Serum prolidase activity and oxidative status in patients with bronchial asthma

Abstract

Asthma is a disease where there is an accumulation of collagen in the reticular basal membrane of the airway leading to chronic inflammation. The enzyme prolidase plays an important role in the breakdown of collagen and the breakdown of intracellular protein especially in the final stage when peptides and dipeptides contain a high level of proline. To evaluate the relationship between prolidase activity and oxidative status in asthma patients. Comparison was made between 42 patients diagnosed with bronchial asthma and 32 healthy children of similar age and gender. Serum prolidase activity was measured spectrophotometrically. Oxidative status was determined using total antioxidant capacity (TAC) and total oxidant status (TOS) measurement. The prolidase activity of the asthma patient group was statistically significant compared with the control group (P< or =0.001). TAC and TOS levels in the asthma patient group were higher than the control group (P< or =0.001, P< or =0.002, respectively). No correlation was found between the prolidase and oxidative levels of the two groups. A positive correlation was determined between the prolidase activity and TAC in the asthma patient group (P< or =0.001, r=0.501). The prolidase enzyme activity, which plays a role in the collagen turnover, was low in the asthma patients; therefore, their collagen metabolism had undergone a change and this indicates that there may be an effect on the accumulation of collagen in the reticular basal membrane. Moreover, the high level of TOS indicates that these patients were exposed to severe oxidative stress with an increased TAC response.

Prolidase function in proline metabolism and its medical and biotechnological applications

“…In addition to collagen turnover, oxidative status and oxidative stress have also been shown to correlate with prolidase activity levels under certain conditions (Aslan et al. 2007; Cakmak et al. 2009, 2010; Selek et al.…”

Serum prolidase activity in idiopathic and ischemic cardiomyopathy patients

“…However, conflicting results also have been published. In a recent article published in this journal, Cakmak et al reported that SPA concentrations were lower in asthma patients than in controls (20). Similarly, Myara et al found higher plasma prolidase levels in only 5 of 27 cirrhosis patients; they also failed to identify any correlation between the degrees of fibrosis and prolidase activity.…”

Serum Clara cell protein and atopic phenotype in children up to 2 years of age

“…After an acute injury, the respiratory epithelium is capable of regeneration without remodeling or fibrosis, and in this process, Clara cells proliferate and differentiate into ciliary epithelial cells in bronchioles, as well as in type 2 pneumocyte in alveoli, with maintenance of airway epithelium after injury . It is known that air pollution exposures occurring during the critical periods of lung growth are especially damaging, as well as that the airway epithelium in asthmatics functions abnormally and it is sensitive to oxidative damage . Repeated damage of the airway barrier with agents associated with lowering of serum CC16 during childhood (air pollution, ozone, chlorine metabolites) may increase TH2 response and the penetration of allergens in the lung, which leads to the development of atopic inhalant sensitization …”