Serum protein pattern during cow pregnancy: Acute‐phase proteins increase in the peripartum period

Cairoli, F.; Battocchio, M.; Veronesi, M.C.; Brambilla, Daniela; Conserva, Francesca; Eberini, Ivano; Wait, Robin; Gianazza, Elisabetta

doi:10.1002/elps.200500742

Cited by 47 publications

(47 citation statements)

References 73 publications

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“…This correlation was reported by Elshourbagy et al (1985) in rats. The lowering expression of apo A-IV in cows until parturition noted in our study was also observed by Cairoli et al (2006). During lactation the concentration of apo A-IV in investigated plasma increased.…”

Section: Discussionsupporting

confidence: 86%

Changes in lipid metabolism during last month of pregnancy and first two months of lactation in primiparous cows – analysis of apolipoprotein expression pattern and changes in concentration of total cholesterol, HDL, LDL, triglycerides

Kurpińska¹,

Jarosz²,

Ożgo³

et al. 2015

Polish Journal of Veterinary Sciences

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The final weeks of pregnancy and period of increasing lactation abound with adaptive changes in the intensity of metabolic processes. Maintaining the homeostasis of an organism in prepartum and postpartum periods is the key condition in maintaining the health of the mother and the fetus/calf. The aim of the study was to analyze physiological changes in lipid metabolism in cows during the last month of first pregnancy and in the first two months of lactation, based on the expression of identified apolipoproteins and changes in selected parameters of the lipid metabolism in peripheral blood plasma. Statistically significant changes in the expression of identified apolipoproteins were observed for apolipoprotein A-1 precursor, apolipoprotein A-IV precursor, apolipoprotein E precursor and apolipoprotein J precursor. The lowest expression of the apolipoproteins was noted around parturition and higher expression was observed during the final weeks of pregnancy and during lactation. Tendencies of changes in the concentration of total cholesterol, HDL and LDL were similar in blood plasma from analyzed cows -in the last month of pregnancy a decrease was observed and subsequently an increase in the first two months of lactation was noted. In contrast to abrupt changes observed for total cholesterol, HDL and LDL, changes in concentration of triglycerides were not that extensive and during lactation this parameter was rather stable. Evaluation of changes in the analyzed parameters may contribute to a better understanding of the changes in lipid metabolism occurring in the body of pregnant and lactating young cows.

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Section: Discussionsupporting

confidence: 86%

Changes in lipid metabolism during last month of pregnancy and first two months of lactation in primiparous cows – analysis of apolipoprotein expression pattern and changes in concentration of total cholesterol, HDL, LDL, triglycerides

Kurpińska¹,

Jarosz²,

Ożgo³

et al. 2015

Polish Journal of Veterinary Sciences

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“…Thus, the transition period is the most critical time of the lactation cycle and can determine to a large measure how profitable the entire lactation will be. For this reason, many researchers have attempted to reveal complex physiological adaptive mechanisms using transcriptomic and proteomic methodologies (Madsen et al, 2002;Loor et al, 2005;Cairoli et al, 2006), the latter of which is a powerful tool for comparative analysis of complex protein mixtures based on high-resolution twodimensional electrophoresis (2-DE) coupled with mass spectrometry. This proteomic approach can provide useful information on temporal and dynamic processes occurring in the proteome.…”

mentioning

confidence: 99%

Comparative proteomics analysis of plasma proteins during the transition period in dairy cows with or without subclinical mastitis after calving

Yang¹,

Wang²,

Bu³

et al. 2012

CZECH J ANIM SCI

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ABSTRACT:The transition period is the most critical time of the cow's lactation cycle that is associated with the onset of mastitis. In this study, changes of plasma proteins in cows (n = 12) with or without subclinical mastitis after calving were determined by two-dimensional electrophoresis (2-DE), which detected 18 spots with variations in protein spots abundance. These spots were identified by liquid chromatography coupled with tandem mass spectrometry. The changes in protein profile from day 21 before calving to day 1 after calving were similar in cows with or without subclinical mastitis. Abundance of α1 acid glycoprotein (AGP) and haptoglobin was dramatically increased at parturition, while transthyretin was down-regulated at parturition, and apolipoprotein E and immunoglobulin gamma 1 were up-regulated at postpartum compared with prepartum in periparturient dairy cows. In cows infected with subclinical mastitis, AGP, haptoglobin, and serum amyloid A were dramatically increased and continued to be elevated in plasma from day 1 to day 21 after calving compared with cows free of mastitis. Changes of protein in plasma at parturition may serve as an immune system response to parturition and lactation process at the protein level and suggest that these altered proteins would not serve as a potential marker for predicting if the periparturient dairy cows are susceptible to subclinical mastitis.

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“…Its level was elevated in the blood of cows after parturition (Piccinini et al, 2004;Cairoli et al, 2006;Bionaz et al, 2007), as well as in cows suffering from mastitis (Moyes et al, 2009), respiratory disease and stress (Aich et al, 2007;Cooke and Bohnert, 2011). It may serve as a non-specific host immune response to inflammation or stress.…”

Section: Discussionmentioning

confidence: 99%

Effects of duodenal infusion of free α-linolenic acid on the plasma and milk proteome of lactating dairy cows

Yang

Wang

Yuan

et al. 2013

Animal

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This study is an exploratory analysis for understanding the effect of a duodenal infusion of an a-linolenic acid (LNA) on the plasma and milk proteome of lactating dairy cows. Four primiparous Holstein cows were fitted with duodenal cannulas and received 0, 100, 200, 300 and 400 g/day of LNA in a two-treatment crossover design. Blood and milk were collected for determination of protein composition by two-dimensional gel electrophoresis. Alteration of protein spots was detected and identified using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry (MALDI-TOF-TOF MS). Plasma haptoglobin levels, and milk b-casein A2, a s1 -casein variant and albumin, did not differ in cows after infusion of 0, 100, 200 and 300 g/day of LNA, but were increased after the cows received duodenal infusion of 400 g/day of LNA. Western blot analysis of haptoglobin expression in plasma confirmed the alterations in protein expression seen using MS. This study demonstrated that infusion of high doses of LNA by duodenal cannula can result in metabolic stress within the bovine intestine and in changes in milk composition.

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Serum protein pattern during cow pregnancy: Acute‐phase proteins increase in the peripartum period

Cited by 47 publications

References 73 publications

Changes in lipid metabolism during last month of pregnancy and first two months of lactation in primiparous cows – analysis of apolipoprotein expression pattern and changes in concentration of total cholesterol, HDL, LDL, triglycerides

Changes in lipid metabolism during last month of pregnancy and first two months of lactation in primiparous cows – analysis of apolipoprotein expression pattern and changes in concentration of total cholesterol, HDL, LDL, triglycerides

Comparative proteomics analysis of plasma proteins during the transition period in dairy cows with or without subclinical mastitis after calving

Effects of duodenal infusion of free α-linolenic acid on the plasma and milk proteome of lactating dairy cows

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