only clinical parameters but also molecular modulations. Detected changes pertain to amino acid, lipids, carbohydrates, or gut microbiota alterations. It proves that including bariatric surgery to metabolic surgery is warranted. However, many molecular modulations after those procedures remain unexplained. Therefore, application of metabolomics to study this field seems to be a proper solution. New findings can suggest new directions of surgery technics modifications, contribute to broadening knowledge about obesity and diseases related to it, and perhaps develop nonsurgical methods of treatment in the future.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Application of Metabolomics to Study Effects of Bariatric Surgery

Samczuk

Ciborowski

Krętowski

2018

Journal of Diabetes Research

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“…Evidence accumulated to date has mainly focused on palmitic acid, with little attention being paid to stearic acid-induced worsening of β-cell function. In our previous studies and other research, it has been demonstrated that, whether in the postprandial serum of T2DM patients or in the fasting serum of highfat-diet-fed mice [1][2][3][4], only stearic acid levels were profoundly increased. Meanwhile, a long-term high level of stearic acid exhibited a stronger destructive effect on β cells than that of palmitic acid [2,5].…”

Section: Introductionmentioning

confidence: 77%

“…The prevalence of T2DM has been increasing worldwide, with an estimated 336 million people currently affected [21]. It is well established that exposing pancreatic β cells to an elevated level of stearic acid causes their dysfunction, which is far advanced by the time diabetes is diagnosed clinically [1][2][3]5]. Therefore, the optimal management and prevention of T2DM should aim to ameliorate β-cell dysfunction at an early stage.…”

Section: Discussionmentioning

confidence: 99%

TCONS_00230836 silencing restores stearic acid-induced β-cell dysfunction through alleviating endoplasmic reticulum stress via a PERK/eIF2α-dependent pathway rather than apoptosis

Guo¹,

Zhang²,

Yu³

et al. 2020

Preprint

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Background: Chronic exposure of pancreatic β cells to high levels of stearic acid (C18:0) leads to impaired insulin secretion, which accelerates the progression of type 2 diabetes mellitus (T2DM). Recently, long noncoding RNAs (lncRNAs) were found to participate in saturated fatty acid-induced metabolism dysfunction. However, their contribution to stearic acid-induced β-cell dysfunction remains largely unknown. This study evaluated the possible role of the lncRNA TCONS_00230836 in stearic acid-stimulated lipotoxicity to β cells. Method: Using high-throughput RNA-sequencing, TCONS_00230836 was screened out as being exclusively differentially expressed in stearic acid-treated mouse β-TC6 cells. Co-expression network was constructed to reveal the potential mRNAs targeted for lncRNA TCONS_00230836. Changes in this lncRNA’s and candidate mRNAs’levels were further assessed by real-time PCR in stearic acid-treated β-TC6 cells and islets of mice fed a high-stearic-acid diet (HSD). The localization of TCONS_00230836 was detected by fluorescent in situ hybridization. The endogenous lncRNA TCONS_00230836 in β-TC6 cells was abrogated by its Smart Silencer. Results: The lncRNA TCONS_00230836 was enriched in mouse islets and mainly localized in the cytoplasm. Its expression was significantly increased in stearic acid-treated β-TC6 cells and HSD-fed mouse islets. Knockdown of TCONS_00230836 apparently restored stearic acid-impaired GSIS through alleviating endoplasmic reticulum stress via a PERK/eIF2α-dependent pathway. However, stearic acid-induced β-cell apoptosis was not obviously recovered. Conclusion: Our findings suggest the involvement of the lncRNA TCONS_00230836 in stearic acid-induced β-cell dysfunction, which provides novel insight into stearic acid-induced lipotoxicity to β cells. Anti-lncRNA TCONS_00230836 might be a new therapeutic strategy for alleviating stearic acid-induced β-cell dysfunction in the progression of T2DM.

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“…It is irresponsible to judge the quality of a metabolite without dose. Citrulline is an important nutritional supplement to help balance nitrogen and metabolism in the body, whereas a previous study showed that increased plasma citrulline in obesity mice may predict the development of the metabolic syndrome . Although the visceral fat volume, plasma insulin and lipid concentrations of the xylitol‐fed rats were lower than those of the high‐fat diet rats , excessive xylitol could also lead to obesity and elevated blood lipids .…”

Section: Discussionmentioning

confidence: 98%

Wulingsan (Oryeongsan/Goreisan) ameliorate lipid metabolism of obesity rats via regulation of the plasma metabolic profiling

Xue

Xue²,

Cui

et al. 2019

Biotech and App Biochem

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Objectives: Wulingsan has been used to cure disease about disorders related to fluid balance for thousands of years. The clinical practice of modern Chinese medicine has found that Wulingsan has the effect on reducing weight and fat, but its mechanism is not clear. This study investigated its effects on obesity rats and explored the underlying mechanisms by analyzing the plasma metabolic profiling. Methods: The effects of Wulingsan on obesity were evaluated with obesity rats induced by high-fat diet. Ultra high performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UPLC-Q-TOF/MS) was performed to discover potential biomarkers and evaluate whether Wulingsan could regulate these biomarkers. The levels of triglyceride (TG), total cholesterol (TC), high-density lipoprotein (HDL-C), and low-density lipoprotein (LDL-C) in serum were assessed by ELISA kits. Results: Remarkably, TG, TC, HDL-C and LDL-C in obesity rats were ameliorated after oral administration of Wulingsan. Further investigation indicated that the plasma metabolic profiles were clearly improved. Twelve potential biomarkers were identified. After intervention, these biomarkers turned back to normal level at some extent. Conclusion: The results showed that Wulingsan extract groups were normalized. Additionally, this study also showed that the metabonomics method was a promising tool to unravel how traditional Chinese medicines worked and these data can provide scientific basis for clinical application of Wulingsan.

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Serum stearic acid/palmitic acid ratio as a potential predictor of diabetes remission after Roux‐en‐Y gastric bypass in obesity

Cited by 29 publications

References 60 publications

Application of Metabolomics to Study Effects of Bariatric Surgery

Application of Metabolomics to Study Effects of Bariatric Surgery

TCONS_00230836 silencing restores stearic acid-induced β-cell dysfunction through alleviating endoplasmic reticulum stress via a PERK/eIF2α-dependent pathway rather than apoptosis

Wulingsan (Oryeongsan/Goreisan) ameliorate lipid metabolism of obesity rats via regulation of the plasma metabolic profiling

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