Serum Inhibits P. aeruginosa Biofilm Formation on Plastic Surfaces and Intravenous Catheters

Hammond, Adrienne A.; Dertien, Janet; Colmer-Hamood, Jane A.; Griswold, John; Hamood, Abdul N.

doi:10.1016/j.jss.2008.09.003

Cited by 59 publications

(63 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This indicates that S. aureus is unable to form a biofilm in serum. It has been previously reported that serum can interfere with the initial attachment of bacteria to an abiotic surface (19). We therefore examined biofilm formation in plates that were washed or not washed after an initial 1-h attachment period (Fig.…”

Section: Resultsmentioning

confidence: 99%

Candida albicans and Staphylococcus aureus Form Polymicrobial Biofilms: Effects on Antimicrobial Resistance

Harriott

Noverr

2009

Antimicrob Agents Chemother

479

399

View full text Add to dashboard Cite

Candida albicans readily forms biofilms on the surface on indwelling medical devices, and these biofilms serve as a source of local and systemic infections. It is estimated that 27% of nosocomial C. albicans bloodstream infections are polymicrobial, with Staphylococcus aureus as the third most common organism isolated in conjunction with C. albicans. We tested whether S. aureus and C. albicans are able to form a polymicrobial biofilm. Although S. aureus formed poor monoculture biofilms in serum, it formed a substantial polymicrobial biofilm in the presence of C. albicans. In terms of architecture, S. aureus formed microcolonies on the surface of the biofilm, with C. albicans serving as the underlying scaffolding. In addition, S. aureus matrix staining revealed a different phenotype in polymicrobial versus monomicrobial biofilms, suggesting that S. aureus may become coated in the matrix secreted by C. albicans. S. aureus resistance to vancomycin was enhanced within the polymicrobial biofilm, required viable C. albicans, and was in part mediated by C. albicans matrix. However, the growth or sensitivity to amphotericin B of C. albicans is not altered in the polymicrobial biofilm.

show abstract

Section: Resultsmentioning

confidence: 99%

Candida albicans and Staphylococcus aureus Form Polymicrobial Biofilms: Effects on Antimicrobial Resistance

Harriott

Noverr

2009

Antimicrob Agents Chemother

479

399

View full text Add to dashboard Cite

show abstract

“…The biofilm assay was performed, as previously described, by a modified microtiter plate assay (Hammond et al, 2010;Tran et al, 2012). Seven-millimeter discs of selenium-free dental sealant or organo-selenium dental sealants were incubated in 1 mL BHI media in the presence of approximately 1,000 initial colony-forming units (CFU) of S. salivarius or 7,500 CFU of S. mutans in each well of the microtiter plate.…”

Section: Dental Sealant Materialsmentioning

confidence: 99%

Organo-Selenium-containing Dental Sealant Inhibits Bacterial Biofilm

Tran¹,

Hamood²,

Mosley³

et al. 2013

J Dent Res

View full text Add to dashboard Cite

Oral bacteria, including Streptococcus mutans and Streptococcus salivarius, contribute to tooth decay and plaque formation; therefore, it is essential to develop strategies to prevent dental caries and plaque formation. We recently showed that organo-selenium compounds covalently attached to different biomaterials inhibited bacterial biofilms. Our current study investigates the efficacy of an organo-selenium dental sealant (SeLECT-Defense TM sealant) in inhibiting S. mutans and S. salivarius biofilm formation in vitro. The organo-selenium was synthesized and covalently attached to dental sealant material via standard polymer chemistry. By colony-forming unit (CFU) assay and confocal microscopy, SeLECT-Defense TM sealant was found to completely inhibit the development of S. mutans and S. salivarius biofilms. To assess the durability of the anti-biofilm effect, we soaked the SeLECT-Defense TM sealant in PBS for 2 mos at 37°C and found that the biofilm-inhibitory effect was not diminished after soaking. To determine if organoselenium inhibits bacterial growth under the sealant, we placed SeLECT-Defense sealant over a lawn of S. mutans. In contrast to a control sealant, SeLECTDefense TM sealant completely inhibited the growth of S. mutans. These results suggest that the inhibitory effect of SeLECT-Defense TM sealant against S. mutans and S. salivarius biofilms is very effective and durable.

show abstract

“…We examined the ability of SCAA covalently attached to the inner and outer surfaces of the HDCs to inhibit AH133 biofilms using the microtiter plate assay (11,25). The SCAA coating significantly reduced biofilm biomass (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…(i) Static biofilm. The static biofilm assay was done as previously described, using the microtiter plate assay with some modifications (11,25). Biofilms were quantified by crystal violet assay as previously described (11) or by determining the CFU per catheter segment.…”

Section: Methodsmentioning

confidence: 99%

An Organoselenium Compound Inhibits Staphylococcus aureus Biofilms on Hemodialysis Catheters In Vivo

Tran

Lowry

Campbell

et al. 2012

Antimicrob Agents Chemother

Self Cite

View full text Add to dashboard Cite

Colonization of central venous catheters (CVCs) by pathogenic bacteria leads to catheter-related bloodstream infections (CRBSIs). These colonizing bacteria form highly antibiotic-resistant biofilms. Staphylococcus aureus is one of the most frequently isolated pathogens in CRBSIs. Impregnating CVC surfaces with antimicrobial agents has various degrees of effectiveness in reducing the incidence of CRBSIs. We recently showed that organoselenium covalently attached to disks as an antibiofilm agent inhibited the development of S. aureus biofilms. In this study, we investigated the ability of an organoselenium coating on hemodialysis catheters (HDCs) to inhibit S. aureus biofilms in vitro and in vivo. S. aureus failed to develop biofilms on HDCs coated with selenocyanatodiacetic acid (SCAA) in either static or flowthrough continuous-culture systems. The SCAA coating also inhibited the development of S. aureus biofilms on HDCs in vivo for 3 days. The SCAA coating was stable and nontoxic to cell culture or animals. This new method for coating the internal and external surfaces of HDCs with SCAA has the potential to prevent catheter-related infections due to S. aureus.

show abstract

Serum Inhibits P. aeruginosa Biofilm Formation on Plastic Surfaces and Intravenous Catheters

Cited by 59 publications

References 33 publications

Candida albicans and Staphylococcus aureus Form Polymicrobial Biofilms: Effects on Antimicrobial Resistance

Candida albicans and Staphylococcus aureus Form Polymicrobial Biofilms: Effects on Antimicrobial Resistance

Organo-Selenium-containing Dental Sealant Inhibits Bacterial Biofilm

An Organoselenium Compound Inhibits Staphylococcus aureus Biofilms on Hemodialysis Catheters In Vivo

Contact Info

Product

Resources

About