Serum levels of endothelial and neural cell adhesion molecules in prostate cancer

Lynch, Donald F.; Hassen, Waleed; Clements, Mary Ann; Schellhammer, Paul F.; Wright, George L.

doi:10.1002/(sici)1097-0045(19970801)32:3<214::aid-pros8>3.0.co;2-k

Cited by 33 publications

(12 citation statements)

References 9 publications

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“…With regard to current biomarkers used in the clinic, this is the expected concentration range, giving us hope that new lung cancer markers should be detectable in serum. Through our method, we successfully identified proteins that are putative or currently used as biomarkers of lung cancer, including CEA (27,28), pro-GRP (29,31), SCC antigen (47,48), tumor M2-PK (49), NCAM (35)(36)(37), chromogranin A (29), and chromogranin B (30). In addition, we identified putative markers previously reported in lung-related proteomics studies such as member C1 of aldo-keto reductase family 1 identified by Huang et al (25) as dihydrodiol dehydrogenase and matrix metallopeptidase 1 found to be overexpressed in lung cancer patients and especially in late stage (18).…”

Section: Discussionmentioning

confidence: 99%

Identification of Five Candidate Lung Cancer Biomarkers by Proteomics Analysis of Conditioned Media of Four Lung Cancer Cell Lines

Planque

Kulasingam

Smith

et al. 2009

Molecular & Cellular Proteomics

133

107

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Detection of lung cancer at an early stage is necessary for successful therapy and improved survival rates. We performed a bottom-up proteomics analysis using a twodimensional LC-MS/MS strategy on the conditioned media of four lung cancer cell lines of different histological backgrounds (non-small cell lung cancer: H23 (adenocarcinoma), H520 (squamous cell carcinoma), and H460 (large cell carcinoma); small cell lung cancer: H1688) to identify secreted or membrane-bound proteins that could be useful as novel lung cancer biomarkers. Proteomics analysis of the four conditioned media allowed identification of 1,830 different proteins (965, 871, 726, and 847 from H1688, H23, H460, and H520, respectively). All proteins were assigned a subcellular localization, and 38% were classified as extracellular or membrane-bound. We successfully identified the internal control proteins (also detected by ELISA), kallikrein-related peptidases 14 and 11, and IGFBP2. We also identified known or putative lung cancer tumor markers such as squamous cell carcinoma antigen, carcinoembryonic antigen, chromogranin A, creatine kinase BB, progastrin-releasing peptide, neural cell adhesion molecule, and tumor M2-PK. To select the most promising candidates for validation, we performed tissue specificity assays, functional classifications, literature searches for association to cancer, and a comparison of our proteome with the proteome of lung-related diseases and serum. Five novel lung cancer candidates, ADAM-17, osteoprotegerin, pentraxin 3, follistatin, and tumor necrosis factor receptor superfamily member 1A were preliminarily validated in the serum of patients with lung cancer and healthy controls. Our results demonstrate the utility of this cell culture proteomics approach to identify secreted and shed proteins that are potentially useful as serologi-

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Section: Discussionmentioning

confidence: 99%

Identification of Five Candidate Lung Cancer Biomarkers by Proteomics Analysis of Conditioned Media of Four Lung Cancer Cell Lines

Planque

Kulasingam

Smith

et al. 2009

Molecular & Cellular Proteomics

133

107

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“…IL18Bpa is a potent inhibitor of IL-18, which is a central player in inflammation and in the immune response, and which has antineoplastic properties. ICAM-1 is expressed by leukocytes, epithelial cells, endothelial cells and tumor, stimulates neovascularization [17], and is elevated in the serum of patients with cancer [18]. IL-17 is a pro-inflammatory cytokine, that plays a crucial role in the development of autoimmunity and allergic reactions, and the expression of IL17 from Th17 is stimulated by IL23, which promotes tumor incidence and growth [19].…”

Section: Discussionmentioning

confidence: 99%

Cytokine profiling of prostatic fluid from cancerous prostate glands identifies cytokines associated with extent of tumor and inflammation

Fujita¹,

Ewing²,

Sokoll

et al. 2008

The Prostate

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BACKGROUND-Cytokines are key mediators of inflammation that may play important roles in prostate cancer initiation and progression. Cytokines found in cancerous prostates may provide further insight into the mechanisms of cancer initiation and progression, and facilitate the exploration of new markers of prostatic neoplasia and inflammation. We describe the cytokine profile of prostatic fluids obtained from cancerous prostate glands and correlate it to both cancer status and inflammation grade.

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“…ICAM1, also known as CD54, is typically expressed on endothelial cells and cells of the immune system, and plays an important role in inXammation-related process and immune surveillance (Frenette and Wagner 1996). It is expressed the highest in human lung, and its expression is also detected in normal adult prostate tissue, prostate tumor tissue and prostate cancer cells, and in serum (Nishimura and Naito 2005;Rokhlin and Cohen 1995;Lynch et al 1997). ICAM4 is exclusively expressed in erythrocytes (Hermand et al 2003) and ICAM5 mainly expressed in speciWc areas of the brain (Yoshihara et al 1994), both of which are likely involved in cellular adhesion and cell signaling events however, little experimental evidence supports a role for ICAM4 or ICAM5 in human cancer.…”

Section: Discussionmentioning

confidence: 99%

ICAM gene cluster SNPs and prostate cancer risk in African Americans

et al. 2006

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Intercellular adhesion molecules (ICAMs) are known to be involved in various human cancers. An ICAM gene cluster lying within a 26 kb region on chromosome 19p13.2, and containing ICAM1, ICAM4, and ICAM5 has recently been identified as harboring a breast and prostate cancer susceptibility locus in two populations of European ancestry from Germany and Australia. The objective of this study was to confirm the ICAM association with prostate cancer in a sample of African American prostate cancer cases (N = 286) and controls (N = 391). Six single nucleotide polymorphisms (SNPs) within the three ICAM genes were genotyped. To control for potential population stratification an ancestry-adjusted association analysis was performed. We found that ICAM1 SNPs, -9A/C (rs5490) and K469E (rs5498) were associated with prostate cancer risk in men with a family history of prostate cancer (P = 0.008). Specifically, increased risk was observed for individuals who possessed the CC genotype of the -9 A/C variant (odds ratio = 2.5; 95% CI = 1.0-6.3) and at least one G allele of non-synonymous K469E variant (odds ratio = 1.8; 95% CI = 1.2-3.1). Strong linkage disequilibrium was observed across the ICAM region (P < 0.001). A common haplotype within the ICAM gene cluster, harboring the -9A/C variant was significantly associated with prostate cancer (P = 0.03), mainly due to men with family history (P = 0.01). Our results replicate previous findings of association of the ICAM gene cluster with prostate cancer and suggest that common genetic variation within ICAM1 and not ICAM5 may be an important risk factor for prostate cancer.

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Serum levels of endothelial and neural cell adhesion molecules in prostate cancer

Cited by 33 publications

References 9 publications

Identification of Five Candidate Lung Cancer Biomarkers by Proteomics Analysis of Conditioned Media of Four Lung Cancer Cell Lines

Identification of Five Candidate Lung Cancer Biomarkers by Proteomics Analysis of Conditioned Media of Four Lung Cancer Cell Lines

Cytokine profiling of prostatic fluid from cancerous prostate glands identifies cytokines associated with extent of tumor and inflammation

ICAM gene cluster SNPs and prostate cancer risk in African Americans

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