Evidence of 5-hydroxytryptamine synthesis in the follicles ofSepia officinalis and direct involvement in the control of egg-laying

Zatylny, Céline; Durantou, F.; Boucaud‐Camou, E.; Henry, Joël

doi:10.1002/(sici)1098-2795(200002)55:2<182::aid-mrd7>3.0.co;2-b

Cited by 21 publications

(10 citation statements)

References 22 publications

(23 reference statements)

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“…To date, there are only few methods to quantitatively measure biogenic monoamines (5‐HT, DA, NE) and their metabolites in the brains of cephalopods. Previously published methods, fluorometry (Juorio, ; Juorio and Killick, ) and HPLC‐ECD (Kime and Messenger, ; Zatylny et al , ; Jozet‐Alves et al , ), might be tedious and time consuming but , overall, are either limited to the detection of only one or two biogenic monoamines or do not quantify their metabolites. Considering (a) the importance of cephalopods in the field of Neurosciences (Hochner and Shomrat, ), (b) the fact that 5‐HT, NE and DA are closely physiologically linked, and (c) the necessity to quantify both the monoamine and its metabolite(s) to assess its turnover and, consequently, its brain modulation, a reliable and accurate method designed to quantify at the same time monoamines and their metabolites in the cephalopod brain seems to be of upmost importance.…”

Section: Introductionmentioning

confidence: 99%

An HPLC‐ECD method for monoamines and metabolites quantification in cuttlefish (cephalopod) brain tissue

Bidel

Corvaisier

Jozet‐Alves

et al. 2016

Biomedical Chromatography

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The cuttlefish belongs to the mollusk class Cephalopoda, considered as the most advanced marine invertebrates and thus widely used as models to study the biology of complex behaviors and cognition, as well as their related neurochemical mechanisms. Surprisingly, methods to quantify the biogenic monoamines and their metabolites in cuttlefish brain remain sparse and measure a limited number of analytes. This work aims to validate an HPLC-ECD method for the simultaneous quantification of dopamine, serotonin, norepinephrine and their main metabolites in cuttlefish brain. In comparison and in order to develop a method suitable to answer both ecological and biomedical questions, the validation was also carried out on a phylogenetically remote species: mouse (mammals). The method was shown to be accurate, precise, selective, repeatable and sensitive over a wide range of concentrations for 5-hydroxyindole-3-acetic acid, serotonin, dopamine, 3,4-dihydroxyphenylacetic acid and norepinephrine in the both extracts of cuttlefish and mouse brain, though with low precision and recovery for 4-hydroxy-3-methoxyphenylethylene glycol. Homovanillic acid, accurately studied in rodents, was not detectable in the brain of cuttlefish. Overall, we described here the first fully validated HPLC method for the routine measurement of both monoamines and metabolites in cuttlefish brain. Copyright © 2016 John Wiley & Sons, Ltd.

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Section: Introductionmentioning

confidence: 99%

An HPLC‐ECD method for monoamines and metabolites quantification in cuttlefish (cephalopod) brain tissue

Bidel

Corvaisier

Jozet‐Alves

et al. 2016

Biomedical Chromatography

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“…Serotonin released from neurons in ganglia may bind directly to receptors on muscle cells to induce contractions. Likewise, it has been shown that ovarian muscle contraction is an important process in the movement of gametes prior to egg-laying or spawning (Howard and Talbot 1992;Zatylny and Boucaud-Camou 1999;Zatylny et al 2000). For example, in the lobster, 5-HT can induce signiWcant ovarian muscle contraction (Howard and Talbot 1992).…”

Section: A Putative Truncated 5-ht Receptor In Abalonementioning

confidence: 99%

“…In molluscs, bioassays, as well as electrophysiological, immunocytochemical and genetic studies, have implicated it in behaviors and physiological processes such as sensation, feeding, development, and locomotion (Rathouz and Kirk 1988;Croll 1988; Barlow and Truman 1991;Kuhl et al 1992;Satterlie et al 1995). Serotonin has consistently been detected in the neural tissue of molluscs including abalone (Panasophonkul et al 2004), and it also plays an important role in reproduction through the biosynthesis and release of active egg-laying peptides precursor proteins, gamete maturation, and the control and initiation of gamete release in several other molluscan species (Ram et al 1992;Krantic et al 1993;Fong et al 1994;Ouimet and Castellucci 1997;Vaca and Alfaro 2000;Zatylny et al 2000). Administration of 5-HT has been shown to induce spawning in bivalves (Ram et al 1992;Matsutani and Nomura 1982;Gibbons and Castagna 1984;Hirai et al 1988).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone

Panasophonkul

Apisawetakan

Cummins

et al. 2009

Histochem Cell Biol

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In molluscs, the neurotransmitter serotonin (5-HT) has been linked to a variety of biological roles including gamete maturation and spawning. The possible involvement of 5-HT in abalone gamete release was demonstrated by a dose-dependent increase in Haliotis rubra gonad contractile bioactivity following 5-HT stimulation. Physiological functions associated with 5-HT, are mediated through binding to 5-HT receptors. A cDNA encoding a putative 5-HT receptor consisting of 359 amino acids was isolated from the tropical abalone H. asinina, termed 5-HT(1 ha). The 5-HT(1 ha) shares G-protein-coupled receptor motifs with metazoan 5-HT receptors, including predicted transmembrane domains, active sites for protein kinase action, and N-linked glycosylation sites. However, the third intracellular loop of 5-HT(1 ha) is relatively short, and only six transmembrane domains are predicted, implying a truncated receptor. Phylogenetic analysis with known 5-HT receptor genes suggests that 5-HT(1 ha) belongs to the type 1 5-HT receptor family. Expression analysis by RT-PCR showed that 5-HT(1 ha) mRNA was present in all tissues examined, including the neural ganglia and gonad tissues. Immunocytochemistry revealed the presence of 5-HT(1 ha) specifically within the soma of neuronal cells located in the outer cortex of both cerebral and pleuropedal ganglia. In ovarian and testicular tissues, 5-HT(1 ha) immunoreactivity was observed in epithelial cells of the outer capsule and connective tissue of the trabeculae to which the gamete follicles adhere. Whether this receptor transcript is translated to a functional protein needs to be verified, but if so, it could play a role in reproduction.

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“…They are contractile and produce a gelatinous coat which covers the egg as a second egg capsule as it is released into the mantle cavity by the oviduct (Zatylny et al, 2000). Rajasekharan et al (2005) reported that, the gland nidamental of Sepia pharaonis has certain structural features of a secretory organ containing a number of tubules filled with dense population of coccoid and rod shaped bacteria.…”

Section: Study Of the Morphology And Parasitic Infection Of S Pharaomentioning

confidence: 99%

Study of the Morphology and Parasitic Infection of the Cuttlefish, Sepia Pharaonis Ehrenberg, 1831 (Cephalopoda: Sepiidae) in the Marine Water Off Kuwait

Al-Behbehani

2006

Egypt. J. of Aquatic Biolo. and Fish.

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Evidence of 5-hydroxytryptamine synthesis in the follicles ofSepia officinalis and direct involvement in the control of egg-laying

Cited by 21 publications

References 22 publications

An HPLC‐ECD method for monoamines and metabolites quantification in cuttlefish (cephalopod) brain tissue

An HPLC‐ECD method for monoamines and metabolites quantification in cuttlefish (cephalopod) brain tissue

Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone

Study of the Morphology and Parasitic Infection of the Cuttlefish, Sepia Pharaonis Ehrenberg, 1831 (Cephalopoda: Sepiidae) in the Marine Water Off Kuwait

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