Serum peptidome patterns of hepatocellular carcinoma based on magnetic bead separation and mass spectrometry analysis

Abstract

BackgroundHepatocellular carcinoma (HCC) is one of the most common cancers in the world,and the identification of biomarkers for the early detection is a relevant target. The purpose of the study is to discover specific low molecular weight (LMW) serum peptidome biomarkers and establish a diagnostic pattern for HCC.MethodsWe undertook this pilot study using a combined application of magnetic beads with Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technique and ClinPro Tools v2.2 to detect 32 patients with HCC, 16 patients with chronic hepatitis (CH), 16 patients with liver cirrhosis (LC) and 16 healthy volunteers.ResultsThe results showed 49, 33 and 37 differential peptide peaks respectively appeared in HCC, LC and CH groups. A Supervised Neural Network (SNN) algorithm was used to set up the classification model. Eleven of the identified peaks at m/z 5247.62, 7637.05, 1450.87, 4054.21, 1073.37, 3883.64, 5064.37, 4644.96, 5805.51, 1866.47 and 6579.6 were used to construct the peptides patterns. According to the model, we could clearly distinguish between HCC patients and healthy controls as well as between LC or CH patients and healthy controls.ConclusionsThe study demonstrated that a combined application of magnetic beads with MALDI-TOF MB technique was suitable for identification of potential serum biomarkers for HCC and it is a promising way to establish a diagnostic pattern.Virtual slidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1503629821958720.

Superoxide Dismutase1 Levels in North Indian Population with Age-Related Macular Degeneration

“…Serum was subsequently separated by centrifugation for 30 minutes at 3000 rpm. The separated serum was frozen at −80°C until analysis [ 21 , 22 ].…”

Upregulation of microRNA-25 associates with prognosis in hepatocellular carcinoma

“…Modifications of these staging systems by the addition of new biomarkers, in particular those better reflecting tumor aggressiveness, are likely to improve the prognostic assessment of HCC patients and could therefore fulfill a clinical need. Researchers have found some new prognostic markers of HCC, such as squamous cellular carcinoma antigen, SOX9, and L1 cell adhesion molecule [ 6 - 9 ].…”

Biomarker Characterization by MALDI–TOF/MS

“…Three peptides that strongly correlated with glioma were identified [243]. Serum peptide and protein profiling using magnetic beads/MALDI-TOF/MS for disease diagnosis have also been reported to diagnose hepatocellular carcinoma [244], CRC [245], schizophrenia [246], IgA nephropathy [247], biliary tract cancer [248], and inflammatory bowel diseases [249]. Moreover, this approach was used to obtain saliva peptide profiles for diagnosing primary Sj€ ogren's syndrome [250] and urine peptide profiles for diagnosing endometriosis [251].…”