Serotonergic Ligands Induce Spawning but not Oocyte Maturation in the Bivalve <i>Mactra chinensis</i> from Central Japan

Fong, Peter P.; Deguchi, Ryusaku; Kyozuka, Keiichiro

doi:10.2307/1543058

Cited by 33 publications

(15 citation statements)

References 20 publications

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“…Conversely, in bivalves, the 5-HT triggers the egg-laying. In these molluscs, occurrence of monoaminergic fibres have been observed (Osada et al, 1992;Fong et al, 1994Fong et al, , 1996 as well as serotonergic receptors in gonads and on the membranes of muscular cells (Matsutani and Nomura, 1986;Khotimchenko, 1989). Comparative observations between Sepia officinalis and bivalves show that (1) the origin of 5-HT in the genital tract is different from the nervous system for bivalves (Khotimchenko and Deridovich, 1991); from the follicular cells and full-grown oocytes for Sepia officinalis, (2) as well as the targets: oocytes for bivalves (Guerrier et al, 1996) and oviduct for S. officinalis.…”

Section: Discussionmentioning

confidence: 99%

Evidence of 5-hydroxytryptamine synthesis in the follicles ofSepia officinalis and direct involvement in the control of egg-laying

et al. 2000

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Section: Discussionmentioning

confidence: 99%

Evidence of 5-hydroxytryptamine synthesis in the follicles ofSepia officinalis and direct involvement in the control of egg-laying

et al. 2000

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“…Kyozuka et al, 1997), limpets (Gould et al, 2001) and nemertean worms (Stricker and Smythe, 2000). Stimulation with 5-HT caused repetitive Ca 2+ transients in Mactra oocytes, although the drug had a weak effect on triggering GVBD (see also Fong et al, 1996). The 5-HTinduced Ca 2+ oscillations proceeded without external Ca 2+ in contrast to the situation observed at fertilization.…”

Section: Discussionmentioning

confidence: 96%

External Ca2+ is predominantly used for cytoplasmic and nuclear Ca2+ increases in fertilized oocytes of the marine bivalveMactra chinensis

Deguchi

Morisawa

2003

Journal of Cell Science

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IntroductionFully-grown oocytes arrested at the first meiotic prophase (prophase I, PI) in ovaries progress oocyte maturation, when exposed to hormones or released from inhibitory substances, to acquire the ability for fertilization in most animal species. These oocytes are again arrested at species-specific stages including the first metaphase (metaphase I, MI), second metaphase (metaphase II, MII), and pronuclear stage (PN), until fertilization (Masui, 1985). In the bivalves such as Mytilus and Ruditapes, the second arrest of meiosis occurs at MI prior to fertilization (MI-type) (Masui, 1985;Osanai and Kuraishi, 1988). In contrast, there are some bivalve species (e.g. Spisula and Mactra) in which meiosis reinitiation from PI is physiologically triggered concomitantly with fertilization without a process of oocyte maturation (PI-type) (Masui, 1985;Deguchi and Osanai, 1994b). Regardless of the stages of fertilization, single or multiple increases in intracellular Ca 2+ in fertilized oocytes or eggs have been detected in all animal species investigated so far (reviewed by Jaffe, 1985;Miyazaki et al., 1993;Stricker, 1999). The Ca 2+ increases are recognized as essential for the oocytes or eggs to be released from the cell cycle arrest (for a review, see Whitaker and Patel, 1990).As for MI-type bivalves, temporal patterns of Ca 2+ increases at fertilization have been analyzed in five different species: Mytilus, Crassostrea, Ruditapes, Limaria and Hiatella. When the Ca 2+ indicator Fluo-3 is introduced as AM ester, only a single blunt Ca 2+ increase, which persists for several minutes, is observed in fertilized oocytes of Mytilus (Abdelmajid et al., 1993) and Ruditapes (Leclerc et al., 2000). In the oocytes injected with Ca 2+ indicators such as Fura-2 and Calcium Green-1, however, Ca 2+ response at fertilization comprises an initial sharp Ca 2+ transient and subsequent repetitive Ca 2+ spikes (Ca 2+ oscillations) in all of the five species, including Mytilus and Ruditapes (Deguchi and Osanai, 1994a;Deguchi and Morisawa, 1997). In Mytilus, the initial Ca 2+ transient at fertilization arises almost synchronously in the oocyte without forming a point-source Ca 2+ wave (Deguchi and Osanai, 1994a). A more recent analysis revealed that the increased Ca 2+ 367Oocytes of the marine bivalve Mactra chinensis are spawned and arrested at the germinal vesicle stage (first meiotic prophase) until fertilization, without undergoing a process called oocyte maturation. As is the case of other animals, a fertilized oocyte of the bivalve displays increases in intracellular free Ca 2+ . We have clarified here the spatiotemporal patterns and sources of the intracellular Ca 2+ changes at fertilization. Shortly after insemination, increased Ca 2+ simultaneously appeared at the whole cortical region of the oocyte and spread inwardly to the center, attaining the maximal Ca 2+ levels throughout the oocyte, including the cytoplasm and nucleus. The initial maximal Ca 2+ peak was followed by a submaximal plateau phase of cytoplasmic and nuclear...

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“…In Japanese clam [122], 1, 10, 20, 50, 100, and 1000 μM α-methyl-5-HT injected into the foot induces spawning in 0, 25, 31, 63, 75, and 100% of specimens, respectively, compared to 0% in control and 100% in ≥20 μM 5-HT. In addition, Japanese clams injected with 10, 100, and .…”

Section: Receptor-mediated 5-ht Stimulation Of Spawning In Bivalvesmentioning

confidence: 93%

Pharmacology and Molecular Identity of Serotonin Receptor in Bivalve Mollusks

Alavi¹,

Nagasawa²,

Takahashi³

et al. 2017

Serotonin - A Chemical Messenger Between All Types of Living Cells

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It is now known that 5-HT regulates several neurobehavioral systems such as mood, appetite, sleep, learning, and memory. It also plays critical roles in the physiological functions of peripheral organs involved in stress, growth, and reproduction in the animal kingdom. 5-HT content has seen to be higher in the nervous system of bivalves than those of other examined invertebrates and vertebrates. Thus, bivalves have been considered as an excellent model to investigate 5-HT functions in neurological and peripheral systems. The present study reviews knowledge on 5-HT signaling mediated through 5-HT receptor and its physiological contribution to regulate reproduction in bivalves. Two G-protein-coupled 5-HT 1-like receptors have been cloned in bivalve species. However, binding affinities of the 5-HT agonists and antagonists to the isolated plasma membrane proteins and their effects on spawning in bivalves suggest the presence of a single or mixed 5-HT 1-, 5-HT 2-, and 5-HT 3-like receptors. It has suggested that the 5-HT-like receptors in bivalves are distinct from those of mammalian 5-HT receptors due to pharmacological properties. The present review pays a special attention to future research perspectives to better understand 5-HT regulation of reproduction in bivalves, which can provide us with satisfactory knowledge to elucidate reproductive disorders associated with dysfunctions of the neurotransmitter system.

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Serotonergic Ligands Induce Spawning but not Oocyte Maturation in the Bivalve Mactra chinensis from Central Japan

Cited by 33 publications

References 20 publications

Evidence of 5-hydroxytryptamine synthesis in the follicles ofSepia officinalis and direct involvement in the control of egg-laying

Evidence of 5-hydroxytryptamine synthesis in the follicles ofSepia officinalis and direct involvement in the control of egg-laying

External Ca2+ is predominantly used for cytoplasmic and nuclear Ca2+ increases in fertilized oocytes of the marine bivalveMactra chinensis

Pharmacology and Molecular Identity of Serotonin Receptor in Bivalve Mollusks

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