Serum A1 and B apolipoprotein determination: comparison of an immunoturbidimetric method with a monoclonal-antibody-based radial immunodiffusion assay

Abstract

Epidemiological and clinical evidence have indicated that apolipoprotein A1 and B determination can better define the lipoprotein pattern in normal subjects and in subjects with coronary heart disease. In this paper, a recent immunoturbidimetric method for routine apolipoprotein A1 and B measurement (using the Turbitimer system and commercially available antisera) has been evaluated. The precision and the accuracy of the method have been previously considered. Within-run and between-run coefficients of variation (ranging from 1.67% to 5.04%) for both assays indicate good precision of the method. Accuracy was evaluated on 2 consecutive days (n = 10 each run) using a standard serum for apolipoprotein A1 and B. The bias obtained was 3.79% for apolipoprotein A1 and 2.30% for B. Apolipoproteins A1 and B were then measured in 100 normal and hyperlipemic sera with the immunoturbidimetric assay and radial immunodiffusion (using the monoclonal antibodies). The data obtained were evaluated by linear regression analysis (Al, r = 0.893; B, r = 0.862). The good correlation between the two methods suggests that the immunoturbidimetric assay can be usefully performed for routine apolipoprotein A1 and B determination because of its lower cost, rapidity, and simplicity.

The application of ultra‐performance liquid chromatography/tandem mass spectrometry to the detection and quantitation of apolipoproteins in human serum

“…These lipoprotein particles are associated with a variety of proteins, including the apolipoprotein (Apo) families with the most abundant proteins in HDL and LDL being Apo A1 and Apo B100, respectively 2, 3. The concentration of these two proteins in serum can be identified very rapidly using immunoturbidometric assays on clinical analysers 4. However, this process requires specific antibodies to individual proteins to gain quantitative information.…”

Lp(a), ApoB and ApoB/ApoA1 Ratio as Potential Serum Markers for Predicting Early-Onset Acute Coronary Syndrome

“…ApoA1 and ApoB levels were measured using turbidimetry assay technique (immunoturbidimetry) as per manufacturers protocol (ERBA Mannheim). 11 Their serum levels were subsequently applied to derive ApoB/ApoA1 ratio. Lp(a) was quantitated similarly by immunoturbidimetry.…”

Advanced Lipoprotein Testing and Subfractionation Are Clinically Useful

“…85 All rely on the ability of an antibody to recognize a specific amino acid sequence (epitope) on the apoprotein. This method not only requires a consistently high degree of quality in the antibody production but also recognition that other factors may affect the ability of the antibody to recognize the epitope.…”