2017
DOI: 10.21315/mjms2017.24.5.5
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Recombinant LipL32 Protein Developed Using a Synthetic Gene Detects Leptospiraspecific Antibodies in Human Serum Samples

Abstract: Background: Synthetic biology is emerging as a viable alternative for the production of recombinant antigens for diagnostic applications. It offers a safe alternative for the synthesis of antigenic principles derived from organisms that pose a high biological risk.Methods: Here, we describe an enzyme-linked immunosorbent assay (ELISA) using the synthetic recombinant LipL32 (rLipL32) protein expressed in Escherichia coli for the detection of Leptospira-specific antibodies in human serum samples. The rLipL32-bas… Show more

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(2 citation statements)
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“…The study was approved by the Medical Research and Ethical Committee, Ministry of Health Malaysia (study number NMRR-13-677-15713). A detailed protocol for the production of synthetic recombinant LipL32 protein was previously described (Yaacob et al, 2017).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The study was approved by the Medical Research and Ethical Committee, Ministry of Health Malaysia (study number NMRR-13-677-15713). A detailed protocol for the production of synthetic recombinant LipL32 protein was previously described (Yaacob et al, 2017).…”
Section: Methodsmentioning
confidence: 99%
“…Determination of the immunodominant region in LipL32 by ELISA. Optimized amount of purified rLipL32 (0.25 μg/50 µl well), NrLipL32 (0.5 μg/50 µl well), CrLipL32 (0.5 μg/50 µl well), and the intermediate LipL32 fragment (0.125 μg/50 µl well) were used to coat 96-well MaxiSorp immunoassay plates (Nunc, Denmark), and the ELISA was performed as previously described (Yaacob et al, 2017). In order to determine the concentrations of antibodies in the serum samples, a standard curve was obtained using human IgM and IgG (25-0.0488 ng/ml).…”
Section: Determination Of the Lipl32 Antigenic Regions Using In Silicmentioning
confidence: 99%