Seroprevalence of culicoides-borne disease in cattle in European Turkey

Abstract

Summary:In this research, seroprevalence of BTV serotypes (BTV-4, BTV-9, BTV-16), and occurrence of Akabane and Ephemeral fever viruses were investigated in 557 cattle sera collected from 21 locations in European Turkey (Thrace). Virus neutralization test was used to discriminate antibody response against BTV serotypes while commercial blocking-ELISA systems were used to detect antibodies for Akabane and ephemeral fever viruses. The overall seroprevalence of BTV was recorded as 73.54%, comprising individual percentages for BTV-4, BTV-9 and BTV-16 were 69.04%, 71.38% and 80.2%, respectively. The seroprevalence of Ephemeral fever virus infection in Thrace varied between 2.5 and 15.3% on the province basis, whilst antibody percentage for Akabane virus was found as low as 0.14% in the study.Key words: Akabane, bluetongue, ephemeral fever, seroprevalence. Trakya bölgesinde sığırlarda sivrisineklerle nakledilen enfeksiyonların seroprevalansıÖzet: Trakya bölgesinde, 2003 yılında 5 ile bağlı 21 ilçe/köyden toplam 557 sığır, Mavidil, Akabane ve Ephemeral Fever enfeksiyonlarının seroprevalanslarının tespiti amacıyla random sampling metoduyla örneklendi. Serum örnekleri Mavidil virusunun 3 farklı serotipine (BTV-serotip 4, BTV-serotip 9 ve BTV-serotip 16) karşı virus nötralizasyon testine tabi tutuldu. Aynı serum örneklerine Akabane ve Ephemeral Fever virus antikorlarının tespiti için blocking ELISA testi uygulandı. Trakya bölgesinde mavi dil enfeksiyonunun seroprevalansı %73.54 olarak tespit edilirken, BTV serotip-4 %69.04, BTV serotip-9 %71.38, BTV serotip-16 ise %80.2 olarak saptandı. Akabane enfeksiyonunun seroprevalansı Trakya bölgesinde %0.14 olarak belirlenirken, Ephemeral fever enfeksiyonunun seroprevalansı örneklenen iller bazında %2.5-15.3 olarak tespit edildi.

The origin and molecular characterization of the BEF virus caused by the small-scale epidemic in Western Turkey

“…The one in 2008 was limited to the south of the country while the one in 2012 was much wider and spread towards the northwest of the country (Aziz-Boaron et al, 2012;Tonbak et al, 2013;Karayel-Hacıoglu et al, 2021). The serological studies show that, the virus appeared to be circulated in the north and European part of the country (Karaoglu et al, 2007). Reported that, no reported until now of BEFV was obtained from Aegean region located in the west of the country with necessary climate conditions for vectors while a serological study carried out in two provinces in the region found no seropositivity (Erol et al, 2015).…”

“…Following the first statement, epidemics were reported to have occurred every 2-4 years in south and southeast regions of Turkey (Aziz-Boaron et al, 2012). The occurring epidemics and serological studies show that the virus is common around middle, south, southeast, north and northwest parts of the country (Karaoglu et al, 2007;Albayrak and Ozan, 2010;Aziz-Boaron et al, 2012;Tonbak et al, 2013;Karayel-Hacıoglu et al, 2021). During all these years, no statement about BEFV has been reported in Aegean region, which is located in the west of Turkey and which has suitable conditions and climate for vector activation.…”