Organo-Selenium-containing Dental Sealant Inhibits Bacterial Biofilm

Abstract

Oral bacteria, including Streptococcus mutans and Streptococcus salivarius, contribute to tooth decay and plaque formation; therefore, it is essential to develop strategies to prevent dental caries and plaque formation. We recently showed that organo-selenium compounds covalently attached to different biomaterials inhibited bacterial biofilms. Our current study investigates the efficacy of an organo-selenium dental sealant (SeLECT-Defense TM sealant) in inhibiting S. mutans and S. salivarius biofilm formation in vitro. The organo-selenium was synthesized and covalently attached to dental sealant material via standard polymer chemistry. By colony-forming unit (CFU) assay and confocal microscopy, SeLECT-Defense TM sealant was found to completely inhibit the development of S. mutans and S. salivarius biofilms. To assess the durability of the anti-biofilm effect, we soaked the SeLECT-Defense TM sealant in PBS for 2 mos at 37°C and found that the biofilm-inhibitory effect was not diminished after soaking. To determine if organoselenium inhibits bacterial growth under the sealant, we placed SeLECT-Defense sealant over a lawn of S. mutans. In contrast to a control sealant, SeLECTDefense TM sealant completely inhibited the growth of S. mutans. These results suggest that the inhibitory effect of SeLECT-Defense TM sealant against S. mutans and S. salivarius biofilms is very effective and durable.

Do Products Preventing Demineralization Around Orthodontic Brackets Affect Adhesive Bond Strength?

“…It contains selenium which in polymer form can produce superoxide radicals [22], causing oxidative stress that damages the bacterial cell wall and DNA [23,24]. In an in vitro investigation, Tran et al, [25] demonstrated the ability of DS sealant to inhibit bacterial attachment and biofilm formation by two of the main culprits in plaque development, namely Streptococcus mutans and Streptococcus salivarius. These bacteria cannot survive under selenium-containing sealant as opposed to regular dental sealants [25].…”

“…In an in vitro investigation, Tran et al, [25] demonstrated the ability of DS sealant to inhibit bacterial attachment and biofilm formation by two of the main culprits in plaque development, namely Streptococcus mutans and Streptococcus salivarius. These bacteria cannot survive under selenium-containing sealant as opposed to regular dental sealants [25]. Furthermore, selenium-containing sealants such as DS, when polymerized, do not leach selenium and are thus believed to sustain their antibacterial effects over time.…”

The evolution of bonding in orthodontics

“…19 In 2010, Select Defense (ClassOne Orthodontics, Carlsbad, Calif) was introduced as an enamel sealant that contained selenium, an antimicrobial. Tran et al 20 reported that organo-selenium compounds covalently attached to different biomaterials, thus inhibiting bacterial biofilms. The key to protection, however, is the ability of the sealant to remain on the exposed enamel for the duration of treatment without the need for periodic reapplications.…”

Selenium-Based Drug Design

“…Thus, they suggest that the application of Se-MAP-coated gauze to the debrided tissues of burn wounds may prevent the development of biofilms and facilitate wound healing. This approach might also be used for dental applications [78] and to combat infections in hospitals caused by catheters and other medical implants.…”