Identification and characterization of a Cystatin gene from Chinese mitten crab Eriocheir sinensis

Olive flounder (Paralichthys olivaceus) cystatin B: Cloning, tissue distribution, expression and inhibitory profile of piscine cystatin B

“…S2). Therefore, our results show that PoCystatin B may be involved in innate immunity, consistent with the data in previous studies in leech (Theromyzon tessulatum, Lefebvre et al, 2004), Chinese mitten crab (Eriocheir sinensis, Li et al, 2010), turbot (S. maximus, Xiao et al, 2010, and rock bream (O. fasciatus, Premachandra et al, 2012).…”

“…Among marine organisms, the expression of turbot S. maximus cystatin B (SmCytB) was highest in muscle, brain, heart, and liver, and lowest in spleen, blood, gill, and kidney (Xiao et al, 2010). The mRNA transcripts of the Chinese mitten crab Eriocheir sinensis type 1 cystatin (EsCystatin) could be detected in all tested tissues, including hemolymph, gill, hepatopancreas, gonad, muscle, and heart, and the highest expression level was observed in muscle, 20-fold that of the levels in heart (Li et al, 2010).…”

Comparative transcriptome reveal the potential adaptive evolutionary genes in Andrias davidianus

“…Similar results were found in topmouth culter Erythroculter ilishaeformis and zebrafish Danio rerio , in which 38 candidate genes exhibited signs of positive selection with dN/dS ratios > 0.5 [ 6 ]. Five genes related to the immune system [ 26 – 29 ] [cystatin-like, oncostatin-M-specific receptor subunit beta isoform X1(OSMF), exonuclease, cell death regulator Aven, and centromere protein H] were detected in the A. davidianus / H. chinensis and A. davidianus / N. viridescens groups. Andrias davidianus is aquatic and inhabiting subterranean rivers and caves while N. viridescens and H. chinensis are mainly terrestrial and only special stage in water.…”

The Anti-Angiogenic Activity of a Cystatin F Homologue from the Buccal Glands of Lampetra morii

“…At present, the cystatins are usually expressed in the Pichia pastoris ( P. pastoris ) or insect cells probably due to the relatively complex structures, which might affect their expression and folding [13,16]. Actually, cystatins were usually expressed as inclusion bodies in the E. coli [33,34]. Although some studies used pEGX-4T-1, pSmart-I, or pET32a vector to obtain the soluble cystatins in the E. coli , the recombinant cystatins usually contain a tag with a relatively larger molecular weight that needs to be removed or set in an empty plasmid control to eliminate its effects on the functions of the recombinant cystatins [35,36,37].…”

“…In the present study, rLm-cystatin F was found to inhibit the activity of papain, which is a classic cysteine protease (EC 3.4.22.2), suggesting that rLm-cystatin F possessed the basic functions of cystatins [45]. At the similar conditions, rLm-cystatin F showed a better inhibitory effect on the activity of papain when compared with rEsCystatin, which was a recombinant protein identified from the Chinese mitten crab ( Eriocheir sinensis ) [34]. In 2011, Xie and colleagues showed that sv-cystatin from Naja naja atra was able to suppress the growth, invasion, and metastasis of B16F10 cells and MHCC97H cells by reducing the activity of cathepsin B, matrix metalloproteinase-2 (MMP-2), and matrix metalloproteinase-9 (MMP-9), and inhibition of epithelial-mesenchymal transition [16,17].…”