Seroprevalence of Seven Zoonotic Pathogens in Pregnant Women from the Caribbean

Wood, Heidi; Drebot, Michael A.; Dewailly, Éric; Dillon, Liz; Dimitrova, Kristina; Forde, Martin; Grolla, Allen; Lee, Elise; Loftis, Amanda D.; Makowski, Kai; Morrison, Karen E.; Robertson, Lyndon; Krecek, R.C.

doi:10.4269/ajtmh.14-0107

Cited by 36 publications

(42 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is evident that as soon as children begin to attend elementary school at age 6, the IgG seroprevalence rate increases. Similar associations between rates of DENV seropositivity age of children have been reported in studies conducted in several cities in Asian and Latin American [10, 46–49]. However, the relative vulnerability of children compared to adults is still a subject of considerable debate [50–52], and needs further investigation.…”

Section: Discussionsupporting

confidence: 54%

Dengue seroprevalence, seroconversion and risk factors in Dhaka, Bangladesh

Dhar-Chowdhury¹,

Paul²,

Haque³

et al. 2017

PLoS Negl Trop Dis

Self Cite

View full text Add to dashboard Cite

BackgroundDengue virus (DENV) activity has been reported in Dhaka, Bangladesh since the early 1960s with the greatest burden of dengue fever and dengue hemorrhagic fever cases observed in 2000. Since this time, the intensity of dengue activity has varied from year to year, and its determining factors remained relatively unknown. In light of such gaps in knowledge, the main objectives of this study were to determine the magnitude of seroprevalence and seroconversion among the surveyed population, and establish the individual/household level risk factors for the presence of DENV antibodies among all age groups of target populations in the city of Dhaka.Methodology/Principal findingsConsidering the lack of fine scale investigations on the factors driving dengue activity in Bangladesh, a prospective cohort study involving serological surveys was undertaken with participant interviews and blood donation across the city of Dhaka in 2012. Study participants were recruited from 12 of 90 wards and blood samples were collected during both the pre-monsoon (n = 1125) and post-monsoon (n = 600) seasons of 2012. The findings revealed that the seroprevalence in all pre-monsoon samples was 80.0% (900/1125) while the seropositivity in the pre-monsoon samples that had paired post-monsoon samples was 83.3% (503/600). Of the 97 paired samples that were negative at the pre-monsoon time point, 56 were positive at the post-monsoon time point. This resulted in a seroprevalence of 93.2% (559/600) among individuals tested during the post-monsoon period. Seroprevalence trended higher with age with children exhibiting a lower seropositivity as compared to adults. Results from this study also indicated that DENV strains were the only flaviviruses circulating in Dhaka in 2012. A multivariate analysis revealed that age, possession of indoor potted plants, and types of mosquito control measures were significant factors associated with DENV seroprevalence; while attendance in public/mass gatherings, and use of mosquito control measures were significantly associated with DENV seroconversion after adjusting for all other variables.Conclusions/SignificanceOur study suggests that there is a high level of endemic dengue virus circulation in the city of Dhaka which has resulted in significant DENV seroprevalence among its residents. Seropositivity increased with age, however, a substantial proportion of children are at risk for DENV infections. Our serological analysis also documents considerable DENV seroconversion among study participants which indicates that a large proportion of the population in the city of Dhaka were newly exposed to DENV during the study period (pre-and post-monsoon 2012). High levels of seroconversion suggest that there was an intense circulation of DENV in 2012 and this may have resulted in a significant risk for viral associated illness. Findings of our study further indicated that home-based interventions, such as removing indoor potted plants and increased bed net use, in addition to vector control measures in public parks...

show abstract

Section: Discussionsupporting

confidence: 54%

Dengue seroprevalence, seroconversion and risk factors in Dhaka, Bangladesh

Dhar-Chowdhury¹,

Paul²,

Haque³

et al. 2017

PLoS Negl Trop Dis

Self Cite

View full text Add to dashboard Cite

show abstract

“…The sensitivity of the rLipL32 IgG ELISA was found to be 100% when compared to the MAT assay, suggesting its potential use in assessing the seroprevalence of leptospirosis in the community (24,25). Meanwhile, the sensitivity and specificity of the rLipL32 IgM ELISA were lower than in earlier reports (8,26,27).…”

Section: Discussioncontrasting

confidence: 43%

Recombinant LipL32 Protein Developed Using a Synthetic Gene Detects Leptospiraspecific Antibodies in Human Serum Samples

Yaakob

Rodrigues

Opook

et al. 2017

MJMS

View full text Add to dashboard Cite

Background: Synthetic biology is emerging as a viable alternative for the production of recombinant antigens for diagnostic applications. It offers a safe alternative for the synthesis of antigenic principles derived from organisms that pose a high biological risk.Methods: Here, we describe an enzyme-linked immunosorbent assay (ELISA) using the synthetic recombinant LipL32 (rLipL32) protein expressed in Escherichia coli for the detection of Leptospira-specific antibodies in human serum samples. The rLipL32-based ELISA was compared with a microscopic agglutination test (MAT), which is currently used as the gold standard for the diagnosis of leptospirosis.Results: Our results showed that all the MAT-positive serum samples were positive for Leptospira-specific IgG in an ELISA, while 65% (n = 13) of these samples were also positive for Leptospira-specific IgM. In the MAT-negative serum samples, 80% and 55% of the samples were detected as negative by an ELISA for Leptospira-specific IgM and IgG, respectively.Conclusion: An ELISA using the synthetic rLipL32 antigen was able to distinguish Leptospira-specific IgM (sensitivity 65% and specificity 80%) and IgG (sensitivity 100% and specificity 55%) in human serum samples and has the potential to serve as a rapid diagnostic test for leptospirosis.

show abstract

“…Infectious diseases, particularly sexually transmitted infections (Snead et al, 2017; Lewis-Bell et al, 2013) and vector-borne diseases (Brown, Vickers, Salas, & Smikle, 2009; Wood et al, 2014), are of concern in the Jamaican population. For example, studies have reported 100% seroprevalence of dengue virus antibodies in the general population (Brown et al, 2009) and among pregnant women (Wood et al, 2014) in Jamaica.…”

Section: Introductionmentioning

confidence: 99%

“…For example, studies have reported 100% seroprevalence of dengue virus antibodies in the general population (Brown et al, 2009) and among pregnant women (Wood et al, 2014) in Jamaica. Compared to other Caribbean countries, pregnant women in Jamaica have higher seroprevalence of bacterial infections, including leptospirosis and spotted fever group rickettsioses (SGFR) (Wood et al, 2014). The relatively high prevalence of vector borne diseases coincides with poor knowledge and prevention practices in some areas in Jamaica (Alobuia, Missikpode, Aung, & Jolly, 2015).…”

Section: Introductionmentioning

confidence: 99%

Maternal Exposures Associated with Autism Spectrum Disorder in Jamaican Children

Christian

Samms‐Vaughan

Lee

et al. 2018

J Autism Dev Disord

View full text Add to dashboard Cite

Autism spectrum disorder (ASD) is a complex neurodevelopmental disorder with poorly understood etiology. Many maternal exposures during pregnancy and breastfeeding potentially interfere with neurodevelopment. Using data from two age- and sex-matched case-control studies in Jamaica (n = 298 pairs), results of conditional logistic regression analyses suggest that maternal exposures to fever or infection (matched odds ratio (MOR) = 3.12, 95% CI 1.74-5.60), physical trauma (MOR 2.02, 95% CI 1.01-4.05), and oil-based paints (MOR 1.99, 95% CI 1.14-3.46) may be associated with ASD. Additionally, maternal exposure to oil-based paints may modify the relationship between maternal exposure to pesticides and ASD, which deepens our understanding of the association between pesticides and ASD.

show abstract

Seroprevalence of Seven Zoonotic Pathogens in Pregnant Women from the Caribbean

Cited by 36 publications

References 24 publications

Dengue seroprevalence, seroconversion and risk factors in Dhaka, Bangladesh

Dengue seroprevalence, seroconversion and risk factors in Dhaka, Bangladesh

Recombinant LipL32 Protein Developed Using a Synthetic Gene Detects Leptospiraspecific Antibodies in Human Serum Samples

Maternal Exposures Associated with Autism Spectrum Disorder in Jamaican Children

Contact Info

Product

Resources

About