Seroprevalences of Rickettsia conorii, Ehrlichia canis and Coxiella burnetii in Dogs from Montenegro

Laušević, Dejan; Ilić, Tamara; Nenadović, Katarina; Bacić, Dragan; Obrenović, Sonja

doi:10.2478/s11686-019-00098-w

Cited by 15 publications

(14 citation statements)

References 43 publications

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“…Interestingly, in 2012, a higher antibody positivity (8.0%) was found in dogs with owner comparing with those from the municipal kennel (4.6%) (data not shown). These findings are discordant with those obtained in Montenegro where a higher positivity was obtained in shelter dogs [37]. Additionally, in the United States, California, a higher positivity was observed in stray dogs than in dogs with owner [28].…”

Section: Discussionsupporting

confidence: 68%

Coxiella burnetii in Dogs and Cats from Portugal: Serological and Molecular Analysis

et al. 2022

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Dogs and cats are potential sources of infection for some zoonotic diseases such as Q fever, caused by Coxiella burnetii, a multiple host pathogen. Q fever outbreaks in dogs and cats have been related with parturition and abortion events, and ticks have a potential role in the transmission of this pathogen. This study aimed to screen for C. burnetii in dogs and cats, and in ticks collected from infested animals. An observational descriptive study was conducted in Portugal at two time points nine years apart, 2012 and 2021. Sera obtained from dogs and cats (total n = 294) were tested for C. burnetii antibodies using a commercial ELISA adapted for multi-species detection. C. burnetii DNA was screened by qPCR assay targeting IS1111 in uterine samples and in ticks. A decrease in the exposure to C. burnetii was observed in cats from 17.2% (95% CI: 5.8–35.8%) in 2012 to 0.0% in 2021, and in dogs from 12.6% (95% CI: 7.7–19.0%) in 2012 to 1.7% (95% CI: 0.3–9.1%) in 2021 (p < 0.05). Overall, and despite differences in the samples, rural habitat seems to favour the exposure to C. burnetii. The DNA of C. burnetii was not detected in ticks. The low seropositivity observed in 2021 and the absence of C. burnetii DNA in the tested samples, suggest that dogs and cats from Portugal are not often exposed to the pathogen. Nevertheless, the monitoring of C. burnetii infection in companion animals is an important tool to prevent human outbreaks, considering the zoonotic potential for owners and veterinarians contacting infected animals, mainly dogs and cats from rural areas which often come into contact with livestock.

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Section: Discussionsupporting

confidence: 68%

Coxiella burnetii in Dogs and Cats from Portugal: Serological and Molecular Analysis

et al. 2022

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“…This VBP has been previously identified in 38.6% of red foxes from BiH (Hodžić et al., 2015) and less commonly by molecular means in dogs sampled in Serbia and Croatia (Gabrielli et al., 2015; Vojta et al., 2009). Unexpectedly, pathogens vectored by Rhipicephalus sanguineus sensu lato, such as A. platys and H. canis , were detected in dog population herein examined but not E. canis , despite its presence in dogs from neighbouring countries such as Montenegro (Laušević et al., 2019).…”

Section: Discussionmentioning

confidence: 79%

“…Other canine VBPs that are prevalent in this country include the Oriental eyeworm Thelazia callipaeda , a zoonotic nematode parasite implicated in occasional human infections in Eastern Europe (Colella et al., 2016; Hodžić et al., 2014; Paradžik et al., 2016), and the apicomplexan pathogen Hepatozoon canis (Hodžić et al., 2015). However, there have been no reports to date of bacterial VBPs infecting Bosnian canines, likely due to a dearth of research given that evidence of such pathogens has been found in neighboring countries (Huber et al., 2017; Laušević et al., 2019). In addition, rickettsial pathogens have been molecularly detected in Bosnian ticks, including the zoonotic bacterial species Anaplasma phagocytophilum (Hodžić et al., 2017), the causative agent of potentially lethal human granulocytic anaplasmosis.…”

Section: Introductionmentioning

confidence: 99%

High‐throughput microfluidic real‐time PCR for the simultaneous detection of selected vector‐borne pathogens in dogs in Bosnia and Herzegovina

Colella

Huggins

Hodžić

et al. 2022

Transbounding Emerging Dis

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A scarcity of information on the occurrence of zoonotic vector‐borne pathogens (VBPs), alongside a lack of human and animal health authorities’ awareness of pre‐existing data, augment the risk of VBP infection for local people and limit our ability to establish control programs. This holds especially true in low‐middle income countries such as Bosnia and Herzegovina (BiH). This dearth of information on zoonotic VBPs is bolstered by the inability of previously used diagnostic tests, including conventional molecular diagnostic methods, to detect the full spectrum of relevant pathogens. Considering this, we set out to apply a microfluidic qPCR assay capable of detecting 43 bacterial and protozoan pathogens from blood to accrue critical baseline data for VBPs occurrence in BiH. A total of 408 dogs were tested of which half were infected with at least one VBP of zoonotic or veterinary importance. Leishmania infantum was found in 18% of dogs, reaching a prevalence as high as 38% in urbanized areas of Sarajevo. These data highlight substantially higher levels of L. infantum prevalence when compared to that previously reported using conventional methods using the same samples. Additionally, this high‐throughput microfluidic qPCR assay was able to detect pathogens rarely or never reported in canines in BiH, including Anaplasma phagocytophilum (3%), Anaplasma platys (0.2%), haemotropic Mycoplasma (1%) and Hepatozoon canis (26%). Our report of the endemicity of important zoonotic pathogens and those of clinical significance to dogs emphasizes the need for urgent implementation of surveillance and control for VBPs in BiH, targeting both animal and human infections within the country.

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“…Ixodidae ailesine bağlı keneler insan ve hayvan sağlığını etkileyen çok sayıda hastalık etkenine vektörlük yapma potansiyeline sahiptirler (Yücesan et al, 2019). Bu etkenler arasında zoonotik önemi olan Coxiella burnetii (Cikman et al, 2017) ve son zamanlarda zoonoz olduğu düşünülen Ehrlichia canis (Laušević et al, 2019) bakterileri de yer almaktadır.…”

Section: Discussionunclassified

“…E. canis'in daha önce sadece köpekleri enfekte ettiği düşünülüyordu ancak, Venezuela'da bir grup asemptomatik insandan da izole edilip moleküler olarak tanımlanmıştır. Böylece E. canis, insanlar için de klinik hastalıkların potansiyel bir ajanı olarak düşünülmüştür (Laušević et al, 2019). E. canis, Akdeniz'e kıyısı olan tüm Avrupa ülkelerinde endemiktir (Santoro et al, 2016).…”

Section: Introductionunclassified

Kene Isırığı Şikâyetiyle Hastaneye Başvuran Hastalardan Çıkarılan Kenelerde Coxiella burnetii ve Ehrlichia canis’in Moleküler Yöntemlerle Araştırılması

Ekici

Gürbüz²,

Halidi

et al. 2021

Commagene Journal of Biology

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Ticks have to suck blood from their hosts during their developmental stages, except for the egg period. They also transmit the pathogens to their host while feeding. A total of 100 ticks obtained from 42 female and 58 male patients admitted to a hospital were included in the study. After the morphological examination of the ticks, DNA extraction was performed. Conventional PCR for Coxiella burnetii and Nested-PCR for Ehrlichia canis were performed using species-specific primers. In the morphological examination, it was determined that all of the ticks belonged to the Ixodidae family and 95 (95%) were Rhipicephalus sanguineus and 5 (5%) were Ixodes ricinus species. C. burnetii was detected in 36 ticks and E. canis was found in 26 ticks out of 100 ticks included in the study. It was determined that 33 (91.67%) of the C. burnetii infected ticks were R. sanguineus and 3 (8.33%) were I. ricinus. It was also determined that 25 (96.15%) of the E. canis infected ticks were R. sanguineus and one (3.85%) was the I. ricinus. When the incidence of C. burnetii and E. canis was examined according to the place of residence of the patients, it was found that C. burnetii was seen at a higher rate in people living in rural areas and as a result of the statistical analysis, it was found that the difference was statistically significant. In conclusion, ticks pose a significant risk as vectors of C. burnetii and E. canis.

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Seroprevalences of Rickettsia conorii, Ehrlichia canis and Coxiella burnetii in Dogs from Montenegro

Cited by 15 publications

References 43 publications

Coxiella burnetii in Dogs and Cats from Portugal: Serological and Molecular Analysis

Coxiella burnetii in Dogs and Cats from Portugal: Serological and Molecular Analysis

High‐throughput microfluidic real‐time PCR for the simultaneous detection of selected vector‐borne pathogens in dogs in Bosnia and Herzegovina

Kene Isırığı Şikâyetiyle Hastaneye Başvuran Hastalardan Çıkarılan Kenelerde Coxiella burnetii ve Ehrlichia canis’in Moleküler Yöntemlerle Araştırılması

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