Biomarker Characterization by MALDI–TOF/MS

Cho, Yi‐Tzu; Su, Hung; Wu, Deng‐Chyang; Hou, Ming‐Feng; Kuo, Chao‐Hung; Shiea, Jentaie

doi:10.1016/bs.acc.2015.01.001

Cited by 37 publications

(23 citation statements)

References 306 publications

(341 reference statements)

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“…The ions are then directed into a vacuum chamber and separated based on their TOF, which is proportional to their mass. These different flight times can then be used to generate 3D algorithms, which can have several thousand data points, with particular protein ion clusters showing as peaks [32]. Koehn et al analyzed subcellular fractions from ten oral squamous cell carcinomas and corresponding control samples using 2DE and MALDI-TOF/MS.…”

Section: Qualitative Analysismentioning

confidence: 99%

Oncoproteomics: Trials and tribulations

Zhou

Wang

et al. 2015

Proteomics Clinical Apps

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Oncoproteomics is the study of cancer-associated proteins and their interactions using proteomic technologies. It is expected to play a crucial role in the detection and surveillance of cancer, as well as in the development of personalized medicine for oncology. In this review, we will outline the current status, advantages, and limitations of oncoproteomics. Further, we will discuss available strategies arising from a better understanding of oncoproteomics, including integrated omics approaches, to overcome current problems in the discovery and validation of biomarkers, identification of novel drug targets, and management of personalized medicine.

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Section: Qualitative Analysismentioning

confidence: 99%

Oncoproteomics: Trials and tribulations

Zhou

Wang

et al. 2015

Proteomics Clinical Apps

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“…Due to its many advantages, mass spectrometry has been widely used in clinical practice. [11][12][13] In this study, we used clinically com-…”

Section: Introductionmentioning

confidence: 99%

Screening and identification of serum biomarkers of osteoarticular tuberculosis based on mass spectrometry

Chen

Jia

Lei

et al. 2020

Clinical Laboratory Analysis

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“…Since MALDI-TOF MS is, in principle, able to identify any ionizable compound [61], it can detect antibiotic resistance (e.g., via the identification of specific proteins or degradation products of antibiotics), but has also been used to reveal recombinant proteins, plasmid insertions in bacteria, or other biomarkers and diagnostic peptides in bacteria [62][63][64][65][66].…”

Section: Maldi-tof Ms Applications Beyond Species Identificationmentioning

confidence: 99%

“…As for the highly sensitive species identification, discussed above, sample processing (e.g., tryptic digestion, acidic/organic extraction, nano-liquid chromatography) can greatly increase the sensitivity and specificity of a MALDI-TOF MS method and thus enable subspecies identification [66,75]. A further possibility is the functional modification of the MALDI target plates themselves, which was for example performed with antibodies for direct immunoaffinity MALDI-TOF MS of haptoglobin or by dioxide coating in order to enrich phosphopeptides [76,77].…”

Section: Sample Processing Can Broaden the Use Of Maldi Biotypingmentioning

confidence: 99%

MALDI-TOF mass spectroscopy of yeasts and filamentous fungi for research and diagnostics in the agricultural value chain

Drissner

Freimoser

2017

Chem. Biol. Technol. Agric.

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Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS; MALDI biotyping) has become a standard tool for the accurate, rapid, and economical identification of pathogens in the clinical diagnostics laboratory. The method is continuously being improved, and new applications for distinguishing strains, identifying metabolites or functional characteristics (e.g., antibiotic resistance), and detecting microbes directly in patient samples have been developed. Adopting these methods in other disciplines than clinical diagnostics, for example, in agriculture, food safety and quality testing, or ecology, will open up new opportunities for diagnostics and research. This review focuses on MALDI-TOF MS approaches for the identification of yeasts and filamentous fungi. In contrast to bacterial diagnostics, MALDI biotyping of fungi is more challenging and less established. We thus start by discussing the role of MALDI-TOF MS as a tool for species identification; in particular with respect to DNA-based identification methods. The review then highlights the value of custom-made reference spectra for MALDI biotyping and points out recent advancements of MALDI-TOF MS, mainly from the field of clinical diagnostics that may be adopted and used for fungal diagnostic challenges. The overview ends with a summary of MALDI-TOF MS studies of yeasts and filamentous fungi of agricultural relevance.

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Biomarker Characterization by MALDI–TOF/MS

Cited by 37 publications

References 306 publications

Oncoproteomics: Trials and tribulations

Oncoproteomics: Trials and tribulations

Screening and identification of serum biomarkers of osteoarticular tuberculosis based on mass spectrometry

MALDI-TOF mass spectroscopy of yeasts and filamentous fungi for research and diagnostics in the agricultural value chain

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