Serosurvey of tick-borne pathogens in dogs from urban and rural areas from Parana State, Brazil

Vieira, Thállitha Samih Wischral Jayme; Vieira, Roberval Daiton; Nascimento, Denise Amaral Gomes do; Tamekuni, Kátia; Toledo, Roberta dos Santos; Chandrashekar, Ramaswamy; Marcondes, Mary; Biondo, Alexander Welker; Vidotto, Odilon

doi:10.1590/s1984-29612013000100019

Cited by 42 publications

(47 citation statements)

References 26 publications

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“…The true prevalence of seropositive dogs for B. vogeli was 40.0% (64/160; IC 95 40.0% -59.2%), lower than those previously reported for São Paulo (42.4%), Belo Horizonte (66.9%) and Paraná State (46.4%) (4,22,23) . Lower seroprevalence data (35.7%) were obtained from a population of dogs from a veterinary hospital in the same study area (7) .…”

Section: Resultsmentioning

confidence: 55%

“…Although ticks were not identified in this study, the vector is very likely to be R. sanguineus, which is considered the main species observed in dogs bred in urban areas in Brazil (4,5,44) . This suspicion is supported because, in this survey in Lavras (MG), there was a predominance of urban indoor dogs (138/160; 86%).…”

Section: Resultsmentioning

confidence: 80%

“…Hematological changes during infection are highlighted by thrombocytopenia and leukopenia (2,3) . Considered a cosmopolitan disease, CME is particularly prevalent in tropical and subtropical regions, with seroprevalence between 0.7 and 86.2% (4) . In Brazil, the occurrence of canine ehrlichiosis has been increasing significantly in different regions and coincides with the distribution of R. sanguineus, which is widely distributed in many urban areas in the country (5) .…”

Section: Introductionmentioning

confidence: 99%

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HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

et al. 2017

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The aim of this study was to determine the prevalence of anti-Ehrlichia canis and anti-Babesia vogeli IgG antibodies in dogs and correlate this prevalence with risk factors to evaluate the relation of serological status to hematological findings. Blood samples of dogs attended from September 2011 to March 2012 at the veterinary hospital of the Federal University of Lavras, Brazil, were analyzed using an indirect fluorescence antibody test (IFAT). Of the total 160 dog serum samples, 23.7% (38 dogs; CI 95 17.7% -30.7%) were seropositive for E. canis, 40.0% (64 dogs; CI 95 40.0% -59.2%) for B. vogeli, and 5.6% (9 dogs) for both hemoparasites. None of the epidemiological variables showed a significant association (P>0.05) with seropositivity to E. canis and B. vogeli. Dogs seropositive for E. canis showed lower values for hematocrit (P<0.05). However, for the erythrogram, the platelet count, the leukogram and clinical signs, no significant difference (P>0.05) was observed between dogs that were seropositive and seronegative for E. canis or for B. vogeli. Serological results suggest that infection with E. canis and B. vogeli is endemic in the canine population in question, with a prevalence of the subclinical phase (asymptomatic) in dogs that are seropositive for ehrlichiosis or babesiosis. Keywords: babesiosis; canine; ehrlichiosis; IFAT; laboratory findings. ResumoO objetivo deste estudo foi determinar a prevalência de anticorpos IgG anti-Ehrlichia canis e antiBabesia vogeli em cães e correlacionar com a prevalência e fatores de risco para avaliar a relação do estado sorológico com os achados hematológicos. Amostras de sangue de cães coletadas de setembro de 2011 a março de 2012, no Hospital Veterinário da Universidade Federal de Lavras, Brasil, foram analisadas usando o teste de reação de imunofluorescência indireta (RIFI). Do total de amostras de soro de 160 cães, 23,7% (38 cães; IC95 17,7% -30,7%) foram soropositivos para E. canis, 40,0% (64 cães; IC95 40,0% -59,2%) para B. vogeli, e 5,6% (9 cães) para os dois hemoparasitas. Nenhuma das variáveis epidemiológicas mostrou associação significativa (P> 0,05) com a soropositividade para E. canis e B. vogeli. Cães soropositivos para E. canis mostraram valores médios mais baixos para hematócrito (P<0,05). No entanto, para o eritrograma, a contagem de plaquetas, o leucograma e os sinais clínicos, nenhuma diferença significativa (P> 0,05) foi observada entre cães soropositivos e soronegativos para E. canis ou para B. vogeli. Os resultados sorológicos deste estudo sugerem que a Cienc. anim. bras., Goiânia, v.18, 1-9, e-36095, 2017 2 FONSECA, J.P., et al.infecção por E. canis e B. vogeli é endêmica na população canina em questão, com uma prevalência da fase subclínica (assintomática) em cães soropositivos para erliquiose ou babesiose.

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Section: Resultsmentioning

confidence: 55%

Section: Resultsmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

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HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

et al. 2017

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“…found in dogs from Campo Grande showed 99% of identity with B. vogeli, which has already been found in dogs in the Brazilian states of Minas Gerais (PASSOS et al, 2005;COSTA-JÚNIOR et al, 2009), Pernambuco (RAMOS et al, 2010, Goiás (DUARTE et al, 2011) and Mato Grosso (SPOLIDORIO et al, 2011). Serological surveys revealed that the prevalence of antibodies to B. vogeli in dogs in Brazil ranged from 28.7% (COSTA-JÚNIOR et al, 2009) to 46.4% (VIEIRA et al, 2013). Also, B. gibsoni has been detected in dogs in the state of Paraná (TRAPP et al, 2006).…”

Section: Igg Antibodies Tomentioning

confidence: 60%

Molecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantumin Mato Grosso do Sul, Brazil

Sousa

André

Herrera³

et al. 2013

Rev. Bras. Parasitol. Vet.

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Tick-borne pathogens affect a wide range of vertebrate hosts. To identify tick-borne pathogens among dogs from Campo Grande, MS, Brazil testing seropositive for Leishmania infantum (syn. L. chagasi), a serological and molecular study was conducted to detect Ehrlichia canis, Anaplasma platys and Babesia vogeli in 60 serum and spleen samples. A confirmatory diagnosis of L. infantum based on serological and molecular assays was also performed, as was sequence alignment and phylogenetic analysis to assess the identity of the parasite species infecting these animals. IgG antibodies to Ehrlichia spp., B. vogeli and L. infantum were found, respectively, in 39 (65%), 49 (81.6%) and 60 (100%) of the sampled dogs. Twenty-seven (45%), fifty-four (90%), fifty-three (88.3%), two (3.3%) and one (1.6%) dog were positive, respectively, for E. canis, Leishmania spp., Leishmania donovani complex, Babesia sp. and Anaplasma sp. in PCR assays. After sequencing, the amplicons showed 99% of identity with E. canis, B. vogeli, A. platys and Leishmania chagasi isolates. The findings of this study indicate that L. infantum-seropositive dogs from Campo Grande are exposed to multiple tick-borne pathogens, which should therefore be included in the differential diagnosis of dogs with clinical suspicion of leishmaniasis.Keywords: Ehrlichia canis, Babesia vogeli, Anaplasma platys, Leishmania infantum, dogs, co-infection. ResumoPatógenos transmitidos por carrapatos atingem uma variedade de hospedeiros vertebrados. Para identificar os agentes patogênicos transmitidos por carrapatos entre cães soropositivos para Leishmania infantum no município Campo Grande-MS, foi realizado um estudo sorológico e molecular para a detecção de Ehrlichia canis, Anaplasma platys e Babesia vogeli em 60 amostras de soro e baço, respectivamente. Adicionalmente, foi realizado o diagnóstico confirmatório de L. infantum por meio de técnicas sorológicas e moleculares. Também foi realizado o alinhamento e análise filogenética das sequências para indicar a identidade das espécies de parasitas que infectam esses animais. Anticorpos IgG anti-Ehrlichia spp., anti-B. vogeli e anti-L. infantum foram detectados em 39 (65%), 49 (81,6%) e 60 (100%) dos cães amostrados, respectivamente. Vinte e sete (45%), cinquenta e quatro (90%), cinquenta e três (88,3%), dois (3,3%) e um (1,6%) cães mostraram-se positivos na PCR para E. canis, Leishmania spp., Leishmania donovani complex, Babesia sp. e Anaplasma sp., respectivamente. Após o seqüenciamento, os amplicons mostraram 99% de similaridade com isolados de E. canis, B. vogeli e A. platys e Leishmania chagasi. Os resultados deste estudo indicaram que os cães soropositivos para L. infantum de Campo Grande, MS, são expostos a vários agentes transmitidos por carrapatos, e, portanto, devem ser incluídos no diagnóstico diferencial em cães com suspeita clínica de leishmaniose.

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“…(LABRUNA et al, 2000;SZABÓ et al, 2001;COSTA et al, 2013). This scenario has resulted in B. vogeli and E. canis infection prevalence to be generally higher in urban than in rural dogs SPOLIDORIO et al, 2013;VIEIRA et al, 2013) because the ecologic niche of R. sanguineus s.l. in Brazil is typically in human dwellings (LABRUNA & PEREIRA, 2001).…”

Section: Introductionmentioning

confidence: 99%

Survey of canine tick-borne diseases in Lábrea, Brazilian Amazon: ‘accidental’ findings of Dirofilaria immitis infection

Soares

Camargo

Gennari

et al. 2014

Rev. Bras. Parasitol. Vet.

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Blood samples were collected from 99 domestic dogs from the urban and rural areas of the Lábrea municipality, state of Amazonas, Brazil. Canine serum samples were tested by immunofluorescence assay against Rickettsia spp., which revealed that only 3.0% (1/33) and 7.6% (5/66) of the dogs from urban and rural areas, respectively, reacted positively to at least one Rickettsia species. DNA was extracted from canine blood and tested by a battery of PCR assays targeting protozoa of the genera Babesia and Hepatozoon, and bacteria of the genera Rickettsia and Ehrlichia and family Anaplasmataceae. All samples were negative in the PCR assays targeting the genera Babesia, Hepatozoon, Ehrlichia and Rickettsia. For Anaplasmataceae, 3% (1/33) and 39.4% (26/66) of the urban and rural dogs, respectively, yielded amplicons that generated DNA sequences 100% identical to the corresponding sequence of Wolbachia endosymbiont of Dirofilaria immitis. Because of these results, all canine DNA samples were further tested in a PCR assay targeting filarial nematodes, which was positive for 18.2% (6/33) and 57.6% (38/66) urban and rural dogs, respectively. Filarial-PCR products generated DNA sequences 100% identical to D. immitis. While tick-borne infections were rare in Lábrea, D. immitis infection rates were among the highest reported in South America.Keywords: Dogs, ticks, Rickettsia, Wolbachia, Dirofilaria immitis, Amazon. ResumoAmostras de sangue foram coletadas de 99 cães domésticos de áreas urbana e rural do município de Lábrea, estado do Amazonas. Soros caninos foram testados pela técnica de imunofluorescência indireta contra Rickettsia spp., resultando em apenas 3,0% (1/33) e 7,6% (5/66) de cães soropositivos nas áreas urbana e rural, respectivamente. DNA foi extraído do sangue canino e testado por diferentes protocolos da PCR para detecção de protozoários dos gêneros Babesia e Hepatozoon, e bactérias dos gêneros Rickettsia e Ehrlichia e da família Anaplasmataceae. Todas as amostras foram negativas nos protocolos de PCR para os gêneros Babesia, Hepatozoon, Ehrlichia e Rickettsia. Para Anaplasmataceae, 3% (1/33) e 39,4% (26/66) dos cães de áreas urbana e rural, respectivamente, geraram sequências de DNA 100% idênticas ao endosimbionte Wolbachia de Dirofilaria immitis. Posteriormente, as amostras foram testadas pela PCR para nematódeos filarídeos, resultando em 18,2% (6/33) e 57,6% (38/66) de amostras positivas nas áreas urbana e rural, respectivamente. Os produtos geraram sequências de DNA 100% idênticas a D. immitis. Em contraste com várias outras regiões do Brasil, infecções transmitidas por carrapatos foram raras em Lábrea. Por outro lado, as frequências de infecção por D. immitis estiveram entre as mais altas relatadas na América do Sul.

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Serosurvey of tick-borne pathogens in dogs from urban and rural areas from Parana State, Brazil

Cited by 42 publications

References 26 publications

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

HEMATOLOGICAL PARAMETERS AND SEROPREVALENCE OF Ehrlichia canis AND Babesia vogeli IN DOGS

Molecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantumin Mato Grosso do Sul, Brazil

Survey of canine tick-borne diseases in Lábrea, Brazilian Amazon: ‘accidental’ findings of Dirofilaria immitis infection

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