Serum concentration and circadian profiles of cathepsins B, H and L, and their inhibitors, stefins A and B, in asthma

Cimerman, Nina; Brguljan, Pika Meško; Krašovec, Marta; Šuškovič, Stanislav; Kos, Janko

doi:10.1016/s0009-8981(01)00530-7

Cited by 27 publications

(13 citation statements)

References 30 publications

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“…Cystatin family 1 had broad distribution range in cells and tissues [38,39]. Cystatin B from mouse was demonstrated to prevent cerebellar apoptosis, and it expressed widely in the tissues of heart, liver, kidney, spleen, testis, brain, lung and skeletal muscle [40].…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of a Cystatin gene from Chinese mitten crab Eriocheir sinensis

Gai

Wang

et al. 2010

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 99%

Identification and characterization of a Cystatin gene from Chinese mitten crab Eriocheir sinensis

Gai

Wang

et al. 2010

Fish & Shellfish Immunology

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“…Pulmonary eosinophils are known to produce a variety of proteases, including secreted matrix metalloproteases, such as MMP-9, and -12, and lysosomal cysteine proteases, including cathepsins B, L, and S (Zheng et al, 2000;Cimerman et al, 2001;Lanone et al, 2002;Fulkerson et al, 2006). Such proteases are believed to play important roles in asthma pathogenesis, either directly in the degrading of extracellular matrix and airway remodeling or in basic inflammatory cell function.…”

Section: Discussionmentioning

confidence: 99%

Noninvasive Quantitative Tomography of the Therapeutic Response to Dexamethasone in Ovalbumin-Induced Murine Asthma

Korideck¹,

Peterson²

2009

J Pharmacol Exp Ther

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Animal models of pulmonary inflammation are critical for understanding the pathophysiology of asthma and for developing new therapies. Current conventional assessments in mouse models of asthma and chronic obstructive pulmonary disease rely on invasive measures of pulmonary function and terminal characterization of cells infiltrating into the lung. The ability to noninvasively visualize and quantify the underlying biological processes in mouse pulmonary models in vivo would provide a significant advance in characterizing disease processes and the effects of therapeutics. We report the utility of near-infrared imaging agents, in combination with fluorescence molecular tomography (FMT) imaging, for the noninvasive quantitative imaging of mouse lung inflammation in an ovalbumin (OVA)-induced chronic asthma model. BALB/c mice were intraperitoneally sensitized with OVA-Alum (aluminum hydroxide) at days 0 and 14, followed by daily intranasal challenge with OVA in phosphate-buffered saline from days 21 to 24. Dexamethasone and control therapies were given intraperitoneally 4 h before each intranasal inhalation of OVA from days 21 to 24. Twentyfour hours before imaging, the mice were injected intravenously with 5 nmol of the cathepsin-activatable fluorescent agent, ProSense 680. Quantification by FMT revealed in vivo cysteine protease activity within the lung associated with the inflammatory eosinophilia, which decreased in response to dexamethasone treatment. Results were correlated with in vitro laboratory tests (bronchoalveolar lavage cell analysis and immunohistochemistry) and revealed good correlation between these measures and quantification of ProSense 680 activation. We have demonstrated the ability of FMT to noninvasively visualize and quantify inflammation in the lung and monitor therapeutic efficacy in vivo.

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“…We observed a 10-fold decrease in cathepsin H levels in patients with inflammatory breast carcinomas compared to patients with noninflammatory carcinomas, suggesting a role for this protease in the inflammation-dependent mechanisms of this aggressive breast cancer type. In contrast, elevated levels of cathepsin H have been found to be associated with inflammatory processes in asthma, respiratory dysfunction and tobacco-induced carcinogenesis (28,34,35). It is unknown how cathepsin H is involved in these inflammatory conditions.…”

Section: Table II -Relationship Between Serum Cathepsin and Cystatin mentioning

confidence: 99%

Cathepsin B, Cathepsin H, Cathepsin X and Cystatin C in Sera of Patients with Early-Stage and Inflammatory Breast Cancer

et al. 2008

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Numerous studies have linked cathepsins and their inhibitor cystatin C to tumor invasion and metastasis. We examined whether cathepsin B, cathepsin H, cathepsin X and cystatin C could be detected in sera from women with earlystage or inflammatory breast cancer and whether they correlated with clinicopathological characteristics. Preoperative serum was obtained from 176 patients with early-stage breast cancer (tumor size ≤5 cm, negative lymph nodes) and 31 patients with inflammatory breast cancer. Cathepsin and cystatin C levels were measured by ELISA. The patient and tumor characteristics under study were age at diagnosis, menopausal status, tumor size, tumor grade, and steroid hormone receptor status. Serum cathepsin B levels were significantly lower in patients with poorly differentiated tumors. High cystatin C levels were associated with tumor size, postmenopausal status and patient age. Interestingly, significantly lower levels of cathepsin X and H were found in patients with inflammatory breast cancer, a trend also observed for cathepsin B and cystatin C.

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Serum concentration and circadian profiles of cathepsins B, H and L, and their inhibitors, stefins A and B, in asthma

Cited by 27 publications

References 30 publications

Identification and characterization of a Cystatin gene from Chinese mitten crab Eriocheir sinensis

Identification and characterization of a Cystatin gene from Chinese mitten crab Eriocheir sinensis

Noninvasive Quantitative Tomography of the Therapeutic Response to Dexamethasone in Ovalbumin-Induced Murine Asthma

Cathepsin B, Cathepsin H, Cathepsin X and Cystatin C in Sera of Patients with Early-Stage and Inflammatory Breast Cancer

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