Serum Protein Profile of Juvenile Pacu (Piaractus mesopotamicus) and Dourado (Salminus brasiliensis) Fed Bovine Colostrum as Partial Source of Protein in the Diet

Abstract

The electrophoretic profile of serum proteins was investigated in juvenile Piaractus mesopotamicus and Salminus brasiliensis fed with diets containing bovine colostrum, a nutraceutical food, in lyophilized form (LBC) for either 30 or 60 days. Materials and Methods: Blood samples were collected from juveniles of P. mesopotamicus and S. brasiliensis fed for either 30 or 60 days with diets containing 0%, 10% or 20% of LBC. Serum protein fractions were then determined by electrophoresis in agarose gel. Results: The P. mesopotamicus, an omnivorous fish, showed six serum protein fractions, while the S. brasiliensis, a carnivorous fish, showed four serum protein fractions. In both species the albumin fraction showed higher protein content at 30 days than at 60 days (p<0.05), indicating an inverse relationship with growth. The 5 th and 4 th fraction in mobility from the P. mesopotamicus and S. brasiliensis serum, respectively, were positioned in a gamma-globulin zone. In P. mesopotamicus, the concentration of protein in the gamma-globulin zone was higher at 60 days than at 30 days (p<0.05). In S. brasiliensis, in turn, the concentration of protein in the gamma-globulin zone was higher at 30 days than at 60 days (p<0.05). In juvenile P. mesopotamicus, a higher concentration of protein was also observed in the gamma-globulin zone in the 0% LBC compared to 10% and 20% LBC (p<0.05). Thus, the feeding period influenced only the fractions that were positioned in the albumin and gamma-globulin migration zone in both species. Conclusion: The consumption of bovine colostrum decreased the concentration of protein in gamma-globulin fraction of the P. mesopotamicus, indicating that these juveniles had either less stimulus for their own synthesis of defense elements or an immunosuppressive effect of bovine colostrum ingestion.

Protein electrophoresis of non‐traditional species: A review

“…133 Agarose gel electrophoresis methods were used to examine the serum profile of the pacu and dourado, and six and four fractions were identified, respectively. 134 Changes were observed in many fractions with a modified diet over time. In the Atlantic goliath grouper, AGE methods resolved six fractions that differed by fish size, age, and sex.…”

Hematological changes in Florida pompano (Trachinotus carolinus) supplemented with β‐glucan and Pediococcus acidilactici synbiotic

“…Recent advancements have been made in EPH techniques and interpretation for many "non-traditional" species, including fishes (Cray, 2021). Here, Florida pompano exhibit six EPH fractions, similar to mammals and several other teleosts (Malinowski et al, 2020;Moretti et al, 2018;Muhammad et al, 2018). In model mammalian plasma protein electrophoretograms, fraction 1 is composed of prealbumin (mainly transthyretin, TTR), fraction 2 is composed of albumin, fractions 3 and 4 are composed of alpha-1 and alpha-2 globulins, respectively, fraction 5 is composed of beta globulins, and fraction 6 is composed of gamma globulins.…”