Serotypic identification of local Bluetongue virus isolates using Plaque Reduction Neutralization (PRN) and Reverse Transcriptase Polymerase Chain Reaction (RTPCR) Techniques
Abstract:Summary:Bluetongue virus (BTV) is a vector-borne disease of ruminants disseminated in the tropics and sub-tropics. It is also an important problem in the Middle East. The purpose of this study is serotypic identification of local Bluetongue viruses (BTV) isolated between 1998 and 2005. For this purpose, generic (g) and serotype specific (s) RT-PCR systems and the Plaque Reduction Neutralization Assay (PRNA) were used. Generic and serotype specific RT-PCR was performed on the segment 10 and segment 2 levels, re… Show more
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